Gefitinib (ZD1839)

製品コードS1025

Gefitinib (ZD1839)化学構造

分子量(MW):446.90

Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。

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  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

製品安全説明書

EGFR阻害剤の選択性比較

生物活性

製品説明 Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。
特性 A potent EGFR tyrosine kinase inhibitor.
靶点
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro試験

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzRTWM2OD1yLkC1OEDPxE1? NXvvWnlnOTZ3MU[0O|M>
NIH3T3 M4HET2Z2dmO2aX;uJGF{e2G7 NXTWXWp7OC5{IN88US=> NGPOV4gzKGh? M2T1V2ROW09? MYrJcohq[mm2aX;uJI9nKEWJRjDzeIlufWyjdHXkJIh2dWGwIHXyZmIyKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODF2NDFOwG0> MkPhNVY1QDB{OES=
COS7 NGn4TWxHfW6ldHnvckBCe3OjeR?= NInofYU6PiEQvF2= MXfJcohq[mm2aX;uJI9nKGi3bXHuJGpCUzJiVk[xO2YhdXW2YX70JIV5eHKnc4Pl[C=> NVHL[ot[OTdzN{i3NlI>
A549 NV;ZenhbS3m2b4TvfIlkKEG|c3H5 M{\KOlk3KGh? MkLBSG1UVw>? NV64ZYNkUUN3ME2xN{42QSEQvF2= Ml\ONVg{OTN6MEe=
K562 NYPPeoFDS3m2b4TvfIlkKEG|c3H5 M3v2Xlk3KGh? MlWxSG1UVw>? Ml\PTWM2OD17LkO2JO69VQ>? NXOyZ5UyOTh|MUO4NFc>
MDA-MB-231 NVGycmxPS3m2b4TvfIlkKEG|c3H5 NXrrfXJlQTZiaB?= NWHEdIJ3TE2VTx?= MX7JR|UxRTN4Lk[xJO69VQ>? NHrtUpEyQDNzM{iwOy=>
MOLT4 NYnFOIhVS3m2b4TvfIlkKEG|c3H5 MWS5OkBp MnvLSG1UVw>? MmfwTWM2OD1zNT6wNkDPxE1? NYfBPYxuOTh|MUO4NFc>
MCF7 NYS4fHJ4S3m2b4TvfIlkKEG|c3H5 NIrrbFA2OCEQvF2= NYDuR4RSPzJiaB?= MnTNTWM2OD1zMj6wOUDPxE1? MUWxPFc4OThzOR?=
BT474 NHiyOmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljLO|IhcA>? M4q0PWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS0O|Qh[2WubIOgc5ZmemW6cILld5NqdmdiRWLCZlIhf2m2aDDFR|UxKG:oIECuN{DPxE1? NXPKdXZJOTlyMki0NlQ>
HN5 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\STpA3PzJiaB?= NH:2fplCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjOOUBk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJJdqfGhiRVO1NEBw\iByLkCxJO69VQ>? NUO1UFhsOTlyMki0NlQ>
BA/F3 NVHQS2F2S3m2b4TvfIlkKEG|c3H5 NFHX[Zk4OiCq M2e0[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiRVfGVkBNQDV6UjDteZRidnRid3n0bEBKSzVyIH;mJFAvODFyODFOwG0> MXSxPVI{QTJ{OR?=
NIH3T3 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYi3NkBp MV7Hdo94fGhiaX7obYJqfGmxbjDv[kBud3W|ZTDOTWgwO1R|IHPlcIx{KGW6cILld5NqdmdiZYLCNkBo\W6nIIfpeIghUUN3MDDv[kAxNjV2IN88US=> MWSxPVY3PTN5Nx?=
MCF7 MX NXrFb3k4TnWwY4Tpc44hSXO|YYm= Mnj1N|AhdWmw Mlj0TY5pcWKrdHnvckBw\iCEQ2LQJIV5eHKnc4Pl[EB4cXSqIFnDOVAhd2ZiMT6xJO69VQ>? M3fLSVE6QTN{OU[w
A431 M1LSeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPz[WkyOCEQvF2= NVfzZmNPPzJiaB?= NWnlVHp4T0l3ME2wMlUyPCEQvF2= NYHWXHhROTl7Nkm0OlU>
A431 Ml;KT4lv[XOnIFHzd4F6 NGPsUZMxNjFizszN NWfJ[IJtOSCq MlPaTY5pcWKrdHnvckBw\iCHR1[tbY5lfWOnZDDFS2ZTKHCqb4PwbI9zgWyjdHnvci=> NUXlVY9bOTl7Nkm0OlU>
A431 NXvrWFBnU2mwYYPlJGF{e2G7 M2nENVExOCEQvF2= MnjaNVAhdWmw NH\rNnhRem:ub37n[YQhcW6qaXLpeI9zgSCjY4Tpeol1gSCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? M3vTV|E6QTZ7NE[1
Sf9 NHrZ[4tMcW6jc3WgRZN{[Xl? MnrsNVAhdWmw MmrsSG1UVw>? M13RWGlvcGmkaYTpc44hd2ZiRVfGVkBmgHC{ZYPz[YQh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBz\WOncITvdkBifXSxcHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCwMlA{OyEQvF2= MYSyNFA2PjR{NR?=
NCI-H1975 NFjwZ4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVO1JO69VQ>? MV63NkBp NU\Rd4JWUUN3ME24MlI3KM7:TR?= M{\xcVIxOTVzNkew
NCI-H1975 M3XtXWtqdmG|ZTDBd5NigQ>? NFfBeI4yOCEQvF2= NUX3[VVuUW6qaXLpeIlwdiCxZjDFS2ZTKGG3dH;wbI9{eGixconsZZRqd25? NYXl[WZNOjBzNUG2O|A>
NCI-H1975 M3TQOmZ2dmO2aX;uJGF{e2G7 MonLNVAh|ryP NHzmcW9KdmirYnn0bY9vKG:oIFXSRmIzKGG3dH;wbI9{eGixconsZZRqd25? MmrnNlAyPTF4N{C=
NCI-H1975 NVv2WVFjTnWwY4Tpc44hSXO|YYm= M2LWXVUh|ryP MoSzOFghcA>? M3:zUWlv\HWldHnvckBw\iCjcH;weI9{cXNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iClYYPwZZNmNTN? NXjWVFc6OjBzNUG2O|A>
A375 MoTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXBOFghcA>? M121S2lEPTB;MkCuOVUh|ryP MoPKNlA{ODR3M{e=
SMMC7721 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPvNm12PDhiaB?= NVfpO4RmUUN3ME2zN{4yOiEQvF2= MYWyNFMxPDV|Nx?=
DU145 NFnkXZZEgXSxdH;4bYMhSXO|YYm= MnzjOUBl NIrvdJNFVVOR M1L4cmlEPTB;NEKuNVch|ryP MmPRNlA1PjZ3NUW=
MIAPaCa2 NELYZmtEgXSxdH;4bYMhSXO|YYm= NWPONmd{PSCm MWfEUXNQ NVnwS5hDUUN3ME21NE43OiEQvF2= M3GzcVIxPDZ4NUW1
PANC1 MX;DfZRwfG:6aXOgRZN{[Xl? NUThSYNtPSCm NXq5Vpg4TE2VTx?= MV;JR|UxRTRyLkSyJO69VQ>? MVKyNFQ3PjV3NR?=
PC9 M{X1UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkSyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFS2ZTNWSnZnnjbYVvfCCqdX3hckBRSzliY3XscJMhf2m2aDDFR|UxKG:oIECuNFE4KM7:TR?= NWn1V45tOjF{MEi4NFI>
PC9 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jtW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[5Jwf3SqLYLld4l{fGGwdDDoeY1idiCSQ{mgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEV5NE\fRVc2OC:WN{mwUUBufXSjboSge4l1cCCHQ{WwJI9nKDdizszN MX6yNVIxQDhyMh?=
PC3 M{fUWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HIOFExKM7:TR?= MV63NkBp MlLESG1UVw>? NVyzW4lpUUN3ME23MlQh|ryP M2LVT|IyOzV|NUS2
MDA-MB-468 MnPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DWR|ExOCEQvF2= Mkj2OFghcA>? MVjEUXNQ MonyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdIm= NFe3TpgzOTR{OU[zNi=>
Plasmodium falciparum NUDKRZJUeUiWUzDwdo9ncWyrbneg[o9zKGmwaHnibZRwenNib3[gVIxie22xZHn1cUBn[WylaYDhdpVuKHC{b3zp[oVz[XSrb36= Mn61NlE5OTdyNEW=
NCI-H1993 Ml3qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHIcJh1PzJiaB?= MVrEUXNQ MoiyTWM2OD1yLkGg{txO NXXJXY1UOjF7MU[0N|M>
NCI-H292 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLiUWFXPzJiaB?= NV3UbpMzTE2VTx?= NVuydXRTUUN3ME2wMlAzKM7:TR?= NIjK[YkzOTlzNkSzNy=>
A431 M2HESGtqdmG|ZTDBd5NigQ>? MkDGNkBp NIfhcnhFVVOR Mn:3TY5pcWKrdHnvckBw\iCHR1\SJJBpd3OyaH;yfYxifGmxbjDpckBGT0Zvc4TpcZVt[XSnZDDoeY1idiCDNEOxJJdqfGhiSVO1NEBw\iByLkCwNUDPxE1? MUSyNlExOTF|Mh?=
MCF7 NHTsVYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moe1NVAxyqEQvHevcWw> MUC0PEBp MoLaSG1UVw>? M2\VdGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJIdmdmVid3n0bEBKSzVyIH;mJFEzNjB3IN88US=> NYLybphzOjJzMUmxN|A>
BT474 NIK1TYlEgXSxdH;4bYMhSXO|YYm= MkXDNVAh|ryP NH:yPZE1QCCq M36zUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iQmS0O|Qh[2WubIO= NUmyOmZ2OjJzM{izNFg>
MCF7 M3j6TGN6fG:2b4jpZ{BCe3OjeR?= MWmxNEDPxE1? NHHWe4M1QCCq M4XxUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iTVPGO{Bk\WyuczDveoVzNWW6cILld5NqdmdiRYLiRlIh[2WubIO= NIi3UG0zOjF|OEOwPC=>
HCC827 MWjLbY5ie2ViQYPzZZk> MUiwMlEzPSEQvF2= NWPXeFAzOjRiaB?= NH7DVm5FVVOR NHHB[2hKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? M2XqO|IzOzN7M{Sy
HCC827 NWX0cXBoU2mwYYPlJGF{e2G7 NInXW4wxNjF{NTFOwG0> M3X5TlI1KGh? MlLqSG1UVw>? M{L6OWlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCHcnugdIhwe3Cqb4L5cIF1cW:w MWKyNlM{QTN2Mh?=
HCC827 NXq4VXlCU2mwYYPlJGF{e2G7 NUHnNZVuOC5zMkWg{txO MkjJNlQhcA>? NWrRN443TE2VTx?= M{XOO2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:w NVHoenp7OjJ|M{mzOFI>
A431 Mn\RT4lv[XOnIFHzd4F6 MnHiNVAxKM7:TR?= MXuzNEBucW5? NXK4XY9NUW6qaXLpeIlwdiCxZjDFS2ZTKGSncnn2[YQh\nKxbTDoeY1idiCDNEOxJINmdGxibXXtZpJidmViYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyb3z5MWdtfS2WeYKgdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAyNjB6IN88US=> NVvEOoRpOjJ4OUi3PFI>
H460 M4rR[mN6fG:2b4jpZ{BCe3OjeR?= Mn25O|IhcA>? MULJR|UxRTVwNUmg{txO MXeyNlczPzR2OR?=
HT-29 MXPDfZRwfG:6aXOgRZN{[Xl? M3njXFczKGh? M3XqWWlEPTB;Mz6zOkDPxE1? NWXkb|ZKOjJ5Mke0OFk>
SGC7901 NFXSelREgXSxdH;4bYMhSXO|YYm= M2WxOFczKGh? MV;JR|UxRTFyLkK2JO69VQ>? NFX2PXUzOjd{N{S0PS=>
A498 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvlTIN{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFk5KGOnbHzzJIV5eHKnc4PpcochTUeIUjDhcoQhW1KFIIfpeIghUUN3MDDv[kA5NjZizszN NXPMNpRlOjJ6MUi4OFg>
Calu3 NXSxcXF{S3m2b4TvfIlkKEG|c3H5 NFHa[4wyOCEQvHevcWw> NE\tfos4OiCq MmPNTWM2OD1zLk[g{txO NVq3eXI5OjNzMU[xOlg>
FADU Mle4R5l1d3SxeHnjJGF{e2G7 NWLiWG1vOTBizsznM41N M2HiUFczKGh? MVfJR|UxRTBwMEWg{txO Ml3xNlMyOTZzNki=
HCC827 MVPDfZRwfG:6aXOgRZN{[Xl? NFzv[GEyOCEQvHevcWw> NWHM[lNXPzJiaB?= MXnJR|UxRTBwMECxOUDPxE1? NWrzW4NPOjNzMU[xOlg>
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IGROV-1 MmrpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHweHVZUUN3ME2yMlMyOjN5IN88US=> NEW5T2NUSU6JRWK=
JVM-3 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TMZmlEPTB;Mj6zOFY1OyEQvF2= MoDzV2FPT0WU
NCI-H2170 NWTvfHhFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLDTWJuUUN3ME2yMlM1Pzh|IN88US=> M1rnV3NCVkeHUh?=
EW-18 NYXlNFRQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XW[mlEPTB;Mj6zOVQ4OSEQvF2= NY\RdIdUW0GQR1XS
KU812 MnzUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTJwM{[3O|kh|ryP NH;HbYxUSU6JRWK=
NY MnzkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3xWHN7UUN3ME2yMlM4OjdizszN M3PyVnNCVkeHUh?=
HTC-C3 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NInNTnBKSzVyPUKuOFIzPjdizszN M2PjSHNCVkeHUh?=
CFPAC-1 NFLST2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDZV3VKSzVyPUKuOFM5PjRizszN NHf0e4NUSU6JRWK=
HH NHfXcG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrzUWpCUUN3ME2yMlQ6QDV2IN88US=> NGXLT5BUSU6JRWK=
MKN7 NX;mdHdRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPNTWM2OD1{Lk[yO|Q5KM7:TR?= NHTNW5pUSU6JRWK=
TE-8 Mn:4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTJwNkO2OVMh|ryP NFjoOYdUSU6JRWK=
MC-IXC Ml\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXCTWM2OD1{Lk[0OFU{KM7:TR?= Ml\GV2FPT0WU
DMS-79 NX7NeZc4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXTcpRKSzVyPUKuOlkzQDRizszN NGL3SHpUSU6JRWK=
Detroit562 MnXMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mkj0TWM2OD1{Lk[5PFQ2KM7:TR?= M4GwN3NCVkeHUh?=
HuP-T4 MlPZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;QbVlKSzVyPUKuO|I5OThizszN NV\tTXR4W0GQR1XS
QIMR-WIL MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHz0OGlKSzVyPUKuO|U6PTJizszN M1myOHNCVkeHUh?=
BB65-RCC MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTJwOEC3NVMh|ryP Mon2V2FPT0WU
PC-3 MkL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXkfGZ3UUN3ME2yMlg{OzF5IN88US=> NVzpVWxxW0GQR1XS
OVCAR-3 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTJwOEW1PVQh|ryP M3z4TnNCVkeHUh?=
KYSE-510 NX:zRXA4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTJwOE[3PFYh|ryP MXrTRW5ITVJ?
HC-1 NVTR[5JRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLjTWM2OD1{Lkm0NFEzKM7:TR?= MlfKV2FPT0WU
NCI-H1693 M3uzdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnOxTWM2OD1{Lkm1PFk4KM7:TR?= M1vxb3NCVkeHUh?=
DU-4475 MknFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTJwOUmwOVIh|ryP MlHCV2FPT0WU
COLO-680N MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTNwMEC0PVUh|ryP MkizV2FPT0WU
MN-60 M2TyPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoftTWM2OD1|LkC0PVc1KM7:TR?= MmfjV2FPT0WU
BCPAP NGLLOYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDHTWM2OD1|LkC4Nlk2KM7:TR?= NX7POWdTW0GQR1XS
647-V MlK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTNwMU[yNkDPxE1? NITsXWdUSU6JRWK=
HT-1376 NHzTOVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2G3[GlEPTB;Mz6yNFUxPiEQvF2= NWfIT3FsW0GQR1XS
NCI-H1793 NVPVWHl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmn5TWM2OD1|LkKxNFk5KM7:TR?= MlLoV2FPT0WU
SW1463 NF;w[HBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVr6O5hFUUN3ME2zMlI3OTl{IN88US=> NFPxbYNUSU6JRWK=
NCI-H727 M4D5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTNwM{i1PFQh|ryP NVu2SYxYW0GQR1XS
LB771-HNC MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnhTWM2OD1|LkO5NVI3KM7:TR?= Mom4V2FPT0WU
D-542MG M4rCU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvFfYFKSzVyPUOuOFQzOjJizszN MUHTRW5ITVJ?
NTERA-S-cl-D1 MnXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTFfHRKSzVyPUOuOFQ6PTdizszN Mn3HV2FPT0WU
C8166 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrNVpcyUUN3ME2zMlQ3PTJzIN88US=> NEHxWHFUSU6JRWK=
639-V MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HmeWlEPTB;Mz61O|c6OiEQvF2= MVrTRW5ITVJ?
TE-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPxb|ByUUN3ME2zMlU6OTZ7IN88US=> M2DYcXNCVkeHUh?=
OAW-28 NF;YfppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTjWYZKSzVyPUOuOVk1OjZizszN MUPTRW5ITVJ?
SK-LU-1 M37YNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTNwNkC2OFUh|ryP NHz6ZnNUSU6JRWK=
CaR-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTNwNkGwOFgh|ryP M3X3XnNCVkeHUh?=
A2780 NWDiXFlmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVz5c4ZMUUN3ME2zMlY1ODh7IN88US=> NXjDNmZsW0GQR1XS
C2BBe1 NFrWUmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfveohqUUN3ME2zMlY1PDB5IN88US=> MVXTRW5ITVJ?
SK-MES-1 MoC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnGTWM2OD1|Lk[0OlE1KM7:TR?= NYSyWYdbW0GQR1XS
PANC-10-05 NHTwPJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NES3eoFKSzVyPUOuO|IxPzJizszN NH[wTWpUSU6JRWK=
J-RT3-T3-5 NVXpWJlTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWO1UXIyUUN3ME2zMlc2PDB4IN88US=> M1[5dHNCVkeHUh?=
ES4 NUDIOpJST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTNwOEKyPFYh|ryP MljHV2FPT0WU
NCI-H28 NFz6eVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PxRmlEPTB;Mz64Nlk3OSEQvF2= NFf3c5lUSU6JRWK=
NCI-H1155 M2TFOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TzdGlEPTB;Mz64OFE2QSEQvF2= NX\t[|d7W0GQR1XS
KURAMOCHI M3vycmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nVVmlEPTB;Mz64OlA2PiEQvF2= MVvTRW5ITVJ?
NCI-H69 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fLSGlEPTB;Mz65NFE4QCEQvF2= MWHTRW5ITVJ?
IST-MES1 MmXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTNwOUSwNlkh|ryP MkfSV2FPT0WU
NH-12 MknWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnHUopKSzVyPUOuPVQyQDdizszN MVXTRW5ITVJ?
JVM-2 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofDTWM2OD1|Lkm0O|UzKM7:TR?= M1uyW3NCVkeHUh?=
EW-16 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfXWlBVUUN3ME2zMlk2PTd5IN88US=> MXnTRW5ITVJ?
NCI-H441 NW\lclhbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTNwOUewNVQh|ryP MYDTRW5ITVJ?
RCC10RGB NVrlSIZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2X4RmlEPTB;ND6wNVI1PiEQvF2= M4myRnNCVkeHUh?=
TCCSUP M2nMfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1H0fGlEPTB;ND6xNVA2KM7:TR?= M2j4S3NCVkeHUh?=
HL-60 MoPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TNVmlEPTB;ND6xNlI1OSEQvF2= NH7qe5lUSU6JRWK=
EW-11 NVLYe|dIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4SwPWlEPTB;ND6xN|I3OSEQvF2= MoDzV2FPT0WU
KARPAS-299 NGSwd5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTRwM{GwNVMh|ryP NGWxcpNUSU6JRWK=
SNU-C2B Mn3qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDtVXNYUUN3ME20MlM1Pjh4IN88US=> NWfWRpZoW0GQR1XS
LU-139 M1PFVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX32ZXAxUUN3ME20MlM4OjR5IN88US=> NV;lRlk2W0GQR1XS
HCC1937 MlT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzSUFJ3UUN3ME20MlM4QTl|IN88US=> MVzTRW5ITVJ?
GR-ST MnKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTRwNEOxPEDPxE1? NET0XWVUSU6JRWK=
SK-OV-3 MkDnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Moq3TWM2OD12LkS2NFQzKM7:TR?= M2XLenNCVkeHUh?=
A172 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTRwNUW1NlEh|ryP MlLzV2FPT0WU
NB10 MnP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfDTWtUUUN3ME20MlU6PzhzIN88US=> NFjr[5pUSU6JRWK=
P30-OHK MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vy[2lEPTB;ND64NVkzQSEQvF2= M{XhfHNCVkeHUh?=
KYSE-520 M{LQVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHex[2FKSzVyPUSuPFgxOThizszN NYrHRZM{W0GQR1XS
KNS-62 NETnRZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTDeJFiUUN3ME20MlkxQDB5IN88US=> Mk\vV2FPT0WU
ES1 M{HJfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\zTWM2OD12Lkm2NFM2KM7:TR?= NXvwWVBGW0GQR1XS
KOSC-2 M2DT[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4K5bWlEPTB;ND65PVk2QSEQvF2= MX;TRW5ITVJ?
TE-9 NGC5[YxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTVwMEG1NlUh|ryP NYXNOnIxW0GQR1XS
NCI-H1838 NY\rcXB7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\tTWM2OD13LkCyNFc3KM7:TR?= MYnTRW5ITVJ?
KP-N-YN MkLnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jvOWlEPTB;NT6xNlU6OSEQvF2= MVHTRW5ITVJ?
ALL-PO M3rxeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHv3[WdKSzVyPUWuNlI2QDdizszN NEPZOFBUSU6JRWK=
NOMO-1 NGjWUYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVq2Z|FiUUN3ME21MlI{OTd4IN88US=> MkL6V2FPT0WU
CGTH-W-1 NH21e41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTVwM{i1PFQh|ryP NFTvVHBUSU6JRWK=
ATN-1 NF\wbXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;KWGlEPTB;NT60NVE5PCEQvF2= NWXGW2xVW0GQR1XS
AGS MmLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDaeWFVUUN3ME21MlQ6PjF6IN88US=> NXnENpRzW0GQR1XS
SW13 NIX5WFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nyO2lEPTB;NT61N|I3PSEQvF2= NHfueFBUSU6JRWK=
SW1990 NVH5SWttT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rrd2lEPTB;NT62NlI5PSEQvF2= MXzTRW5ITVJ?
BFTC-905 NULnSnB6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTVwNkW4O|gh|ryP NXfCWYcxW0GQR1XS
RMG-I NXi1fnNYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HlemlEPTB;NT62OVk1OiEQvF2= M2\2OXNCVkeHUh?=
HD-MY-Z NXnMXm82T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1qweGlEPTB;NT62PFU5OSEQvF2= NFvwTFFUSU6JRWK=
HCC1187 M4n5dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUK3blRlUUN3ME21MlcyODN{IN88US=> M3TOT3NCVkeHUh?=
HCC2998 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXrcJFKSzVyPUWuO|E5PzdizszN NYnLU4w{W0GQR1XS
RT-112 MlHVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTVwOUCyOFUh|ryP MUjTRW5ITVJ?
CAKI-1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3ryXGlEPTB;Nj6wNFk5PSEQvF2= M2DFOHNCVkeHUh?=
HDLM-2 M1TPNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrScI83UUN3ME22MlAyPDh7IN88US=> MlPDV2FPT0WU
TE-10 NUi0NXNKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4X2XGlEPTB;Nj6wOFg{OyEQvF2= MUHTRW5ITVJ?
KE-37 NHTZXpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoO4TWM2OD14LkC3NlU5KM7:TR?= NF7leW5USU6JRWK=
RCM-1 NImxXIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTZwMEe3NVkh|ryP NGPtXVdUSU6JRWK=
Mewo NEX0T2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTZwMUG0PFch|ryP MkSxV2FPT0WU
L-428 NE\V[25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrpTWdoUUN3ME22MlE5ODN5IN88US=> NInGfGxUSU6JRWK=
8-MG-BA M1zsZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnpSpFKSzVyPU[uNVk{PzlizszN NWXMR3c5W0GQR1XS
SK-MEL-28 M1fiSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rOVWlEPTB;Nj6yNFk3PyEQvF2= M1zKenNCVkeHUh?=
VA-ES-BJ MlTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTZwM{C0OlIh|ryP M161OHNCVkeHUh?=
CAL-120 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLUTWM2OD14LkS1NVYzKM7:TR?= NYDiPGhuW0GQR1XS
GAMG Mmr5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjTR2VKSzVyPU[uOVA3OiEQvF2= M2raU3NCVkeHUh?=
MSTO-221H MkGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTZwNUGxO{DPxE1? MoXMV2FPT0WU
GCT NV7Rc295T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1m3fWlEPTB;Nj61N|M5PyEQvF2= MkjpV2FPT0WU
SH-4 MkCxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Moi4TWM2OD14LkW4PFQ1KM7:TR?= NGjsRplUSU6JRWK=
Calu-3 NWXFWpY{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTZwNkW1Olgh|ryP NG[zXHJUSU6JRWK=
SK-HEP-1 NXvCSmxTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XNbWlEPTB;Nj63NVc2OyEQvF2= MYrTRW5ITVJ?
PFSK-1 NH[4e|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnMOHRKSzVyPU[uO|M1PzhizszN M2rYT3NCVkeHUh?=
NB12 Mn\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTZwN{i0NFQh|ryP MoHSV2FPT0WU
A388 NILyfVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\JTWM2OD14Lki5OVIh|ryP MoDJV2FPT0WU
KLE NXnYTo86T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWP5R3JsUUN3ME22Mlk3PTF4IN88US=> NX3sfIpQW0GQR1XS
TE-11 M2nrfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTdwMES5Olch|ryP MUDTRW5ITVJ?
NCI-H2405 M1zrZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXqb4VNUUN3ME23MlA5Ozl|IN88US=> NUHCO|Z{W0GQR1XS
OVCAR-4 M2CwXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUWxRVNRUUN3ME23MlE2OzVizszN MlW5V2FPT0WU
KP-N-YS MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnH5TWM2OD15LkG4NVQh|ryP NHPFUVhUSU6JRWK=
NOS-1 M4TJXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XxW2lEPTB;Nz6yOVgzQCEQvF2= NGfSPXVUSU6JRWK=
DMS-114 Mk\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTdwMkm2N|Eh|ryP NIXUbYNUSU6JRWK=
NCI-H2030 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTdwM{O2PVgh|ryP M12yeXNCVkeHUh?=
HEL MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTdwM{e1NlEh|ryP MYjTRW5ITVJ?
CAL-62 MkL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4C0dmlEPTB;Nz6zPVg3PiEQvF2= NGLSTJFUSU6JRWK=
COLO-668 NEXDUGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXPTWM2OD15LkSzPVY4KM7:TR?= MWrTRW5ITVJ?
SKG-IIIa MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorYTWM2OD15LkS1N|gyKM7:TR?= MWPTRW5ITVJ?
HCC1419 NUPS[mJKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTdwNE[4N|Mh|ryP NVvOSINYW0GQR1XS
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COLO-829 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHqbFVKSzVyPUeuOlY1PjNizszN M3K5cnNCVkeHUh?=
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NB14 NHG3WnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;BN2lEPTB;M{iuOFU1PCEQvF2= NGq0foRUSU6JRWK=
D-423MG MlzmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3ZcnlKSzVyPUO4MlQ5PjlizszN NV3FNGM6W0GQR1XS
NCI-H510A MkHlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLyXmxKSzVyPUO4MlY1PTFizszN MYrTRW5ITVJ?
NMC-G1 Mln3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXZXFdKSzVyPUO4MlczOjJizszN NX3WRpBlW0GQR1XS
SW948 M4fFbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\mfmZKSzVyPUO4Mlg6PDlizszN M3LoRXNCVkeHUh?=
MFM-223 M1rhdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIO3V5JKSzVyPUO4Mlk1OzFizszN M2\IRXNCVkeHUh?=
PAN1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\sTWM2OD1|OD65PVE6KM7:TR?= NI\hSndUSU6JRWK=
MZ7-mel MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fRZmlEPTB;M{muNVE6QSEQvF2= M2nCdHNCVkeHUh?=
COLO-679 NVzXcFd7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTkTWM2OD1|OT65O|A5KM7:TR?= Ml;tV2FPT0WU
SW872 NVfkR2U5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTRyLkKwN|Eh|ryP NGXRVmRUSU6JRWK=
HT-1197 NWPzXWNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4eweGlEPTB;NECuNlg3QSEQvF2= M3K0[3NCVkeHUh?=
ES6 NIjHcpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTRyLkS1OVIh|ryP MVPTRW5ITVJ?
SNU-387 M{PUTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{S0WmlEPTB;NECuPFI{QCEQvF2= MlLkV2FPT0WU
T47D NHvqe3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DRSmlEPTB;NEGuNFE6OiEQvF2= M{[zdHNCVkeHUh?=
G-402 MmDyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXITWM2OD12MT6wPFA6KM7:TR?= NHrYdnhUSU6JRWK=
MZ1-PC NV[xZXhJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfITWM2OD12MT64NUDPxE1? NH3ifoFUSU6JRWK=
SW1417 MmrWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LHfmlEPTB;NEKuN|A{KM7:TR?= MYnTRW5ITVJ?
RERF-LC-MS NGDZ[WxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPaTWM2OD12Mj61NFg4KM7:TR?= NELYdmpUSU6JRWK=
SF539 NHvXUW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTR{LkW1Nlkh|ryP NVLUdY93W0GQR1XS
ESS-1 NYHYdIFtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVOwWpprUUN3ME20N{42PTV6IN88US=> NXzRNoVoW0GQR1XS
RO82-W-1 MmrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3L2[2lEPTB;NEOuOlE2OyEQvF2= MYnTRW5ITVJ?
S-117 NUK5[|VHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XvOGlEPTB;NEOuOlk2QCEQvF2= M4fkd3NCVkeHUh?=
SW756 Mn[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTR|LkiyOFIh|ryP NFjuRVBUSU6JRWK=
CP66-MEL NH3IdYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvyTWM2OD12NT63NVQ{KM7:TR?= MnXzV2FPT0WU
H-EMC-SS MlTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTR3Lke2PVMh|ryP MojWV2FPT0WU
NCI-H2052 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XIcWlEPTB;NEWuPVAzPyEQvF2= NF3PNWpUSU6JRWK=
SW684 NIjhNnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfIfoJKSzVyPUS3MlAxQDFizszN MkG0V2FPT0WU
HCC1569 NYTUSGhHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTnZZlxUUN3ME20O{4yPjV6IN88US=> MV;TRW5ITVJ?
KGN NGHtepZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nofGlEPTB;NEeuN|MyOSEQvF2= NUTVOmxmW0GQR1XS
LCLC-103H NInYRW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXMenlKSzVyPUS3MlQ1ODRizszN NED4N4ZUSU6JRWK=
SW780 NWro[lBmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvYTWM2OD12Nz62N|UyKM7:TR?= NYPBe5A6W0GQR1XS
NCI-H1703 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFSwRmtKSzVyPUS4MlM{PTJizszN MY\TRW5ITVJ?
HCC1395 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTR6Lke0N|Ih|ryP NFTreGhUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo試験 Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

プロトコル(参考用のみ)

細胞アッセイ: [1]
+ 展開
  • 細胞株: NR6, NR6M and NR6W cells
  • 濃度: 0-2 μM
  • 反応時間: 72 hours
  • 実験の流れ: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (参考用のみ)
動物実験:[3]
+ 展開
  • 動物モデル: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • 製剤: 0.5% polysorbate
  • 投薬量: 100 mg/kg
  • 投与方法: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water <1 mg/mL
体内 5% DMSO+corn oil 2.5mg/mL

* <1 mg/mlは製品が微弱に溶解する或いは溶解しないことを示します。
* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 446.90
化学式

C22H24ClFN4O3

CAS No. 184475-35-2
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

臨床試験

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

EGFR信号経路図

EGFR Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID