Trichostatin A (TSA) 化学構造
分子量: 302.4

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製品説明

  • Compare HDAC Inhibitors
    HDAC製品生物活性の比較
  • 研究分野
  • Combination Therapy
    併用療法
  • Trichostatin A (TSA)のメカニズム

製品の説明

生物活性

製品説明 Trichostatin A (TSA) は、HDAC阻害剤で、IC50 が ~1.8 nM。
ターゲット

HDAC

IC50

~1.8 nM [1]

In vitro試験 Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
HEK 293 NVO3OHI1TnWwY4Tpc44hSXO|YYm= MYGwMlfPxE1? MmjBNlRp M3fLWIV1cGGwb3y= NEj3XpZmdmijbnPld{BGVmGFIHHj[ZR6dGG2aX;uJIFv\CCrbnPy[YF{\XNiRV7hR{Bi[nWwZHHuZ4UhcW5idHjlJJRwfGGuIHPlcIwhdHm|YYTlJIFv\CCjdDD0bIUh[2WubDDzeZJn[WOn MVSyOVc5PzB5OR?=
TE13 Ml3tSpVv[3Srb36gRZN{[Xl? MWmwMlPPxE1? M4HlTlI1cA>? NIfCRmh2eC2{ZXf1cIF1\XNiUlHTV2Y2SSCuZY\lcC=> MkG3NlU2Pzl4NkW=
TE13 M3HicGFxd3C2b4Ppd{BCe3OjeR?= MVuwMlPPxE1? MUCyOIg> MYnpcohq[mm2czD0bIUh[2WubDDwdo9tcW[ncnH0bY9v NE\lZ4kzPTV5OU[2OS=>
MEFs MnS1SpVv[3Srb36gRZN{[Xl? NWX6[npuPc7:TR?= NHS1flIyPmh? M370OIlv[3KnYYPld{B1cGViRWDFR{BifHSjY3jt[Y51NCCWaYKg[IVtcX[ncomgZY5lKHSqZTDl[oZq[2mnbnP5JI9nKHCnZHXzeIFtKG[xcn3heIlwdg>? Ml7CNlU1QDJ4M{S=
SW480  MYfGeY5kfGmxbjDBd5NigQ>? NFrLdogxNjIQvF2= MnTlOFhp Mn7kSG1UVw>? M2D1bZJmfmW{c3XzJGVOXA>? NXuwfGppOjV2M{S5PVc>
PC3  NIG1bGNHfW6ldHnvckBCe3OjeR?= MWCwMlHPxE1? MVW0PIg> MXfEUXNQ NVjKbJl2emW4ZYLz[ZMhTU2W NGnZe3MzPTR|NEm5Oy=>
SW480  M4\icmZ2dmO2aX;uJGF{e2G7 MnjQNE4y|ryP NITYU5Q1QGh? MYTEUXNQ MX\heJRmdnWjdHXzJIlvfmG|aX;uJIFv\CCvaXfyZZRqd25? M4DwXlI2PDN2OUm3
PC3  NYfs[|RbTnWwY4Tpc44hSXO|YYm= NVPRT41ZOC5zzszN M4LDRVQ5cA>? NHnZW5FFVVOR MUDheJRmdnWjdHXzJIlvfmG|aX;uJIFv\CCvaXfyZZRqd25? M4PYRVI2PDN2OUm3
SW480  NUf1bndTTnWwY4Tpc44hSXO|YYm= NELTRXgxNjIQvF2= M4fze|Q5cA>? NXiyeo5STE2VTx?= MmjNbY5lfWOnczDpcoNz\WG|ZTDv[kBJTEGFMTDhcoQhUESDQ{Kgc44hW2y3ZzDn[Y5meyCycn;tc5Rmeg>? MkWwNlU1OzR7OUe=
PC3  NIKxPFZHfW6ldHnvckBCe3OjeR?= M{e1WVAvOc7:TR?= M1j4R|Q5cA>? MU\EUXNQ NHnHblRqdmS3Y3XzJIlv[3KnYYPlJI9nKEiGQVOxJIFv\CCKRFHDNkBwdiCVbIXnJIdmdmW|IIDyc41wfGW{ NIf2OGQzPTR|NEm5Oy=>
A431 M3PGdWFxd3C2b4Ppd{BCe3OjeR?= M{PLXFIwOTBxNUCvNVAxdk1? M1vyNlQ5cA>? MYXEUXNQ MnTYbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3Sq Ml7qNlU{PzFyNkm=
A431 NIXKTnpHfW6ldHnvckBCe3OjeR?= NHHDNoM2OG6P MkThNk83NzF{L{K0bC=> M2G3W2ROW09? Mnj2ZYN1cX[jdHXzJJAzOSCjbnSgbY5pcWKrdIOgRXRHOyCneIDy[ZN{cW:w MViyOVM4OTB4OR?=
MDA-MB-231 NGTXPXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlP0NE03ODCwTR?= MoDjNlRp MoHGSG1UVw>? NG\jZlRKSzVyIH;mJFExOG6P MoHSNlUyQTJ5MkG=
MCF7 M1;wZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHH6dYQxNTZyMH7N NX73UIxZOjSq NFPudFhFVVOR NXLFWIxXUUN3MDDv[kA4PW6P M1WySVI2OTl{N{Kx
SKOV-3 NUnRSlNCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3vNU0yOM7:TR?= MXOyOIg> MmLSSG1UVw>? NXrNSXRzUUN3MDDv[kA2NjcQvF2= NXy2dFVZOjVzNkm0PVE>
A549 NGqwUVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TYS|EuOTEQvF2= NHT4SnYzPGh? MVPEUXNQ NXG5cXd1UUN3MDDv[kA{NjMQvF2= M3\1clI2OTZ7NEmx
SKOV-3 M2LTW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUOwMlEuOc7:TR?= NWjkS3BwPDiq NH;PW3JFVVOR M4Swd2lEPTBib3[gNE44|ryP NGXofVUzPTF4OUS5NS=>
A549 NX3HR|l1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXVU20xNjFvMd88US=> MljvOFhp MkT0SG1UVw>? MYjJR|UxKG:oIECuNljPxE1? Ml7qNlUyPjl2OUG=
SKOV-3 NUPtSnA4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTWXmoxNjBzLUCuPe69VQ>? MnrJO|Jp NWjzPYNtTE2VTx?= M3LOfGlEPTBib3[gNE4{Os7:TR?= NX7CV3VDOjVzNkm0PVE>
A549 NFfDdYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TwWVAvODFvMD65{txO M{mz[|czcA>? NWLnT|JQTE2VTx?= NH\zZ2FKSzVyIH;mJFAvODcQvF2= NGTBPFUzPTF4OUS5NS=>
HeLa NVvid4hHTnWwY4Tpc44hSXO|YYm= NHPKXW8zPTCwTR?= M{nSfVE3cA>? Mo\GSG1UVw>? NFLwdZdqdmO{ZXHz[ZMhS1mSMVGxJI1TVkFiZYjwdoV{e2mxbtMg NELEZYczPTFzNk[4PC=>
CNE2 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzQNVAxNTZyMH7N MVOyOE81QC95Mni= MnfvbY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gbY4h[SC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MVyyOFk3QTlyMR?=
PC3 NILLOodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mke1NVAxNTFyMEDuUS=> MkG4NlRp NI[5TWpKSzVyIH;mJFMxOG6P MnHUNlQ5PTR4NUi=
LNCaP NHn6RmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLpU2ptOTByLUGwNFBvVQ>? NGPWfZIzPGh? MYHJR|UxKG:oIEOwNI5O MmLBNlQ5PTR4NUi=
HeLa  NVrRRWQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTUfnMz|ryP MYi0PIg> MVjk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliYXLveZQhOjVn NIPsZWgzPDh2NkGzOS=>
HMEC-1  MVrGeY5kfGmxbjDBd5NigQ>? MVSzNFBvVQ>? MWmyOIg> NUHmc2M6cW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>? M3jKdVI1PzFyNkOx
HeLa MWHGeY5kfGmxbjDBd5NigQ>? NYe4RZFiOW2P NWXWfmhYOC53aB?= MU\hZo9tcXOqZYOgeIhmKHSxdHHsJGhFSUNiYXP0bZZqfHl? M3jYNVI1PzB5NEe0
ACP02 NED0eVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4nsbFI2OC9|NUCvOVAxdk1? NITSV4QzPGh? MonN[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>? MlHoNlQ3Pjh3NEe=
ACP03 NGHYepZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTafHFYOjVyL{O1NE82ODCwTR?= M2\mfVI1cA>? M3yyXoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDv[kBieHC{b4jpcYF1\Wy7IEewxsAmKGG2IEK1NOKhdk1? M3rndlI1PjZ6NUS3
U87 GBM NFj3T4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1i4UlExOC9|MECvOVAxKG6P M4e2TFczKGh? NGDLPHUyODBnIHX0bIFvd2x? MkjJdoVlfWOnczDt[YFvKGOnbHygcpVu[mW{IHL5JFMyNCB3NDygZY5lKDV6wrCl NYLtc5lMOjR2NkS4OFE>
U87 GBM MUHGeY5kfGmxbjDBd5NigQ>? MnjjNVAxNzVyMDDOcS=> Ml7xOFghcA>? MnroNVAxLSCndHjhco9t NYnjU21NUW6mdXPld{BU\W6nc3PlcoNmNUyra3WgRYx1\XKjdHnvcpMhcW5iToXjcIVieiCPb4LwbI9td2e7 M4DpNVI1PDZ2OESx
RPE Mlr5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfJclMxNjJxMD60M|AvQC9zIN88US=> M2e4[|I1NzR6L{eyJIg> MW\EUXNQ MlG2bY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gZpkh[2WubDDjfYNt\SCjcoLld5Q> Mn\5NlQ1PTZ4MEK=
HT29  Mnf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXqxPFAhdk1? NVTofFNZPzJiaB?= NG\BS2NKSzVyIH;mJFE5OCCwTR?= M1K2dVI1OzZ6Mk[1
hMSCs NGjPUXdHfW6ldHnvckBCe3OjeR?= M{jMS|YvOjVibl2= Ml\wNlQhcA>? NXe3e3RZTE2VTx?= MYrzeIFjcWyrenXzJGhqe3SxbnWgRYNmfHmuYYTpc44h[W6mIITo[UBGgHC{ZYPzbY9vKG:oIGDseZJqeG:2ZX70JGdmdmW| NEHiRXozPDNzMkO1Oi=>
Huh7  MoDOSpVv[3Srb36gRZN{[Xl? NILTbIQxNjFxMD61M|EvOCEQvF2= NHvmNpozPGh? NYjmUoNVe3WycILld5NmeyC\VFjER|Ih\2WwZTDlfJBz\XO|aX;u MYeyOFI3QTZ5Mh?=
SKOV3 NGrXTG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MliyNE4xPS1{IN88US=> Mk\ZNVAwOjRxNEigbC=> M3zwcGROW09? NV[zb|NndWWmaXH0[ZMh\3Kxd4ToJIFzemW|dDDpckBiKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= MmfnNlQzOjN6MEG=
A2780 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvrS5JOOC5yNT2yJO69VQ>? NH3QfZQyOC9{ND:0PEBp MnfBSG1UVw>? NHnBd|hu\WSrYYTld{Boem:5dHigZZJz\XO2IHnuJIEh[2:wY3XueJJifGmxbj2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= M2W2SFI1OjJ|OECx
SRA01/04  MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEL0cYExNjJizszN M2LBUVQ5KGh? NF;4N2xFVVOR M2jXWZN2eHC{ZYPz[ZMh[2WubDDwdo9tcW[ncnH0bY9vKHSqcn;1[4ghe3WycILld5NqdmdidHjlJHBKO0txQXv0MEBxOziPQWDLJIFv\CCHUluxM|Ihe2mpbnHsbY5oKHCjdHj3ZZl{ NETTcY0zPDF3N{i3PC=>
HLEB3 M4rIeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoC0NE4zKM7:TR?= M{TYSFQ5KGh? M33i[WROW09? NVPDSWJ6e3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC|dYDwdoV{e2mwZzD0bIUhWEl|Sz;Bb5QtKHB|OF3BVGsh[W6mIFXST|EwOiC|aXfuZYxqdmdicHH0bJdigXN? MYCyOFE2Pzh5OB?=
SRA01/04  M1\pXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWqwMlQwOC56IN88US=> NHK2d3M1QCCq NHrvfoxFVVOR NXHGfHF[cW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= M3nIW|I1OTV5OEe4
HLEB3 MnPCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfOfYkxNjRxMD64JO69VQ>? MkjtOFghcA>? M{DXRmROW09? MYnpcoR2[2W|IHPlcIwh[XCxcITvd4l{ MmLTNlQyPTd6N{i=
HCT116 M4DZU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M125ZVAvOiEQvF2= MXexNkBp M1nyWoVvcGGwY3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgdoFlcWG2aX;u NH\PWHkzPDF{MkKzNS=>
CA46 M4j6bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzac5c{NzZxMUKvNlQwPDhibl2= M4jGOlQ5KGh? MmDqbY5pcWKrdIOgeIhmKGOnbHygdJJwdGmoZYLheIlwdiCjdDCyOEBidmRiNEigcm3DqA>? MXqyOFA3PDl3MR?=
PMNs MmnvSpVv[3Srb36gRZN{[Xl? NFXGbG0{OCCwTR?= NXPUemUzPDhiaB?= Mn7PbY5pcWKrdIOg[YZn\XKxY4n0c5NqeyxiSFTBR{Bi[3Srdnn0fUBidmRiUnHjJIFkfGm4aYT5 NHrZOHUzOzl6OE[xOy=>
H1299 Ml;ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvWRocyyqEQvF5CpC=> NVXTZ4VzOjRxNEivO|IhcA>? M3r3RoV1cGGwb3y= MUnpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBi\nSncjCyOIg> M{ThXlI{QTF4NkC5
A549 NVzlflUyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjYd2cxNjJ3L{CuOU8yKM7:TR?= M{nWblI1NzR6L{eyJIg> NGHUe45mfGijbn;s NHjjO25qdmirYnn0d{B1cGViY3XscEBoem:5dHigbY4h[2:wY3XueJJifGmxbjDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NIPhUYozOzh4N{m5NS=>
H1299 M3vS[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWCwMlI2NzBwNT:xJO69VQ>? M4fJNlI1NzR6L{eyJIg> MWTleIhidm:u MUXpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX6gZ49v[2WwdILheIlwdiCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz MVGyN|g3Pzl7MR?=
A549 M2jJSmFxd3C2b4Ppd{BCe3OjeR?= M1v0eFAvPS9zIN88US=> NXiyPJFQPDhiaB?= Mn7q[ZRp[W6xbB?= MoLIbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MYGyN|g3Pzl7MR?=
H1299 MnywRZBweHSxc3nzJGF{e2G7 NX[4UVNZOC53L{Gg{txO NVfQTnhXPDhiaB?= NFjsWVZmfGijbn;s MkXpbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MW[yN|g3Pzl7MR?=
SUM149PT MoDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17ZTVIwPy53L{GwJO69VQ>? NFW4UYI1QCCq MVjEUXNQ NYS3Z|lVcW6mdXPld{Bk\WyuIHTlZZRpKGG2IEKg{txOKGmwc3Xud4l1cX[nbIm= NXWybYt1OjN5OUK2N|g>
SUM190PT NXPaUI5NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUW1NE8yODBxMkWwJI5O NUjIO29[PDhiaB?= M37ZPGROW09? NV;RSXk5cW6mdXPld{Bk\WyuIHTlZZRpKGG2IEWwJI5OKHOnboPpeIl3\Wy7 NF76N5ozOzd7Mk[zPC=>
HCT1 NWjzSFFZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nKWFAvOi9zLkCvOU4xKM7:TR?= NIPLZW0yOi9{ND:zOk81QCCq MWnpcoR2[2W|IHPlcIwh\GWjdHigbY4h[2:wY3XueJJifGmxbj2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= NYrJXHpmOjN5N{CwNFA>
Lovo M2TITGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7DSmxIOC5{L{GuNE82NjBizszN NGHG[3oyOi9{ND:zOk81QCCq M2n5R4lv\HWlZYOgZ4VtdCCmZXH0bEBqdiClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NFvt[JUzOzd5MECwNC=>
AGS NV3qc21ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzNNE4xOTVvMTFOwG0> MkD5O|IhcA>? NVK3VVlLcW6mdXPld{Bk\WyuIHTlZZRpKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NXLyd3MyOjN5NEWwNlQ>
Huh7 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLrNmwyKM7:TR?= NWjodXZWOjRiaB?= MWnEUXNQ NHnIW4xz\WS3Y3XzJIFjd3W2IEKwKUB3cWGkaXzpeJnDqA>? M{PaPVI{PjR|OUOz
ECC1 NEmyOJNHfW6ldHnvckBCe3OjeR?= MV21NFAhdk1? NH3sZXQ2KGR? Mli4SG1UVw>? NV\aXIxLcW6mdXPld{BNOUODTdMg[ZhxemW|c3nvckBkdy22cnXheI1mdnRid3n0bEA2NUG8YVO= M1y1UFI{PTNyN{[5
HEC1A NHuxU2tHfW6ldHnvckBCe3OjeR?= NYrX[llkPTByIH7N NHjpVFg2KGR? NH[0OWJFVVOR M{jldIlv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF NHH3WVUzOzV|MEe2PS=>
EN1 NIPHW|FHfW6ldHnvckBCe3OjeR?= NEez[WY2ODBibl2= NGf0eZk2KGR? NHXVTFdFVVOR NGTlbY9qdmS3Y3XzJGwyS0GPwrDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?= NHzF[o4zOzV|MEe2PS=>
MFE296 MmrESpVv[3Srb36gRZN{[Xl? NWDjUW9GPTByIH7N NFuwcGE2KGR? M37kSWROW09? M3HKeYlv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF MXGyN|U{ODd4OR?=
HASMCs MYrGeY5kfGmxbjDBd5NigQ>? NUDZboFmOC13MECgcm0> MmnqOkBl M3yzbYRwf25vcnXneYxifGW|IITo[UBmgHC{ZYPzbY9vKG:oIFjBSGMh[W6mIHPlcIwhfmmjYnnsbZR6 M3zQWVI{PTF6NE[3
U373 Ml\kSpVv[3Srb36gRZN{[Xl? NWDxbI91OC5{NT:wMlUwOSEQvF2= NIfHVJAzPCCq MUPpcoNz\WG|ZYOgVHIuSiCneIDy[ZN{cW:wIHPvMZRz\WG2bXXueEB4cXSqIEXBfoFlSw>? NYnkVZF1OjN2N{SxO|E>
ARN8  Mn;LSpVv[3Srb36gRZN{[Xl? MVywMlA2NTJizszN MmrNNlQhcA>? NVnYNoNLemWmdXPld{B1cGViaX7keYN1cW:wIH;mJJA2Oy2mZYDlcoRmdnRiZ3Xu[ZMh[nliToX0cIlvNTN? NWjXUYFMOjN2N{C1OFA>
MCF7 NF3RS|NHfW6ldHnvckBCe3OjeR?= MV:wMlA2NTJizszN NVznNYRMOjRiaB?= MVny[YR2[2W|IITo[UBqdmS3Y4Tpc44hd2ZicEWzMYRmeGWwZHXueEBo\W6nczDifUBPfXSuaX6tNy=> NWrXepNZOjN2N{C1OFA>
H1299  MY\GeY5kfGmxbjDBd5NigQ>? NVq1NXNtOC5|M,MAl|HDqML3TR?= MX:yOE81QCCq MVTEUXNQ NITMZ4RqdmO{ZXHz[ZMhTS2lYXTo[ZJqdiCycn;0[YlvKGyndnXsd{Bld3OnIHTldIVv\GWwdHz5 M4jIZlI{PDZzOUe1
H1299 MXvGeY5kfGmxbjDBd5NigQ>? NFXIRmoxNjVizszN MmDYOFghcA>? MoPDSG1UVw>? MX3pcohq[mm2czD0bIUhdWmpcnH0c5J6KHCxdHXueIlidCClb33ibY5mKHerdHigd4ltcWKrbnnu NGHTVnozOzR4MUm3OS=>
H1299 MWHGeY5kfGmxbjDBd5NigQ>? MkL2NE42KM7:TR?= NWHMNoUyPDhiaB?= MnHMSG1UVw>? NVTUUGU4cW6qaXLpeJMhfGinIHnueoF{cX[nbnXzd:KhKGOxbXLpcoUhf2m2aDDzbYxq[mmwaX6= NFjzT5czOzR4MUm3OS=>
MG-63  NVvMR|dmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzpNE4{KM7:TR?= NIX1b2gyOi17NjDo M{K0eWROW09? NXfrVZhJcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJINwNXS{ZXH0cYVvfCC5aYToJI1mfG[xcn3pci=> MlPrNlM1PTF6MUe=
LM8 NHnWdGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGS3[2kxNjNizszN MV[xNk06PiCq MkfZSG1UVw>? NGnpPVhqdmirYnn0d{B1cGViY3XscEBoem:5dHigZ48ufHKnYYTt[Y51KHerdHigcYV1\m:{bXnu M{fBRVI{PDVzOEG3
K562 MYLGeY5kfGmxbjDBd5NigQ>? NF35SmcxNjVizszN Mnn2NlQhcA>? NFTNNZJmfGijbn;s NGfZfpll\WO{ZXHz[ZMhfGinIHXufplu\SCjY4Tpeol1gSCxZjDISGFEeyClbz30doVifG2nboSge4l1cCCldYLjeY1qdg>? NVrzV3dOOjN2M{C5OVc>
HEL MkewSpVv[3Srb36gRZN{[Xl? NFvSTVYxNjVizszN MlXuNlQhcA>? MWTleIhidm:u NUnYcVd4\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5? NF3QSZgzOzR|MEm1Oy=>
HL60 M3roeWFxd3C2b4Ppd{BCe3OjeR?= MUexJO69VQ>? M{LGblI1KGh? MoLTbY5lfWOnc9MgZ4VtdCCmZXH0bC=> M{D5SFI{PDByNUG5
KG1 Ml7wRZBweHSxc3nzJGF{e2G7 MXuxJO69VQ>? NGDWNpozPCCq NWrVOlBscW6mdXPld:Kh[2WubDDk[YF1cA>? M1fPSFI{PDByNUG5
Kazumi M2XKfGFxd3C2b4Ppd{BCe3OjeR?= NETyeGIyKM7:TR?= M{L1fVI1KGh? Mn;kbY5lfWOnc9MgZ4VtdCCmZXH0bC=> Mnf2NlM1ODB3MUm=
K562 NXzSUYt{SXCxcITvd4l{KEG|c3H5 M{XMOVEh|ryP NX7k[Y03OjRiaB?= MYLpcoR2[2W|wrDj[YxtKGSnYYTo MmrWNlM1ODB3MUm=
THP1 NGTrSHVCeG:ydH;zbZMhSXO|YYm= MUCxJO69VQ>? MonaNlQhcA>? MYPpcoR2[2W|wrDj[YxtKGSnYYTo NVX4e3Y5OjN2MEC1NVk>
SH-SY5Y MWXGeY5kfGmxbjDBd5NigQ>? NVT0clVwOjVyIH7N NIi1R2YyPiCq NUKyXW45[2ijbnfld{BmgHC{ZYPzbY9vKHCjdITldo4hd2ZiZ3Xu[ZMhcW64b3z2[YQhcW5iY3jvcIV{fGW{b3ygd5lvfGinc3nzMEB2eHSja3WgZY5lKGWoZnz1fC=> NHTNVmIzOzN{NkSyNi=>
HEK293 NITiXI1HfW6ldHnvckBCe3OjeR?= NX;uNHRIOSEEtV5CpC=> M{O3N|E5KGkEoB?= NWP4OlFvcW6qaXLpeJMhfGinIHvhcIlzcW5vNz3t[YRq[XSnZDDy[YNzfWm2bXXueEBw\iC|eX7wbIltcW5vMTDh[4dz\WejdHXzJIlvfG9iYXfndoV{d22ncx?= M2ntTVI{Ojh2OES4
HTK MmPOSpVv[3Srb36gRZN{[Xl? MVS0NFAhdk1? NIjFb5Q4OiCq NELT[o1qdmirYnn0d{BVT0ZvzsNihLNKdmS3Y3XkJG16d2[rYoLvZoxie3RiRHnm[oVz\W62aXH0bY9v M4rC[VI{Ojh2MECy
HTK NWjacFBNTnWwY4Tpc44hSXO|YYm= NFrnbpAyODBvOECwcm0> MoiwO|IhcA>? M2TQcIJtd2OtczDUS2Yu|rMkgKPJcoR2[2WmIGLPV{BidmRiSELPNuKhSWOldX31cIF1cW:w NVX3XlhWOjN{OESwNFI>
Caco-2  NELZXHBHfW6ldHnvckBCe3OjeR?= MlKxNUDDvU4EoB?= MWOyOEBp Mof0[IVkemWjc3XzJHNGWlRicILveIVqdiCneIDy[ZN{cW:w M37jZVI{OTl3MEew
HeLa MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\5N|ExOCCwTR?= NEPCco4zPCCq NWTKSIc3TE2VTx?= MU\JR|UxKG:oIEGwNI5O M4HY[|I{OTZ3N{S4
HeLa M{PzWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUS0NEBvVQ>? MmLROFghcA>? MVTEUXNQ MknMTWM2OCCxZjC0NI5O M3PyZVI{OTZ3N{S4
HeLa MlroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjUNlAhdk1? NFn1c3I4OiCq NULOeYVwTE2VTx?= M{Oy[mlEPTBib3[gNlBvVQ>? NGXSNm8zOzF4NUe0PC=>
HeLa MlqzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XWflExNzNyL{WwJI5O NYKwU3c5PzJiaB?= NFfZTHZFVVOR NFLsUIJqdmO{ZXHz[ZMhfGinIH71cYJmeiCxZjDNUXAhMM7WzrjtLUBtd3O|IHPlcIx{KGSxc3Wg[IVx\W6mZX70cJk> MoPxNlMyPjV5NEi=
MDA-MB-231 NXHPOWZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUOyOU01ODBibl2= MoPFOFghcA>? NFrFZo1KSzVyIH;mJFI3Oy5{bl5CpC=> MVGyN|A2PTF7OB?=
MCF-7  MmTMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LxR|I2NTRyMDDuUS=> NE\JSXU1QCCq NELYSFlKSzVyIH;mJFIzOC52bl2= MVqyN|A2PTF7OB?=
ECC-1  MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjVRmgyODBibl2= NITaUogzPCCq MoW5[ZRp[W6xbB?= MX3pcoNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[gZZBweHSxdHnjJI52[2ynaTD0c{A{PSV? NW\vS49nOjNyMki4NFM>
HEC-1A MojTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1P0dFExOCCwTR?= NEjvXZQzPCCq NEjZWGJmfGijbn;s NIDTN41qdmO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh[XCxcITveIlkKG63Y3zlbUB1dyB|OTW= MWqyN|AzQDhyMx?=
NHAC-kn NIflWW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzINVAwOTByL{WwNEBvVQ>? MXmxNkBp M2fWN2ROW09? NGDJVmRKSzVyIH;mJFUxOG6P MnjYNlMxOTd6N{G=
A549 MlLXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLJNlUxKG6P NH\k[Zo3NTd{IHi= NF\BcIlFVVOR M3mwWINifXOnczDhJIdz\WG2ZYKgbY5pcWKrdH;yfUBm\m[nY4SgZ49u[mmwZTD3bZRpKFS[VDDvdkBmemyxdHnubYI> NWfEfVNNOjJ7OUS3PFA>
MG-63 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXWN|AxKG6P M1POUlEzKGh? M1PER2ROW09? MWLpcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gPFYm M4HtflIzPzl7M{O4
MG-63 NXW1UXV6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLON|AxKG6P MmewNlQhcA>? NGTXWVJFVVOR MmLLbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIITvJFY4LQ>? M4jDfFIzPzl7M{O4
MG-63 M3:wWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\6WXZPOzByIH7N M{\Xe|Q5KGh? M3vpTmROW09? NFmzOZBqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPTZn NX7oRlZsOjJ5OUmzN|g>
HL60  MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYi0d|Y6OTVyLUO1NEBvVQ>? NX:ySG1JOjRiaB?= NF\2NItqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O NF3VeZozOjd3M{ezPS=>
U937 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWSxOVAuOzVyIH7N NV;xTVFLOjRiaB?= MmLObY5kemWjc3XzJINmdGxiYYDvdJRwe2m|wrD3bIVvKGOxbnPlcpRz[XSrb37zJIhq\2incjD0bIFvKDJ3MDDuUS=> NXvOd|U3OjJ5NUO3N|k>
SCC-6 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYGyNFAuOzJyMDDuUS=> MXKxNk8zPC92ODDo NXfpWGR3TE2VTx?= NGDCNnRqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBw\iCVQ1OtOkBk\WyuczDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= M1mwNVIzPTV{M{Kx
U87  NWfIe3UxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkL5NVAxNTNyMDDu[y=> NHzLSHEzPCCq MUTpcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gO|ImKCCjdDCyNFBv\w>? NYPLWWVFOjJ{N{C4OFk>
K562 NGXvSHFHfW6ldHnvckBCe3OjeR?= NHHaXG0yKM7:TR?= NGT3bI0yOiCq MXfEUXNQ NEPXdG5mdmijbnPld{B1cGViZYjwdoV{e2mxbjDv[kBTXU6[MzDpcoR2[2WmIHL5JFUu[XqjLVPkVi=> NEm5WWQzOjF5OUG5PC=>
Reh MXTGeY5kfGmxbjDBd5NigQ>? MlX6NE4{NzFizszN MVyxNkBp M13KNmROW09? M4HWOoVvcGGwY3XzJJRp\SCneIDy[ZN{cW:wIH;mJHJWVlh|IHnu[JVk\WRiYomgOU1igmFvQ3TS MoHtNlIyPzlzOUi=

... Click to View More Cell Line Experimental Data

In vivo試験 Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]
臨床試験
特集

プロトコル (参考用のみ)

キナーゼアッセイ:

[1]

In vitro HDAC activity Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.

細胞アッセイ:

[1]

細胞株 MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
濃度 Dissolved in absolute ethanol, final concentrations ~10 μM
反応時間 96 hours
実験の流れ

Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.

動物実験:

[1]

動物モデル Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
製剤 Dissolved in DMSO
投薬量 ~5 mg/kg/day
投与方法 Injection s.c.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDogMonkeyBaboon
Weight (kg)0.020.151.80.40.0810312
Body Surface Area (m2)0.0070.0250.150.050.020.50.240.6
Km factor361285201220
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

参考

化学情報

Download Trichostatin A (TSA) SDF
分子量 302.4
化学式

C17H22N2O3

CAS No. 58880-19-6
保管 2年-20℃
6月-80℃in solvent
別名 N/A
溶解度 (25°C) * In vitro DMSO 23 mg/mL (76.05 mM)
<1 mg/mL (<1 mM)
エタノール <1 mg/mL (<1 mM)
In vivo 1% DMSO+30% polyethylene glycol+1% Tween 80, pH 4 6 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
化学名 (R,2E,4E)-6-(4-(dimethylamino)benzoyl)-N-hydroxy-4-methylhepta-2,4-dienamide

文献中の引用 (20)

技術サポート&よくある質問(FAQ)

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最近チェックしたアイテム

Tags: Trichostatin A (TSA)を買う | Trichostatin A (TSA)供給者 | Trichostatin A (TSA)を購入する | Trichostatin A (TSA)費用 | Trichostatin A (TSA)生産者 | オーダーTrichostatin A (TSA) | Trichostatin A (TSA)代理店
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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