Trichostatin A (TSA) 化学構造
分子量: 302.4

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Quality Control & MSDS

製品説明

  • Compare HDAC Inhibitors
    HDAC製品生物活性の比較
  • 研究分野
  • Combination Therapy
    併用療法
  • Trichostatin A (TSA)のメカニズム

製品の説明

生物活性

製品説明 Trichostatin A (TSA) は、HDAC阻害剤で、IC50 が ~1.8 nM。
ターゲット

HDAC

IC50

~1.8 nM [1]

In vitro試験 Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
HEK 293 MXLGeY5kfGmxbjDBd5NigQ>? NX73ZVVuOC55zszN MXGyOIg> MXfleIhidm:u Mn7j[Y5p[W6lZYOgSW5iSyCjY3X0fYxifGmxbjDhcoQhcW6lcnXhd4V{KEWQYVOgZYJ2dmSjbnPlJIlvKHSqZTD0c5RidCClZXzsJIx6e2G2ZTDhcoQh[XRidHjlJINmdGxic4Xy[oFk\Q>? NGm5NZczPTd6N{C3PS=>
TE13 MWrGeY5kfGmxbjDBd5NigQ>? M1O0OlAvO87:TR?= MYqyOIg> NYLVTpRNfXBvcnXneYxifGW|IGLBV3NHPUFibHX2[Yw> MXuyOVU4QTZ4NR?=
TE13 NEjyOIpCeG:ydH;zbZMhSXO|YYm= NUXZd|lHOC5|zszN NHrjVmEzPGh? NIDYNYFqdmirYnn0d{B1cGViY3XscEBxem:uaX\ldoF1cW:w NV\YeYR5OjV3N{m2OlU>
MEFs M{n2dmZ2dmO2aX;uJGF{e2G7 MX21{txO MorDNVZp NEC4fXRqdmO{ZXHz[ZMhfGinIFXQSWMh[XS2YXPocYVvfCxiVHnyJIRmdGm4ZYL5JIFv\CC2aHWg[YZncWOrZX7jfUBw\iCyZXTld5RidCCob4LtZZRqd25? NUmwfW9uOjV2OEK2N|Q>
SW480  MmPVSpVv[3Srb36gRZN{[Xl? NEnEd2gxNjIQvF2= NIO2eIY1QGh? MVzEUXNQ NHvUOWNz\X[ncoPld{BGVVR? NVTofnhKOjV2M{S5PVc>
PC3  MkLPSpVv[3Srb36gRZN{[Xl? Mk\tNE4y|ryP NVjITGQ6PDiq MoThSG1UVw>? MWLy[ZZmenOnczDFUXQ> MVOyOVQ{PDl7Nx?=
SW480  MVvGeY5kfGmxbjDBd5NigQ>? MUSwMlHPxE1? NF6xW3c1QGh? NF7YNolFVVOR M{HM[IF1fGWwdXH0[ZMhcW64YYPpc44h[W6mIH3p[5JifGmxbh?= MlzaNlU1OzR7OUe=
PC3  MojCSpVv[3Srb36gRZN{[Xl? NWjOUot1OC5zzszN MWq0PIg> NHfqPXlFVVOR NEW4TFJifHSnboXheIV{KGmwdnHzbY9vKGGwZDDtbYdz[XSrb36= M{nJelI2PDN2OUm3
SW480  M4nqcWZ2dmO2aX;uJGF{e2G7 MX2wMlHPxE1? Ml7EOFhp M3nXXGROW09? NHS3NJFqdmS3Y3XzJIlv[3KnYYPlJI9nKEiGQVOxJIFv\CCKRFHDNkBwdiCVbIXnJIdmdmW|IIDyc41wfGW{ MYWyOVQ{PDl7Nx?=
PC3  M3vtUmZ2dmO2aX;uJGF{e2G7 M3ToTFAvOc7:TR?= Moj5OFhp NV22R2kzTE2VTx?= M4jJfolv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ? M2nGdlI2PDN2OUm3
A431 NV\FO3BuSXCxcITvd4l{KEG|c3H5 MUSyM|ExNzVyL{GwNI5O M4Pt[lQ5cA>? M3TJSWROW09? NXnYNlM{cW6qaXLpeJMhfGinIHPlcIwh\3Kxd4To M{XPU|I2OzdzME[5
A431 MWjGeY5kfGmxbjDBd5NigQ>? MonPOVBvVQ>? MoHHNk83NzF{L{K0bC=> M3zKRmROW09? MYThZ5RqfmG2ZYOgdFIyKGGwZDDpcohq[mm2czDBWGY{KGW6cILld5Nqd25? M3yzN|I2OzdzME[5
MDA-MB-231 NWTxUnJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnryNE03ODCwTR?= NUTFVmRWOjSq M1TjSGROW09? NHX3SXdKSzVyIH;mJFExOG6P MnfDNlUyQTJ5MkG=
MCF7 NVPnfGR5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPVUWkxNTZyMH7N NYWwW5Z1OjSq M1rQVGROW09? NWDofFRnUUN3MDDv[kA4PW6P M3SzRlI2OTl{N{Kx
SKOV-3 NXzGZ5E{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUSxMVEx|ryP Mn7tNlRp NVvzUFdtTE2VTx?= NUTR[WRlUUN3MDDv[kA2NjcQvF2= NFrMbIszPTF4OUS5NS=>
A549 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVmxMVEx|ryP M1HSXlI1cA>? MWPEUXNQ NVTqUHVHUUN3MDDv[kA{NjMQvF2= M3HLUVI2OTZ7NEmx
SKOV-3 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWf3VYZtOC5zLUJOwG0> NITMO2w1QGh? MUfEUXNQ Ml\vTWM2OCCxZjCwMlfPxE1? MVmyOVE3QTR7MR?=
A549 NIK5fYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1O4OlAvOS1zzszN NVnRS41vPDiq M1mxWGROW09? M1OwfWlEPTBib3[gNE4zQM7:TR?= NHz2d|YzPTF4OUS5NS=>
SKOV-3 M2rpcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGGxPIYxNjBzLUCuPe69VQ>? NI\1Onc4Omh? NGPXZlVFVVOR NEW2ZWJKSzVyIH;mJFAvOzMQvF2= M4HBXFI2OTZ7NEmx
A549 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXOwMlAyNTBwOd88US=> MUG3Nog> M1jRe2ROW09? M2G3TmlEPTBib3[gNE4xPs7:TR?= MonoNlUyPjl2OUG=
HeLa MofLSpVv[3Srb36gRZN{[Xl? M2HTTFI2OG6P MWKxOog> MXLEUXNQ NUTvWWlYcW6lcnXhd4V{KEO\UEHBNUBuWk6DIHX4dJJme3Orb39CpC=> Ml;iNlUyOTZ4OEi=
CNE2 M4\pc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\ENVAxNTZyMH7N MViyOE81QC95Mni= NIT0N2tqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBqdiCjIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MX:yOFk3QTlyMR?=
PC3 NGjYZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWOxNFAuOTByMH7N MWSyOIg> M{L1cGlEPTBib3[gN|Axdk1? M37aflI1QDV2NkW4
LNCaP M4\NNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXW2dWNxOTByLUGwNFBvVQ>? NVjXUVRoOjSq MVnJR|UxKG:oIEOwNI5O MV2yOFg2PDZ3OB?=
HeLa  NWS5[mlwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\WfWxFOs7:TR?= NXfJdYVjPDiq M1HHZYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDhZo92fCB{NTW= NFfrcVczPDh2NkGzOS=>
HMEC-1  NWTySnVWTnWwY4Tpc44hSXO|YYm= M4K5UFMxOG6P M3H5XlI1cA>? MnnxbY5kemWjc3XzJIV5eHKnc4Ppc44hd2cEoG\FS2ZTO8LibWLORS=> MUWyOFcyODZ|MR?=
HeLa M165N2Z2dmO2aX;uJGF{e2G7 MnPuNY1O MWmwMlVp NFrGU4Ni[m:uaYPo[ZMhfGinIITveIFtKEiGQVOgZYN1cX[rdIm= MYCyOFcxPzR5NB?=
ACP02 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWqyOVAwOzVyL{WwNI5O MV2yOIg> M4HUdIRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDv[kBieHC{b4jpcYF1\Wy7IEewxsAmKGG2IEK1NOKhdk1? NXzUfHd{OjR4Nki1OFc>
ACP03 M{PiVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWqyOVAwOzVyL{WwNI5O MXWyOIg> M3zSfoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDv[kBieHC{b4jpcYF1\Wy7IEewxsAmKGG2IEK1NOKhdk1? NU\NR3dkOjR4Nki1OFc>
U87 GBM MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXixNFAwOzByL{WwNEBvVQ>? NXzYZ4hsPzJiaB?= NUO1fpZ{OTByJTDleIhidm:u MkTwdoVlfWOnczDt[YFvKGOnbHygcpVu[mW{IHL5JFMyNCB3NDygZY5lKDV6wrCl NIrYTFgzPDR4NEi0NS=>
U87 GBM M4f0TmZ2dmO2aX;uJGF{e2G7 NYjaO2FwOTByL{WwNEBPdQ>? Ml\VOFghcA>? M1LGT|ExOCViZYToZY5wdA>? M3\SOGlv\HWlZYOgV4Vv\XOlZX7j[U1NcWunIFHseIVz[XSrb37zJIlvKE63Y3zlZZIhVW:{cHjvcI9ogQ>? MlLhNlQ1PjR6NEG=
RPE NILJToRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV2wMlIwOC52L{CuPE8yKM7:TR?= NHXG[XIzPC92OD:3NkBp M4OzRWROW09? NXTafopPcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44h[nliY3XscEBkgWOuZTDhdpJme3R? NUnoc3h7OjR2NU[2NFI>
HT29  MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{C1XFE5OCCwTR?= MYm3NkBp NX:5RnZtUUN3MDDv[kAyQDBibl2= M3u0XFI1OzZ6Mk[1
hMSCs M2njSGZ2dmO2aX;uJGF{e2G7 MWK2MlI2KG6P M2m2c|I1KGh? MmjJSG1UVw>? M{TjU5N1[WKrbHn6[ZMhUGm|dH;u[UBC[2W2eXzheIlwdiCjbnSgeIhmKEW6cILld5Nqd25ib3[gVIx2emmyb4TlcpQhT2WwZYO= MV2yOFMyOjN3Nh?=
Huh7  MYHGeY5kfGmxbjDBd5NigQ>? NV7oZo5HOC5zL{CuOU8yNjBizszN M1T0fFI1cA>? M4XEOJN2eHC{ZYPz[ZMhYVSKRFOyJIdmdmViZYjwdoV{e2mxbh?= NU\adnJnOjR{Nkm2O|I>
SKOV3 NIrmU4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4[zPFAvODVvMjFOwG0> M2rp[|ExNzJ2L{S4JIg> NV\CR5pHTE2VTx?= M125N41m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ Mn;4NlQzOjN6MEG=
A2780 MmO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXGN2gxNjB3LUKg{txO NXTnXItwOTBxMkSvOFghcA>? Mn7mSG1UVw>? NFjlV3hu\WSrYYTld{Boem:5dHigZZJz\XO2IHnuJIEh[2:wY3XueJJifGmxbj2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MlXnNlQzOjN6MEG=
SRA01/04  NEfkfINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTEepMxNjJizszN NXjjNoU4PDhiaB?= NIn6OVFFVVOR Mkf3d5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM> NHLnT2ozPDF3N{i3PC=>
HLEB3 Moj3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vzWFAvOiEQvF2= MXq0PEBp NFHTXYlFVVOR M3zpOZN2eHC{ZYPz[ZMh[2WubDDwdo9tcW[ncnH0bY9vKHSqcn;1[4ghe3WycILld5NqdmdidHjlJHBKO0txQXv0MEBxOziPQWDLJIFv\CCHUluxM|Ihe2mpbnHsbY5oKHCjdHj3ZZl{ MXiyOFE2Pzh5OB?=
SRA01/04  NFvtdoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXqyS2VIOC52L{CuPEDPxE1? NXvOZ|hyPDhiaB?= MUjEUXNQ NYXKdVRLcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NVXaVmhMOjRzNUe4O|g>
HLEB3 Mny5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEixXmQxNjRxMD64JO69VQ>? NUnuO3A{PDhiaB?= NUjBeYl[TE2VTx?= MnnZbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? NUXsWpZlOjRzNUe4O|g>
HCT116 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\uVI0xNjJizszN MWOxNkBp NEjWbnhmdmijbnPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JJJi\GmjdHnvci=> NVv3e2JTOjRzMkKyN|E>
CA46 M1;sfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFX6em0{NzZxMUKvNlQwPDhibl2= MmLqOFghcA>? MlTNbY5pcWKrdIOgeIhmKGOnbHygdJJwdGmoZYLheIlwdiCjdDCyOEBidmRiNEigcm3DqA>? NWS4[3VmOjRyNkS5OVE>
PMNs M4fITWZ2dmO2aX;uJGF{e2G7 NGm5d5Y{OCCwTR?= NIS4fHg1QCCq MXHpcohq[mm2czDl[oZmem:leYTvd4l{NCCKRFHDJIFkfGm4aYT5JIFv\CCUYXOgZYN1cX[rdIm= MkjCNlM6QDh4MUe=
H1299 Ml3qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfGSpl2OcLizszNxsA> NFj2cnEzPC92OD:3NkBp NVH0V5F1\XSqYX7vcC=> Mnm2bY5kemWjc3XzJINmdGxiZHXheIgh[W[2ZYKgNlRp NGjmNWgzOzlzNk[wPS=>
A549 NXrEO|ZRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHkNE4zPS9yLkWvNUDPxE1? MUGyOE81QC95MjDo MULleIhidm:u MYnpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX6gZ49v[2WwdILheIlwdiCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz MnfXNlM5Pjd7OUG=
H1299 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvTR5FtOC5{NT:wMlUwOSEQvF2= MV[yOE81QC95MjDo MoO4[ZRp[W6xbB?= MXHpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX6gZ49v[2WwdILheIlwdiCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz MlmxNlM5Pjd7OUG=
A549 NVy2OW17SXCxcITvd4l{KEG|c3H5 M2H5TlAvPS9zIN88US=> NHq3do81QCCq MVnleIhidm:u MW\pcoR2[2W|IHPlcIwh[XCxcITvd4l{ MnXYNlM5Pjd7OUG=
H1299 M{\mSWFxd3C2b4Ppd{BCe3OjeR?= NWPXWm1WOC53L{Gg{txO MmLtOFghcA>? MkDN[ZRp[W6xbB?= NIfiPIRqdmS3Y3XzJINmdGxiYYDvdJRwe2m| MkD1NlM5Pjd7OUG=
SUM149PT MoDxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHRdYczNzdwNT:xNEDPxE1? MoT2OFghcA>? MnrISG1UVw>? NGDzXIlqdmS3Y3XzJINmdGxiZHXheIgh[XRiMjFOwG0hcW6|ZX7zbZRqfmWueR?= M1HKclI{Pzl{NkO4
SUM190PT M3KzT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGraTFY2OC9zMECvNlUxKG6P M2j6c|Q5KGh? M2rXW2ROW09? NXriXZE1cW6mdXPld{Bk\WyuIHTlZZRpKGG2IEWwJI5OKHOnboPpeIl3\Wy7 MlniNlM4QTJ4M{i=
HCT1 MlXIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHq0NmkxNjJxMT6wM|UvOCEQvF2= M2jvclEzNzJ2L{O2M|Q5KGh? M{PKNolv\HWlZYOgZ4VtdCCmZXH0bEBqdiClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NFq0WFIzOzd5MECwNC=>
Lovo NYPlN|VNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYqwMlIwOS5yL{WuNEDPxE1? MnvRNVIwOjRxM{[vOFghcA>? NWrpVFZTcW6mdXPld{Bk\WyuIHTlZZRpKGmwIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M330WFI{PzdyMECw
AGS MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPVfWkxNjBzNT2xJO69VQ>? Ml\oO|IhcA>? MkHVbY5lfWOnczDj[YxtKGSnYYToJINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= M2LWd|I{PzR3MEK0
Huh7 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TJRVEh|ryP Mlr5NlQhcA>? Ml7NSG1UVw>? NWTZfohbemWmdXPld{Bi[m:3dDCyNEUhfmmjYnnsbZR6yqB? M{SzZ|I{PjR|OUOz
ECC1 NUXUXVQ6TnWwY4Tpc44hSXO|YYm= MXy1NFAhdk1? NUTVcWhrPSCm NUnpPGFCTE2VTx?= M2DEeolv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF NGq1[G4zOzV|MEe2PS=>
HEC1A M4H4UGZ2dmO2aX;uJGF{e2G7 M2[5N|UxOCCwTR?= MlSzOUBl NVi1NmhrTE2VTx?= MUPpcoR2[2W|IFyxR2FOyqCneIDy[ZN{cW:wIHPvMZRz\WG2bXXueEB4cXSqIEWtRZpiSw>? NHHw[WIzOzV|MEe2PS=>
EN1 MXvGeY5kfGmxbjDBd5NigQ>? MkLBOVAxKG6P M3fwTVUh\A>? NHvpNGJFVVOR Ml3MbY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN? M3HDN|I{PTNyN{[5
MFE296 NHXwb|RHfW6ldHnvckBCe3OjeR?= NInuNmI2ODBibl2= MoHMOUBl MYfEUXNQ MYjpcoR2[2W|IFyxR2FOyqCneIDy[ZN{cW:wIHPvMZRz\WG2bXXueEB4cXSqIEWtRZpiSw>? MnHVNlM2OzB5Nkm=
HASMCs MXjGeY5kfGmxbjDBd5NigQ>? NHjKNW4xNTVyMDDuUS=> NYXpdpl2PiCm MUnkc5dvNXKnZ4XsZZRmeyC2aHWg[ZhxemW|c3nvckBw\iCKQVTDJIFv\CClZXzsJJZq[WKrbHn0fS=> MYmyN|UyQDR4Nx?=
U373 NULiSINDTnWwY4Tpc44hSXO|YYm= M2rYb|AvOjVxMD61M|Eh|ryP NXPlTGRSOjRiaB?= NGTFSYxqdmO{ZXHz[ZMhWFJvQjDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFVCgmGmQx?= MmDQNlM1PzRzN{G=
ARN8  MmrRSpVv[3Srb36gRZN{[Xl? NYPodI0xOC5yNT2yJO69VQ>? MYqyOEBp NI\WXYVz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gdFU{NWSncHXu[IVvfCCpZX7ld{BjgSCQdYTsbY4uOw>? MYmyN|Q4ODV2MB?=
MCF7 NXOw[lMzTnWwY4Tpc44hSXO|YYm= M3S3dVAvODVvMjFOwG0> NUjCUoFIOjRiaB?= NIjjbHNz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gdFU{NWSncHXu[IVvfCCpZX7ld{BjgSCQdYTsbY4uOw>? MknzNlM1PzB3NEC=
H1299  NEHuS5pHfW6ldHnvckBCe3OjeR?= MXewMlM{6oDVMdMgxtVO M1\Od|I1NzR6IHi= NF\1O4NFVVOR MXzpcoNz\WG|ZYOgSU1k[WSqZYLpckBxem:2ZXnuJIxmfmWuczDkc5NmKGSncHXu[IVvfGy7 MnHCNlM1PjF7N{W=
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LM8 MmX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHZ[5AxNjNizszN M3rKUVEzNTl4IHi= NGjBSGVFVVOR NWfqZ3FHcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJINwNXS{ZXH0cYVvfCC5aYToJI1mfG[xcn3pci=> MWqyN|Q2OThzNx?=
K562 NXTrSGxbTnWwY4Tpc44hSXO|YYm= MkTLNE42KM7:TR?= MVKyOEBp M{\GdoV1cGGwb3y= NEDZU|Bl\WO{ZXHz[ZMhfGinIHXufplu\SCjY4Tpeol1gSCxZjDISGFEeyClbz30doVifG2nboSge4l1cCCldYLjeY1qdg>? Mn7sNlM1OzB7NUe=
HEL MX;GeY5kfGmxbjDBd5NigQ>? Mlv5NE42KM7:TR?= NYfGbpQ{OjRiaB?= NYS0RYw3\XSqYX7vcC=> Mofn[IVkemWjc3XzJJRp\SCnbor5cYUh[WO2aY\peJkhd2ZiSFTBR5Mh[29vdILlZZRu\W62IIfpeIgh[3W{Y4XtbY4> NVP0Z41vOjN2M{C5OVc>
HL60 MkPWRZBweHSxc3nzJGF{e2G7 M4P4bVEh|ryP NGTsRpAzPCCq NWD1c|B[cW6mdXPld:Kh[2WubDDk[YF1cA>? NHX6cGkzOzRyMEWxPS=>
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Kazumi NXzubJdPSXCxcITvd4l{KEG|c3H5 MYOxJO69VQ>? NVjD[JR5OjRiaB?= NEPKXGhqdmS3Y3XzxsBk\WyuIHTlZZRp M2LzUFI{PDByNUG5
K562 NEHoW5VCeG:ydH;zbZMhSXO|YYm= MVSxJO69VQ>? MXmyOEBp MlS5bY5lfWOnc9MgZ4VtdCCmZXH0bC=> M2XmSVI{PDByNUG5
THP1 NGDp[mpCeG:ydH;zbZMhSXO|YYm= Mn\tNUDPxE1? NYnHZ29COjRiaB?= NUG1c5M4cW6mdXPld:Kh[2WubDDk[YF1cA>? MXeyN|QxODVzOR?=
SH-SY5Y Mo\YSpVv[3Srb36gRZN{[Xl? NVH1TVRpOjVyIH7N NWTqbZY5OTZiaB?= MXHjbIFv\2W|IHX4dJJme3Orb36gdIF1fGW{bjDv[kBo\W6nczDpcpZwdH[nZDDpckBkcG:uZYP0[ZJwdCC|eX70bIV{cXNuIIXweIFs\SCjbnSg[YZndHW6 NEjjUXkzOzN{NkSyNi=>
HEK293 M2DybWZ2dmO2aX;uJGF{e2G7 NGnjcGkyKML3TdMg M1rkb|E5KGkEoB?= MVnpcohq[mm2czD0bIUhc2GuaYLpck04NW2nZHnheIVlKHKnY4L1bZRu\W62IH;mJJN6dnCqaXzpck0yKGGpZ4Ll[4F1\XNiaX70c{Bi\2e{ZYPvcYV{ NWK3V5NLOjN{OES4OFg>
HTK M{WyVGZ2dmO2aX;uJGF{e2G7 NFzlcGs1ODBibl2= MYS3NkBp M1Pn[4lvcGmkaYTzJHRITi4QsvMAl2lv\HWlZXSgUZlw\mmkcn;icIF{fCCGaX\m[ZJmdnSrYYTpc44> M1ux[FI{Ojh2MECy
HTK NYTt[YNNTnWwY4Tpc44hSXO|YYm= NHjnSGgyODBvOECwcm0> MWO3NkBp NXX0SnZ5[myxY3vzJHRITi4QsvMAl2lv\HWlZXSgVm9UKGGwZDDINm8zyqCDY3P1cZVt[XSrb36= MUCyN|I5PDByMh?=
Caco-2  MVXGeY5kfGmxbjDBd5NigQ>? NFvqNXEyKML3TdMg MXGyOEBp NWnI[|hI\GWlcnXhd4V{KFOHUmSgdJJwfGWrbjDlfJBz\XO|aX;u NHvQ[nUzOzF7NUC3NC=>
HeLa NFrNdXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofDNVAxKG6P MlXvNlQhcA>? NGjTe2VFVVOR Mn\ZTWM2OCCxZjCxNFBvVQ>? MoTxNlMyPjV5NEi=
HeLa NX\MblZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfy[GVTPDBibl2= NXjaOJZuPDhiaB?= MXLEUXNQ MYfJR|UxKG:oIESwcm0> MX:yN|E3PTd2OB?=
HeLa M3WycGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfLNlAhdk1? NVSwbJF3PzJiaB?= NXvvZlFUTE2VTx?= M{HrNGlEPTBib3[gNlBvVQ>? M1;IfFI{OTZ3N{S4
HeLa NWnBeJpxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnObFAyOC9|MD:1NEBvVQ>? MYK3NkBp M2\ve2ROW09? NYDyb5ZQcW6lcnXhd4V{KHSqZTDueY1j\XJib3[gUW1RKCkQlN8ocUkhdG:|czDj[YxteyCmb4PlJIRmeGWwZHXueIx6 MlnjNlMyPjV5NEi=
MDA-MB-231 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXyyOU01ODBibl2= NHXrcVI1QCCq MUfJR|UxKG:oIEK2N{4zdk4EoB?= MnLzNlMxPTVzOUi=
MCF-7  NX7lRVY2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXuXHUzPS12MECgcm0> MoXZOFghcA>? MlL2TWM2OCCxZjCyNlAvPG6P MYCyN|A2PTF7OB?=
ECC-1  M4\RSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvHZ4QxOTByIH7N MUmyOEBp M4exboV1cGGwb3y= M1\0fYlv[3KnYYPld{B1cGVicILvdI9zfGmxbjDv[kBieG:ydH;0bYMhdnWlbHXpJJRwKDN3JR?= M{XOUVI{ODJ6OECz
HEC-1A NFjXUJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TZOFExOCCwTR?= NXzYOXlSOjRiaB?= MWnleIhidm:u MWjpcoNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[gZZBweHSxdHnjJI52[2ynaTD0c{A{QSV? NUP5RYJkOjNyMki4NFM>
NHAC-kn MnuyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUOxNE8yODBxNUCwJI5O MW[xNkBp MULEUXNQ NYTC[VFxUUN3MDDv[kA2ODCwTR?= MXWyN|AyPzh5MR?=
A549 M4T0Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3RV3ZQOjVyIH7N M4PtSlYuPzJiaB?= NXnGb4MzTE2VTx?= MUjjZZV{\XNiYTDndoVifGW{IHnubIljcXSxcomg[YZn\WO2IHPvcYJqdmVid3n0bEBVYFRib4Kg[ZJtd3Srbnni Ml\UNlI6QTR5OEC=
MG-63 M{S3fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXWzNFAhdk1? NVXpdZRtOTJiaB?= NIjhcYFFVVOR NUXWRZk5cW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJJRwKDh4JR?= M2\Zb|IzPzl7M{O4
MG-63 M4XtNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3S2XVMxOCCwTR?= MYSyOEBp M3\MbWROW09? MW\pcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gOlcm M1fOTVIzPzl7M{O4
MG-63 M4q3Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzMZmk{ODBibl2= MmPxOFghcA>? NYrSSoF5TE2VTx?= NHjnU5NqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPTZn MoPjNlI4QTl|M{i=
HL60  NV7PcZR1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDWTFJrOTVyLUO1NEBvVQ>? NXruc4lIOjRiaB?= NFrrclVqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O MWKyNlc2Ozd|OR?=
U937 NG\FenRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13CW|E2OC1|NUCgcm0> NEDMd|EzPCCq NGLP[pNqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O MYCyNlc2Ozd|OR?=
SCC-6 Mo\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX[yNFAuOzJyMDDuUS=> NVL4bpE{OTJxMkSvOFghcA>? MlH6SG1UVw>? NIPucWdqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBw\iCVQ1OtOkBk\WyuczDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NHjQU5czOjV3MkOyNS=>
U87  MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPHNVAxNTNyMDDu[y=> M2CxWFI1KGh? MkHDbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIITvJFczLSBiYYSgNlAxdmd? MWSyNlI4ODh2OR?=
K562 NFPoZ2dHfW6ldHnvckBCe3OjeR?= NGGwe4UyKM7:TR?= MWmxNkBp MlPYSG1UVw>? MX\lcohidmOnczD0bIUh\XiycnXzd4lwdiCxZjDSWW5ZOyCrbnT1Z4VlKGK7IEWtZZpiNUOmUh?= NV3G[XlSOjJzN{mxPVg>
Reh NEXXdnlHfW6ldHnvckBCe3OjeR?= M3HJc|AvOy9zIN88US=> M1PsVlEzKGh? NGP3bpNFVVOR Mm\0[Y5p[W6lZYOgeIhmKGW6cILld5Nqd25ib3[gVnVPYDNiaX7keYNm\CCkeTC1MYF7[S2FZGK= NVPNUI1tOjJzN{mxPVg>

... Click to View More Cell Line Experimental Data

In vivo試験 Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]
臨床試験
特集

プロトコル (参考用のみ)

キナーゼアッセイ:

[1]

In vitro HDAC activity Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.

細胞アッセイ:

[1]

細胞株 MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
濃度 Dissolved in absolute ethanol, final concentrations ~10 μM
反応時間 96 hours
実験の流れ

Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.

動物実験:

[1]

動物モデル Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
製剤 Dissolved in DMSO
投薬量 ~5 mg/kg/day
投与方法 Injection s.c.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDogMonkeyBaboon
Weight (kg)0.020.151.80.40.0810312
Body Surface Area (m2)0.0070.0250.150.050.020.50.240.6
Km factor361285201220
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

参考

化学情報

Download Trichostatin A (TSA) SDF
分子量 302.4
化学式

C17H22N2O3

CAS No. 58880-19-6
保管 2年-20℃
6月-80℃in solvent
別名 N/A
溶解度 (25°C) * In vitro DMSO 23 mg/mL (76.05 mM)
<1 mg/mL (<1 mM)
エタノール <1 mg/mL (<1 mM)
In vivo 1% DMSO+30% polyethylene glycol+1% Tween 80, pH 4 6 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
化学名 (R,2E,4E)-6-(4-(dimethylamino)benzoyl)-N-hydroxy-4-methylhepta-2,4-dienamide

カスタマーフィードバック (4)


Click to enlarge
Rating
Source Epigenetics, 2012, 7(10):1161-72. Trichostatin A (TSA) purchased from Selleck
Method Immunofluorescence staining
Cell Lines H1299 cells
Concentrations 0.5 uM
Incubation Time 48 h
Results To identify whether these G2/M fractions of cells were arrested at G2 phase or undergoing a mitotic arrest, we analyzed 48 h treated cells for the expression of TSA and Saha, p-histone H3 Ser 10 and p-MPM-2Ser /Thr. As indicated by decreased nuclear expression of p-histone H3 Ser 10, quantitative analysis of immunofluorescence (IF) data showed a significant decrease in mitotic index (% of cells in mitotic phase) by combinatorial treatments. This was further corroborated by decreased expression of MPM-2 reactive structured components of the mitotic apparatus that are required for both onset and completion of M-phase.

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Rating
Source Biochem Biophys Res Commun, 2014, 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines HCT116 p53 null cells
Concentrations 1 uM
Incubation Time 24 h
Results TSA causes ER stress and increase the expression of ATF4, ATF3, CHOP.

Click to enlarge
Rating
Source Biochem Biophys Res Commun, 2014, 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines HCT116 p53 null cells
Concentrations 2 uM
Incubation Time 24 h
Results Knockdown of ATF3 suppressed the induction of DR5 protein and knockdown of ATF4 suppressed the induction of ATF3, CHOP and DR5 proteins by these HDACIs(TSA, M344, MS-275).

Click to enlarge
Rating
Source 2010, Dr. Zhang of Tianjin Medical University. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines MDA-MB-231 cells
Concentrations 0-20 μM
Incubation Time
Results Reduction of Histone H3 acetylation in MDA-MB-231 cells treated with Trichostatin A was observed.

文献中の引用 (20)

技術サポート&よくある質問(FAQ)

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Tags: Trichostatin A (TSA)を買う | Trichostatin A (TSA)供給者 | Trichostatin A (TSA)を購入する | Trichostatin A (TSA)費用 | Trichostatin A (TSA)生産者 | オーダーTrichostatin A (TSA) | Trichostatin A (TSA)代理店
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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