Trichostatin A (TSA) 化学構造
分子量: 302.4

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Quality Control & MSDS

製品説明

  • Compare HDAC Inhibitors
    HDAC製品生物活性の比較
  • 研究分野
  • Combination Therapy
    併用療法
  • Trichostatin A (TSA)のメカニズム

製品の説明

生物活性

製品説明 Trichostatin A (TSA) は、HDAC阻害剤で、IC50 が ~1.8 nM。
ターゲット

HDAC

IC50

~1.8 nM [1]

In vitro試験 Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
HEK 293 MkLHSpVv[3Srb36gRZN{[Xl? NIXp[3MxNjgQvF2= MnzrNlRp MXTleIhidm:u MYLlcohidmOnczDFUoFEKGGlZYT5cIF1cW:wIHHu[EBqdmO{ZXHz[ZMhTU6jQzDhZpVv\GGwY3WgbY4hfGinIITveIFtKGOnbHygcJl{[XSnIHHu[EBifCC2aHWgZ4VtdCC|dYLmZYNm MXSyOVc5PzB5OR?=
TE13 NFHXTJJHfW6ldHnvckBCe3OjeR?= MUmwMlPPxE1? NH24bmczPGh? M3rxXZVxNXKnZ4XsZZRmeyCUQWPTSlVCKGyndnXs M1jpVVI2PTd7Nk[1
TE13 MYTBdI9xfG:|aYOgRZN{[Xl? NXfZO2s4OC5|zszN NXrESHBROjSq M3zJW4lvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25? MmjQNlU2Pzl4NkW=
MEFs M3fJNGZ2dmO2aX;uJGF{e2G7 MVi1{txO MlLHNVZp MlHzbY5kemWjc3XzJJRp\SCHUFXDJIF1fGGlaH3lcpQtKFSrcjDk[YxqfmW{eTDhcoQhfGinIHXm[olkcWWwY4mgc4YheGWmZYP0ZYwh\m:{bXH0bY9v M{XIZ|I2PDh{NkO0
SW480  Ml;4SpVv[3Srb36gRZN{[Xl? MX2wMlHPxE1? NF3ZeZA1QGh? MYPEUXNQ MYHy[ZZmenOnczDFUXQ> MmPiNlU1OzR7OUe=
PC3  MWfGeY5kfGmxbjDBd5NigQ>? M2GzV|AvOc7:TR?= MX[0PIg> NG\CdXVFVVOR NWrsVIJ2emW4ZYLz[ZMhTU2W NX\kOYpKOjV2M{S5PVc>
SW480  NHnibZVHfW6ldHnvckBCe3OjeR?= MVGwMlHPxE1? MV[0PIg> NV32eIFoTE2VTx?= M3TZ[oF1fGWwdXH0[ZMhcW64YYPpc44h[W6mIH3p[5JifGmxbh?= NIrLZWgzPTR|NEm5Oy=>
PC3  NHe5TJJHfW6ldHnvckBCe3OjeR?= MojKNE4y|ryP M2PkelQ5cA>? MVnEUXNQ MXfheJRmdnWjdHXzJIlvfmG|aX;uJIFv\CCvaXfyZZRqd25? M4PseVI2PDN2OUm3
SW480  M4T4c2Z2dmO2aX;uJGF{e2G7 Mn7RNE4y|ryP M1PDV|Q5cA>? NHPMRoFFVVOR MlzTbY5lfWOnczDpcoNz\WG|ZTDv[kBJTEGFMTDhcoQhUESDQ{Kgc44hW2y3ZzDn[Y5meyCycn;tc5Rmeg>? Ml7kNlU1OzR7OUe=
PC3  MVHGeY5kfGmxbjDBd5NigQ>? M2nMXFAvOc7:TR?= NVrXNZB7PDiq MYTEUXNQ MWXpcoR2[2W|IHnuZ5Jm[XOnIH;mJGhFSUNzIHHu[EBJTEGFMjDvckBUdHWpIHflcoV{KHC{b33veIVz M3naZVI2PDN2OUm3
A431 MmjrRZBweHSxc3nzJGF{e2G7 NV7JdG9WOi9zMD:1NE8yODCwTR?= M1npfVQ5cA>? NUKxR|hDTE2VTx?= MV7pcohq[mm2czD0bIUh[2WubDDndo94fGh? NHXydogzPTN5MUC2PS=>
A431 NVPyS3B1TnWwY4Tpc44hSXO|YYm= NHftUo42OG6P NIjTWmgzNzZxMUKvNlRp MUPEUXNQ NVPKPHhs[WO2aY\heIV{KHB{MTDhcoQhcW6qaXLpeJMhSVSIMzDlfJBz\XO|aX;u NF\GN4YzPTN5MUC2PS=>
MDA-MB-231 Moq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXGPGwxNTZyMH7N M3r5UFI1cA>? MUXEUXNQ MlnETWM2OCCxZjCxNFBvVQ>? M{XJU|I2OTl{N{Kx
MCF7 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\BWplTOC14MEDuUS=> NFfuT2IzPGh? MlL4SG1UVw>? MkjlTWM2OCCxZjC3OY5O NFj6flgzPTF7MkeyNS=>
SKOV-3 MlPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzxNU0yOM7:TR?= NYi5c4JCOjSq NVzKfHlWTE2VTx?= M{\mVGlEPTBib3[gOU43|ryP MnzwNlUyPjl2OUG=
A549 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXy0WpJYOS1zMN88US=> NEHRbmYzPGh? NHfuVG5FVVOR NY\GdnRCUUN3MDDv[kA{NjMQvF2= MknINlUyPjl2OUG=
SKOV-3 M3y2T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrzWYgxNjFvMd88US=> M3\JcFQ5cA>? MX\EUXNQ Mn:wTWM2OCCxZjCwMlfPxE1? NWrvVpFbOjVzNkm0PVE>
A549 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLY[FZzOC5zLUJOwG0> MXS0PIg> MnXKSG1UVw>? NXT4NWhqUUN3MDDv[kAxNjJ6zszN NUXYO4UyOjVzNkm0PVE>
SKOV-3 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnwNE4xOS1yLkpOwG0> MonyO|Jp NX22V4hlTE2VTx?= MlPOTWM2OCCxZjCwMlMz|ryP MoHHNlUyPjl2OUG=
A549 MnTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUKwMlAyNTBwOd88US=> NH;hZWo4Omh? MWLEUXNQ M3nnRmlEPTBib3[gNE4xPs7:TR?= MYOyOVE3QTR7MR?=
HeLa MWnGeY5kfGmxbjDBd5NigQ>? NGn4c3UzPTCwTR?= MV[xOog> NVzvNoRmTE2VTx?= Mne4bY5kemWjc3XzJGN[WDGDMTDtVm5CKGW6cILld5Nqd28EoB?= NYrlZXdZOjVzMU[2PFg>
CNE2 Mm\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfzUVJbOTByLU[wNI5O NYD4epVtOjRxNEivO|Jp MXnpcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MX2yOFk3QTlyMR?=
PC3 NXnRdZFLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDSNVAxNTFyMEDuUS=> NWe4T2JJOjSq NXLJfFU6UUN3MDDv[kA{ODCwTR?= MYmyOFg2PDZ3OB?=
LNCaP M2XZOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXxepIyODBvMUCwNI5O Mn\YNlRp M4nWRmlEPTBib3[gN|Axdk1? MWKyOFg2PDZ3OB?=
HeLa  NF7lWldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVyy{txO NXnEXlJ4PDiq NX[5e5Jn\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIFjd3W2IEK1KS=> M1\LXVI1QDR4MUO1
HMEC-1  Ml;aSpVv[3Srb36gRZN{[Xl? MUCzNFBvVQ>? NV\xV5l2OjSq NH;mRmtqdmO{ZXHz[ZMh\XiycnXzd4lwdiCxZtMgWmVITlJ|wrDtVm5C NH3kTmwzPDdzME[zNS=>
HeLa M2fP[GZ2dmO2aX;uJGF{e2G7 MmrlNY1O M2K2NFAvPWh? MoPRZYJwdGm|aHXzJJRp\SC2b4ThcEBJTEGFIHHjeIl3cXS7 MViyOFcxPzR5NB?=
ACP02 MkfpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TNbVI2OC9|NUCvOVAxdk1? MXuyOIg> NV2zboZP\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JI9nKGGycILvfIlu[XSnbImgO|DDqCViYYSgNlUxyqCwTR?= NXvO[mc2OjR4Nki1OFc>
ACP03 NY\FV2dzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nlNFI2OC9|NUCvOVAxdk1? MW[yOIg> MmPa[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>? Mk\VNlQ3Pjh3NEe=
U87 GBM MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHMOI8yODBxM{CwM|UxOCCwTR?= M1j2fFczKGh? MUmxNFAmKGW2aHHuc4w> MYTy[YR2[2W|IH3lZY4h[2WubDDueY1j\XJiYomgN|EtKDV2LDDhcoQhPTkEoDW= NYHDUpJHOjR2NkS4OFE>
U87 GBM MmDVSpVv[3Srb36gRZN{[Xl? NWXn[oE1OTByL{WwNEBPdQ>? NXPTZ3F1PDhiaB?= MUSxNFAmKGW2aHHuc4w> MXzJcoR2[2W|IGPlcoV{[2WwY3WtUIls\SCDbITldoF1cW:wczDpckBPfWOuZXHyJG1wenCqb3zv[5k> NYPQbYhLOjR2NkS4OFE>
RPE NVXnemtxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYKwMlIwOC52L{CuPE8yKM7:TR?= MXqyOE81QC95MjDo M3m1emROW09? MXTpcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDifUBk\WyuIHP5Z4xmKGG{cnXzeC=> Ml;nNlQ1PTZ4MEK=
HT29  MmnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\P[IVtOThyIH7N MmLJO|IhcA>? M3zvRWlEPTBib3[gNVgxKG6P NHzLNGQzPDN4OEK2OS=>
hMSCs NXHi[m9PTnWwY4Tpc44hSXO|YYm= MkTWOk4zPSCwTR?= M3rifFI1KGh? M1r5dWROW09? NV;KOolDe3SjYnnsbZpmeyCKaYP0c45mKEGlZYT5cIF1cW:wIHHu[EB1cGViRYjwdoV{e2mxbjDv[kBRdHW{aYDveIVvfCCJZX7ldy=> MnzYNlQ{OTJ|NU[=
Huh7  NEDjXVRHfW6ldHnvckBCe3OjeR?= M2fXRlAvOS9yLkWvNU4xKM7:TR?= MkfhNlRp M2HDOZN2eHC{ZYPz[ZMhYVSKRFOyJIdmdmViZYjwdoV{e2mxbh?= M2TEU|I1OjZ7Nkey
SKOV3 NEPHSo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU[0PY5sOC5yNT2yJO69VQ>? MUmxNE8zPC92ODDo NVGwTXI6TE2VTx?= MWTt[YRq[XSnczDndo94fGhiYYLy[ZN1KGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MX:yOFIzOzhyMR?=
A2780 Ml:5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvRWWQxNjB3LUKg{txO NXzXPWU3OTBxMkSvOFghcA>? MYjEUXNQ NIjRcFRu\WSrYYTld{Boem:5dHigZZJz\XO2IHnuJIEh[2:wY3XueJJifGmxbj2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MkjkNlQzOjN6MEG=
SRA01/04  NXfpTFk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PRPVAvOiEQvF2= MYW0PEBp NF7IcJNFVVOR MmLwd5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM> M32y[FI1OTV5OEe4
HLEB3 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnT6NE4zKM7:TR?= MnLWOFghcA>? MmDpSG1UVw>? MXLzeZBxemW|c3XzJINmdGxicILvcIln\XKjdHnvckB1cHKxdXfoJJN2eHC{ZYPzbY5oKHSqZTDQTVNMN0GtdDygdFM5VUGSSzDhcoQhTVKNMT:yJJNq\26jbHnu[{Bx[XSqd3H5dy=> MUiyOFE2Pzh5OB?=
SRA01/04  NWrkNYZ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVmwMlQwOC56IN88US=> M{DxV|Q5KGh? NHy3e5lFVVOR NVjNS2RXcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NXfXNXBpOjRzNUe4O|g>
HLEB3 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrINE41NzBwODFOwG0> M2fkUlQ5KGh? NVH1[GR4TE2VTx?= M4X4cYlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? NGnHUHQzPDF3N{i3PC=>
HCT116 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHCNG4xNjJizszN NE\KbWwyOiCq MX3lcohidmOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IILh[IlifGmxbh?= Mnn1NlQyOjJ{M{G=
CA46 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW[zM|YwOTJxMkSvOFghdk1? M4fERVQ5KGh? MnrFbY5pcWKrdIOgeIhmKGOnbHygdJJwdGmoZYLheIlwdiCjdDCyOEBidmRiNEigcm3DqA>? MV2yOFA3PDl3MR?=
PMNs MXLGeY5kfGmxbjDBd5NigQ>? NYDi[JNuOzBibl2= NEjnVVA1QCCq MWrpcohq[mm2czDl[oZmem:leYTvd4l{NCCKRFHDJIFkfGm4aYT5JIFv\CCUYXOgZYN1cX[rdIm= MljBNlM6QDh4MUe=
H1299 M2fKVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoT2NeKh|ryPwrC= M2r1NVI1NzR6L{eyJIg> MkjY[ZRp[W6xbB?= MYTpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBi\nSncjCyOIg> NXXvfmxPOjN7MU[2NFk>
A549 NEO5TFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGKxNXAxNjJ3L{CuOU8yKM7:TR?= MYKyOE81QC95MjDo NYnZT3BN\XSqYX7vcC=> NXTDNoRbcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJIlvKGOxbnPlcpRz[XSrb36gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MljGNlM5Pjd7OUG=
H1299 MlrzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDNWHp5OC5{NT:wMlUwOSEQvF2= NI\VenMzPC92OD:3NkBp MkDC[ZRp[W6xbB?= NIDxSmpqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY4h[2:wY3XueJJifGmxbjDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ MkfMNlM5Pjd7OUG=
A549 MVPBdI9xfG:|aYOgRZN{[Xl? M3S0[|AvPS9zIN88US=> NVXsRXhWPDhiaB?= NV3ZRpZm\XSqYX7vcC=> M4Lwc4lv\HWlZYOgZ4VtdCCjcH;weI9{cXN? NW\Kc5ZtOjN6Nke5PVE>
H1299 M4PxVWFxd3C2b4Ppd{BCe3OjeR?= M1nnXVAvPS9zIN88US=> NV3L[YkyPDhiaB?= NXz6UFhk\XSqYX7vcC=> NX\H[pJFcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MX:yN|g3Pzl7MR?=
SUM149PT NIm0SmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjsNk84NjVxMUCg{txO NXjsd4ZpPDhiaB?= M3izbGROW09? NIfOd4FqdmS3Y3XzJINmdGxiZHXheIgh[XRiMjFOwG0hcW6|ZX7zbZRqfmWueR?= M3G5W|I{Pzl{NkO4
SUM190PT NFXnfWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVe1NE8yODBxMkWwJI5O MUe0PEBp MkLwSG1UVw>? NHfW[|NqdmS3Y3XzJINmdGxiZHXheIgh[XRiNUCgcm0he2Wwc3n0bZZmdHl? MYeyN|c6OjZ|OB?=
HCT1 Mm\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnLR4MxNjJxMT6wM|UvOCEQvF2= NELCXZcyOi9{ND:zOk81QCCq MWXpcoR2[2W|IHPlcIwh\GWjdHigbY4h[2:wY3XueJJifGmxbj2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MWeyN|c4ODByMB?=
Lovo MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nkS|AvOi9zLkCvOU4xKM7:TR?= M4raO|EzNzJ2L{O2M|Q5KGh? M3PlVolv\HWlZYOgZ4VtdCCmZXH0bEBqdiClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MnzMNlM4PzByMEC=
AGS M2H3NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrGOHFvOC5yMUWtNUDPxE1? M2nnUlczKGh? NXSyR5MxcW6mdXPld{Bk\WyuIHTlZZRpKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> M1TGd|I{PzR3MEK0
Huh7 M3HKWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHsNUDPxE1? MYqyOEBp MYHEUXNQ MY\y[YR2[2W|IHHic5V1KDJyJTD2bYFjcWyrdIpCpC=> MV2yN|Y1Ozl|Mx?=
ECC1 M3vpZ2Z2dmO2aX;uJGF{e2G7 MVW1NFAhdk1? MVm1JIQ> NGj1RolFVVOR NXzMOndocW6mdXPld{BNOUODTdMg[ZhxemW|c3nvckBkdy22cnXheI1mdnRid3n0bEA2NUG8YVO= NWro[YFVOjN3M{C3Olk>
HEC1A NYHlXldkTnWwY4Tpc44hSXO|YYm= NV7IUndSPTByIH7N M{\jR|Uh\A>? NYLqcWVYTE2VTx?= M1fQ[4lv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF NELQ[GczOzV|MEe2PS=>
EN1 NEDnRVlHfW6ldHnvckBCe3OjeR?= NW\oPHVQPTByIH7N MWe1JIQ> NVzCUZJFTE2VTx?= M{XrOolv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF M1HE[VI{PTNyN{[5
MFE296 M{XhfWZ2dmO2aX;uJGF{e2G7 MlLzOVAxKG6P MkPjOUBl NXrj[mhbTE2VTx?= NE\SfZZqdmS3Y3XzJGwyS0GPwrDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?= NFfRWVczOzV|MEe2PS=>
HASMCs MmHZSpVv[3Srb36gRZN{[Xl? NET5SoIxNTVyMDDuUS=> M2LjdlYh\A>? MkTX[I94di2{ZXf1cIF1\XNidHjlJIV5eHKnc4Ppc44hd2ZiSFHER{BidmRiY3XscEB3cWGkaXzpeJk> MX2yN|UyQDR4Nx?=
U373 MkfXSpVv[3Srb36gRZN{[Xl? MknTNE4zPS9yLkWvNUDPxE1? MlLuNlQhcA>? NF\SWZdqdmO{ZXHz[ZMhWFJvQjDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFVCgmGmQx?= NGnz[2szOzR5NEG3NS=>
ARN8  NHnycnJHfW6ldHnvckBCe3OjeR?= MYmwMlA2NTJizszN NG[0b3MzPCCq NVG3SmxtemWmdXPld{B1cGViaX7keYN1cW:wIH;mJJA2Oy2mZYDlcoRmdnRiZ3Xu[ZMh[nliToX0cIlvNTN? NGTSd5EzOzR5MEW0NC=>
MCF7 Mlm4SpVv[3Srb36gRZN{[Xl? NW\FTXo1OC5yNT2yJO69VQ>? MlzTNlQhcA>? M{P5[ZJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDwOVMu\GWyZX7k[Y51KGenbnXzJIJ6KE63dHzpck0{ NGXiTVIzOzR5MEW0NC=>
H1299  NInXZmNHfW6ldHnvckBCe3OjeR?= NV23[3dHOC5|M,MAl|HDqML3TR?= M4DxZlI1NzR6IHi= NEDsdpZFVVOR NWD5R5NmcW6lcnXhd4V{KEVvY3HkbIVzcW5icILveIVqdiCuZY\lcJMh\G:|ZTDk[ZBmdmSnboTsfS=> NFzVbnozOzR4MUm3OS=>
H1299 NInCOoFHfW6ldHnvckBCe3OjeR?= NVrwW4JqOC53IN88US=> MXe0PEBp NI\NRmlFVVOR NWHURmQxcW6qaXLpeJMhfGinIH3p[5JifG:{eTDwc5RmdnSrYXygZ49u[mmwZTD3bZRpKHOrbHnibY5qdg>? NUPCZ2F2OjN2NkG5O|U>
H1299 MnXlSpVv[3Srb36gRZN{[Xl? NV35VINPOC53IN88US=> M{mwSlQ5KGh? NUC3fGRzTE2VTx?= NIO4S4FqdmirYnn0d{B1cGViaX72ZZNqfmWwZYPzxsAh[2:vYnnu[UB4cXSqIIPpcIljcW6rbh?= MkTYNlM1PjF7N{W=
MG-63  MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\4eJExNjNizszN NUPVTm97OTJvOU[gbC=> MnPLSG1UVw>? MnnKbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHPvMZRz\WG2bXXueEB4cXSqIH3leIZwem2rbh?= MX[yN|Q2OThzNx?=
LM8 NGXaXHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYCwMlMh|ryP M2PjVFEzNTl4IHi= MVjEUXNQ MUjpcohq[mm2czD0bIUh[2WubDDndo94fGhiY3:teJJm[XSvZX70JJdqfGhibXX0[o9zdWmw MofmNlM1PTF6MUe=
K562 MkfPSpVv[3Srb36gRZN{[Xl? NHXnXYIxNjVizszN M3S2cFI1KGh? NIq0UlNmfGijbn;s NXHCV2lu\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5? MWeyN|Q{ODl3Nx?=
HEL NXXqNHNlTnWwY4Tpc44hSXO|YYm= MUKwMlUh|ryP NFLaZ5AzPCCq M4m0SIV1cGGwb3y= NYTzeIpq\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5? NH3aVFgzOzR|MEm1Oy=>
HL60 MV\BdI9xfG:|aYOgRZN{[Xl? M{Kx[|Eh|ryP MUWyOEBp MYXpcoR2[2W|wrDj[YxtKGSnYYTo NWfSVngxOjN2MEC1NVk>
KG1 M3rremFxd3C2b4Ppd{BCe3OjeR?= Moj6NUDPxE1? M1m0RVI1KGh? NX7R[4RpcW6mdXPld:Kh[2WubDDk[YF1cA>? M1r2bVI{PDByNUG5
Kazumi Mn7JRZBweHSxc3nzJGF{e2G7 MlfxNUDPxE1? NIrydVQzPCCq MYnpcoR2[2W|wrDj[YxtKGSnYYTo NFS2PJYzOzRyMEWxPS=>
K562 NULLfpd1SXCxcITvd4l{KEG|c3H5 NYD1XXR7OSEQvF2= NETRflMzPCCq MVrpcoR2[2W|wrDj[YxtKGSnYYTo MoHzNlM1ODB3MUm=
THP1 Ml:yRZBweHSxc3nzJGF{e2G7 M2HFblEh|ryP NVj2ZZFbOjRiaB?= M4\sfolv\HWlZYRCpINmdGxiZHXheIg> NFnTeGMzOzRyMEWxPS=>
SH-SY5Y MoH6SpVv[3Srb36gRZN{[Xl? MWiyOVAhdk1? MkX2NVYhcA>? NGHEclFkcGGwZ3XzJIV5eHKnc4Ppc44heGG2dHXyckBw\iCpZX7ld{Bqdn[xbI\l[EBqdiClaH;s[ZN1\XKxbDDzfY51cGW|aYOsJJVxfGGtZTDhcoQh\W[obIX4 NES4ZVYzOzN{NkSyNi=>
HEK293 NWXLd|RxTnWwY4Tpc44hSXO|YYm= MX:xJOK2VcLi MWWxPEBpyqB? MlX3bY5pcWKrdIOgeIhmKGujbHnybY4uPy2vZXTpZZRm\CC{ZXPyeYl1dWWwdDDv[kB{gW6yaHnsbY4uOSCjZ3fy[YdifGW|IHnueI8h[WepcnXzc41mew>? NIXmU5czOzJ6NEi0PC=>
HTK NHPPb4dHfW6ldHnvckBCe3OjeR?= NEeyXG01ODBibl2= MUO3NkBp NHe0e21qdmirYnn0d{BVT0ZvzsNihLNKdmS3Y3XkJG16d2[rYoLvZoxie3RiRHnm[oVz\W62aXH0bY9v NWLFd4VsOjN{OESwNFI>
HTK MVXGeY5kfGmxbjDBd5NigQ>? NWi3OFB1OTByLUiwNI5O NEPP[W84OiCq NF\2XW5jdG:la4OgWGdHNc7{4pETTY5lfWOnZDDSU3Mh[W6mIFiyU|LDqEGlY4XteYxifGmxbh?= MW[yN|I5PDByMh?=
Caco-2  NIPScFFHfW6ldHnvckBCe3OjeR?= MVuxJOK2VcLi NVnhRlB2OjRiaB?= M1HPUIRm[3KnYYPld{BUTVKWIIDyc5RmcW5iZYjwdoV{e2mxbh?= NGKycWwzOzF7NUC3NC=>
HeLa MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn[wNVAxKG6P M2[xelI1KGh? NXXBNYdFTE2VTx?= MV7JR|UxKG:oIEGwNI5O NHXpe24zOzF4NUe0PC=>
HeLa MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrNb4ozPDBibl2= NHj2dJY1QCCq M2fZd2ROW09? M3fqTGlEPTBib3[gOFBvVQ>? Ml;rNlMyPjV5NEi=
HeLa Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX2yNEBvVQ>? MmXqO|IhcA>? M{fOU2ROW09? M1ny[mlEPTBib3[gNlBvVQ>? NWrGdHB[OjNzNkW3OFg>
HeLa MnfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTzTIE5OTBxM{CvOVAhdk1? NIHRSI44OiCq MUDEUXNQ NH7XTGNqdmO{ZXHz[ZMhfGinIH71cYJmeiCxZjDNUXAhMM7WzrjtLUBtd3O|IHPlcIx{KGSxc3Wg[IVx\W6mZX70cJk> NFXu[pQzOzF4NUe0PC=>
MDA-MB-231 NWHNUZA4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVH4R|RKOjVvNECwJI5O NVvSUGVuPDhiaB?= M3zJdmlEPTBib3[gNlY{NjKwTdMg MX:yN|A2PTF7OB?=
MCF-7  MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY[yOU01ODBibl2= NVH3[VNlPDhiaB?= MlO3TWM2OCCxZjCyNlAvPG6P MWeyN|A2PTF7OB?=
ECC-1  NVu3WnNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnMNVAxKG6P NYS0bppHOjRiaB?= M4\BR4V1cGGwb3y= MV7pcoNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[gZZBweHSxdHnjJI52[2ynaTD0c{A{PSV? NYnYXHM5OjNyMki4NFM>
HEC-1A MnfRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDoNVAxKG6P M3HBRlI1KGh? M3nsZoV1cGGwb3y= NWDNPXRxcW6lcnXhd4V{KHSqZTDwdo9xd3K2aX;uJI9nKGGyb4D0c5Rq[yCwdXPs[YkhfG9iM{ml Mnj2NlMxOjh6MEO=
NHAC-kn NYjmcGxTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4m4dVExNzFyMD:1NFAhdk1? NFHBSlYyOiCq MVLEUXNQ MoPLTWM2OCCxZjC1NFBvVQ>? MXmyN|AyPzh5MR?=
A549 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVi5SVBJOjVyIH7N M2jNSlYuPzJiaB?= MonzSG1UVw>? MXvjZZV{\XNiYTDndoVifGW{IHnubIljcXSxcomg[YZn\WO2IHPvcYJqdmVid3n0bEBVYFRib4Kg[ZJtd3Srbnni NF60VoczOjl7NEe4NC=>
MG-63 NEKy[IRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIO1dYY{ODBibl2= NH;sUnIyOiCq NXvse2xzTE2VTx?= NYfhemFHcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJJRwKDh4JR?= MV2yNlc6QTN|OB?=
MG-63 Mk\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17hTlMxOCCwTR?= NWTRcZJXOjRiaB?= M3HteWROW09? NGXGbHZqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPjdn MkPiNlI4QTl|M{i=
MG-63 M{HjcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrlN|AxKG6P MUS0PEBp M1v0eWROW09? NXHnOINMcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJJRwKDV4JR?= NELTRlEzOjd7OUOzPC=>
HL60  MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;pZm8yPTBvM{WwJI5O M2XOVVI1KGh? MVzpcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXQEoIfo[Y4h[2:wY3XueJJifGmxboOgbIlocGW{IIToZY4hOjVyIH7N NUPHRYZiOjJ5NUO3N|k>
U937 M{G0bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXyxOVAuOzVyIH7N NYPaOYZ5OjRiaB?= NIDGfYlqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O M4XvSlIzPzV|N{O5
SCC-6 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXKeVZwOjByLUOyNFAhdk1? MkjzNVIwOjRxNEigbC=> NYTPRWZ1TE2VTx?= MlP6bY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gc4YhW0OFLU[gZ4VtdHNiaX6gZUBld3OnLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ M3\rWVIzPTV{M{Kx
U87  M12xUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEfF[nMyODBvM{CwJI5o NUjMcppKOjRiaB?= NGrqTWVqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPzJnIDDheEAzODCwZx?= MmSyNlIzPzB6NEm=
K562 M1y3PWZ2dmO2aX;uJGF{e2G7 MWqxJO69VQ>? NIHlR48yOiCq M2jW[2ROW09? NXfB[XJM\W6qYX7j[ZMhfGinIHX4dJJme3Orb36gc4YhWlWQWEOgbY5lfWOnZDDifUA2NWG8YT3D[HI> MVSyNlE4QTF7OB?=
Reh NY[yRWJ{TnWwY4Tpc44hSXO|YYm= MkTGNE4{NzFizszN NWLlZVk3OTJiaB?= MVnEUXNQ MWDlcohidmOnczD0bIUh\XiycnXzd4lwdiCxZjDSWW5ZOyCrbnT1Z4VlKGK7IEWtZZpiNUOmUh?= NVTOW2hIOjJzN{mxPVg>

... Click to View More Cell Line Experimental Data

In vivo試験 Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]
臨床試験
特集

プロトコル (参考用のみ)

キナーゼアッセイ:

[1]

In vitro HDAC activity Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.

細胞アッセイ:

[1]

細胞株 MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
濃度 Dissolved in absolute ethanol, final concentrations ~10 μM
反応時間 96 hours
実験の流れ

Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.

動物実験:

[1]

動物モデル Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
製剤 Dissolved in DMSO
投薬量 ~5 mg/kg/day
投与方法 Injection s.c.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDogMonkeyBaboon
Weight (kg)0.020.151.80.40.0810312
Body Surface Area (m2)0.0070.0250.150.050.020.50.240.6
Km factor361285201220
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

参考

化学情報

Download Trichostatin A (TSA) SDF
分子量 302.4
化学式

C17H22N2O3

CAS No. 58880-19-6
保管 2年-20℃
6月-80℃in solvent
別名 N/A
溶解度 (25°C) * In vitro DMSO 23 mg/mL (76.05 mM)
<1 mg/mL (<1 mM)
エタノール <1 mg/mL (<1 mM)
In vivo 1% DMSO+30% polyethylene glycol+1% Tween 80, pH 4 6 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
化学名 (R,2E,4E)-6-(4-(dimethylamino)benzoyl)-N-hydroxy-4-methylhepta-2,4-dienamide

カスタマーフィードバック (4)


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Rating
Source Epigenetics, 2012, 7(10):1161-72. Trichostatin A (TSA) purchased from Selleck
Method Immunofluorescence staining
Cell Lines H1299 cells
Concentrations 0.5 uM
Incubation Time 48 h
Results To identify whether these G2/M fractions of cells were arrested at G2 phase or undergoing a mitotic arrest, we analyzed 48 h treated cells for the expression of TSA and Saha, p-histone H3 Ser 10 and p-MPM-2Ser /Thr. As indicated by decreased nuclear expression of p-histone H3 Ser 10, quantitative analysis of immunofluorescence (IF) data showed a significant decrease in mitotic index (% of cells in mitotic phase) by combinatorial treatments. This was further corroborated by decreased expression of MPM-2 reactive structured components of the mitotic apparatus that are required for both onset and completion of M-phase.

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Rating
Source Biochem Biophys Res Commun, 2014, 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines HCT116 p53 null cells
Concentrations 1 uM
Incubation Time 24 h
Results TSA causes ER stress and increase the expression of ATF4, ATF3, CHOP.

Click to enlarge
Rating
Source Biochem Biophys Res Commun, 2014, 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines HCT116 p53 null cells
Concentrations 2 uM
Incubation Time 24 h
Results Knockdown of ATF3 suppressed the induction of DR5 protein and knockdown of ATF4 suppressed the induction of ATF3, CHOP and DR5 proteins by these HDACIs(TSA, M344, MS-275).

Click to enlarge
Rating
Source 2010, Dr. Zhang of Tianjin Medical University. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines MDA-MB-231 cells
Concentrations 0-20 μM
Incubation Time
Results Reduction of Histone H3 acetylation in MDA-MB-231 cells treated with Trichostatin A was observed.

文献中の引用 (20)

技術サポート&よくある質問(FAQ)

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Tags: Trichostatin A (TSA)を買う | Trichostatin A (TSA)供給者 | Trichostatin A (TSA)を購入する | Trichostatin A (TSA)費用 | Trichostatin A (TSA)生産者 | オーダーTrichostatin A (TSA) | Trichostatin A (TSA)代理店
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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