Trichostatin A (TSA) 化学構造
分子量: 302.4

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Quality Control & MSDS

製品説明

  • Compare HDAC Inhibitors
    HDAC製品生物活性の比較
  • 研究分野
  • Combination Therapy
    併用療法
  • Trichostatin A (TSA)のメカニズム

製品の説明

生物活性

製品説明 Trichostatin A (TSA) は、HDAC阻害剤で、IC50 が ~1.8 nM。
ターゲット

HDAC

IC50

~1.8 nM [1]

In vitro試験 Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
HEK 293 MYnGeY5kfGmxbjDBd5NigQ>? MXqwMlfPxE1? NFP1N3UzPGh? M1fGTIV1cGGwb3y= MYflcohidmOnczDFUoFEKGGlZYT5cIF1cW:wIHHu[EBqdmO{ZXHz[ZMhTU6jQzDhZpVv\GGwY3WgbY4hfGinIITveIFtKGOnbHygcJl{[XSnIHHu[EBifCC2aHWgZ4VtdCC|dYLmZYNm MkWzNlU4QDdyN{m=
TE13 NH7MPHRHfW6ldHnvckBCe3OjeR?= M1zvW|AvO87:TR?= MXSyOIg> NEH1VVd2eC2{ZXf1cIF1\XNiUlHTV2Y2SSCuZY\lcC=> MoO2NlU2Pzl4NkW=
TE13 M3LybWFxd3C2b4Ppd{BCe3OjeR?= NVPoZnZ6OC5|zszN MoDFNlRp MYTpcohq[mm2czD0bIUh[2WubDDwdo9tcW[ncnH0bY9v MYeyOVU4QTZ4NR?=
MEFs NI[yfolHfW6ldHnvckBCe3OjeR?= Moq1Oe69VQ>? MU[xOog> Mn\2bY5kemWjc3XzJJRp\SCHUFXDJIF1fGGlaH3lcpQtKFSrcjDk[YxqfmW{eTDhcoQhfGinIHXm[olkcWWwY4mgc4YheGWmZYP0ZYwh\m:{bXH0bY9v MYKyOVQ5OjZ|NB?=
SW480  Mn3wSpVv[3Srb36gRZN{[Xl? Mn;vNE4y|ryP M1HXdFQ5cA>? NWXqdZdITE2VTx?= NV;zXW4xemW4ZYLz[ZMhTU2W NGjvNngzPTR|NEm5Oy=>
PC3  M3\hWGZ2dmO2aX;uJGF{e2G7 NFfFOJQxNjIQvF2= MkHBOFhp NEf3PHBFVVOR MlLXdoV3\XK|ZYOgSW1V MX:yOVQ{PDl7Nx?=
SW480  MorqSpVv[3Srb36gRZN{[Xl? NH7zO3MxNjIQvF2= MYm0PIg> MVfEUXNQ Mnf2ZZR1\W63YYTld{Bqdn[jc3nvckBidmRibXnndoF1cW:w MX6yOVQ{PDl7Nx?=
PC3  MkDRSpVv[3Srb36gRZN{[Xl? NH7lRZcxNjIQvF2= MlLCOFhp MX3EUXNQ NFLJcI9ifHSnboXheIV{KGmwdnHzbY9vKGGwZDDtbYdz[XSrb36= MoTPNlU1OzR7OUe=
SW480  NWnjdm44TnWwY4Tpc44hSXO|YYm= MUewMlHPxE1? NWrXRZFzPDiq Mk\5SG1UVw>? NUjaOnJGcW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?= Mk\SNlU1OzR7OUe=
PC3  MkHySpVv[3Srb36gRZN{[Xl? NVHJRZZkOC5zzszN NEDLfng1QGh? NFHN[XdFVVOR NHzWOGFqdmS3Y3XzJIlv[3KnYYPlJI9nKEiGQVOxJIFv\CCKRFHDNkBwdiCVbIXnJIdmdmW|IIDyc41wfGW{ M1nvXFI2PDN2OUm3
A431 NV7lRoNTSXCxcITvd4l{KEG|c3H5 MYeyM|ExNzVyL{GwNI5O NVTtc413PDiq MVXEUXNQ NV;jXGk3cW6qaXLpeJMhfGinIHPlcIwh\3Kxd4To NEjJT4YzPTN5MUC2PS=>
A431 MVvGeY5kfGmxbjDBd5NigQ>? MkfzOVBvVQ>? MkXzNk83NzF{L{K0bC=> M13xe2ROW09? NUPTdY0{[WO2aY\heIV{KHB{MTDhcoQhcW6qaXLpeJMhSVSIMzDlfJBz\XO|aX;u MmL5NlU{PzFyNkm=
MDA-MB-231 NWr5ZVNjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\IVXYxNTZyMH7N NGDuVFgzPGh? M3;uUWROW09? NVLTcZh2UUN3MDDv[kAyODCwTR?= M{TKUVI2OTl{N{Kx
MCF7 MnWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnBVYNNOC14MEDuUS=> NFPwdYIzPGh? M{HXOmROW09? MUfJR|UxKG:oIEe1cm0> MnLTNlUyQTJ5MkG=
SKOV-3 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33nZ|EuOTEQvF2= M2raXlI1cA>? NF\RNIRFVVOR NUOzS4hKUUN3MDDv[kA2NjcQvF2= NUC5dVh[OjVzNkm0PVE>
A549 NIryOGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvlVJIyNTFyzszN MljZNlRp MoLFSG1UVw>? M4DVT2lEPTBib3[gN{4z|ryP MlTuNlUyPjl2OUG=
SKOV-3 NF\F[2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7vd2dlOC5zLUJOwG0> NV3sUHJqPDiq MnvpSG1UVw>? NHu1RZBKSzVyIH;mJFAvP87:TR?= M1vW[|I2OTZ7NEmx
A549 NUHLbXpCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;rTlhlOC5zLUJOwG0> NUDlfYhYPDiq NVL4O3dNTE2VTx?= M2S3NWlEPTBib3[gNE4zQM7:TR?= NUexSVhKOjVzNkm0PVE>
SKOV-3 NEXuSmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jCUFAvODFvMD65{txO M2XuW|czcA>? NYG0ZoJXTE2VTx?= M17FZ2lEPTBib3[gNE4{Os7:TR?= MYeyOVE3QTR7MR?=
A549 NUfRNJpyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjFb2NyOC5yMT2wMlnPxE1? MV23Nog> Mn;pSG1UVw>? NXHBVY1lUUN3MDDv[kAxNjB4zszN MXiyOVE3QTR7MR?=
HeLa MmnwSpVv[3Srb36gRZN{[Xl? M37UdFI2OG6P MkLkNVZp MmfkSG1UVw>? M3:1NIlv[3KnYYPld{BEYVBzQUGgcXJPSSCneIDy[ZN{cW:wwrC= MUeyOVEyPjZ6OB?=
CNE2 M1Kydmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljRNVAxNTZyMH7N NUHqNHFbOjRxNEivO|Jp M4Hh[IlvcGmkaYTzJJRp\SCycn;sbYZmemG2aX;uJIlvKGFidHnt[U0h[W6mIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NFW4[oEzPDl4OUmwNS=>
PC3 NEC2e25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGewfZIyODBvMUCwNI5O NFPFeIkzPGh? MXPJR|UxKG:oIEOwNI5O M2XISlI1QDV2NkW4
LNCaP MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\ve|ExOC1zMECwcm0> MnXjNlRp NX76Z5M3UUN3MDDv[kA{ODCwTR?= M1H2S|I1QDV2NkW4
HeLa  NHS5b3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mln3Nu69VQ>? M2HLV|Q5cA>? Mn\X[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHHic5V1KDJ3JR?= NULkXnFwOjR6NE[xN|U>
HMEC-1  MWfGeY5kfGmxbjDBd5NigQ>? MnPNN|Axdk1? NUTR[WYzOjSq NYPrT2xFcW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>? Mnf4NlQ4OTB4M{G=
HeLa MXfGeY5kfGmxbjDBd5NigQ>? NHj3OoEydU1? MVSwMlVp M120TIFjd2yrc3jld{B1cGVidH;0ZYwhUESDQzDhZ5Rqfmm2eR?= MnqzNlQ4ODd2N{S=
ACP02 M{jqNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXsNGJJOjVyL{O1NE82ODCwTR?= MYKyOIg> NXi5W245\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JI9nKGGycILvfIlu[XSnbImgO|DDqCViYYSgNlUxyqCwTR?= NV3HSZlIOjR4Nki1OFc>
ACP03 MkjPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU[yOVAwOzVyL{WwNI5O NGX3[3ozPGh? NIrtUnVl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgc4Yh[XCycn;4bY1ifGWueTC3NOKhLSCjdDCyOVDDqG6P MVuyOFY3QDV2Nx?=
U87 GBM NWTWSWt2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33pblExOC9|MECvOVAxKG6P NYX6W4NWPzJiaB?= MXmxNFAmKGW2aHHuc4w> MXvy[YR2[2W|IH3lZY4h[2WubDDueY1j\XJiYomgN|EtKDV2LDDhcoQhPTkEoDW= MWGyOFQ3PDh2MR?=
U87 GBM NHrXbIRHfW6ldHnvckBCe3OjeR?= NYfwN4pwOTByL{WwNEBPdQ>? NHryWW01QCCq MXixNFAmKGW2aHHuc4w> NXvJVndEUW6mdXPld{BU\W6nc3PlcoNmNUyra3WgRYx1\XKjdHnvcpMhcW5iToXjcIVieiCPb4LwbI9td2e7 Mn64NlQ1PjR6NEG=
RPE MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnXNE4zNzBwND:wMlgwOSEQvF2= MXuyOE81QC95MjDo M36zTGROW09? M{jJeYlvcGmkaYTzJJRp\SCycn;sbYZmemG2aX;uJIJ6KGOnbHygZ5lkdGViYYLy[ZN1 MkLuNlQ1PTZ4MEK=
HT29  NVXOeoZxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWSxPFAhdk1? NY\1blE3PzJiaB?= NEjqZYxKSzVyIH;mJFE5OCCwTR?= MmHsNlQ{Pjh{NkW=
hMSCs M3\LemZ2dmO2aX;uJGF{e2G7 NV3EUodmPi5{NTDuUS=> NHzvTlUzPCCq MVfEUXNQ NX2wWJhHe3SjYnnsbZpmeyCKaYP0c45mKEGlZYT5cIF1cW:wIHHu[EB1cGViRYjwdoV{e2mxbjDv[kBRdHW{aYDveIVvfCCJZX7ldy=> MlO1NlQ{OTJ|NU[=
Huh7  MXjGeY5kfGmxbjDBd5NigQ>? NHr3SGUxNjFxMD61M|EvOCEQvF2= Mnm5NlRp NH7YbW9{fXCycnXzd4V{KFmWSFTDNkBo\W6nIHX4dJJme3Orb36= NF73eGwzPDJ4OU[3Ni=>
SKOV3 M3jCSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3Twe|AvODVvMjFOwG0> NHPQbnUyOC9{ND:0PEBp MlzBSG1UVw>? Mlj1cYVlcWG2ZYOg[5Jwf3SqIHHydoV{fCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NEL2V5IzPDJ{M{iwNS=>
A2780 NITlb2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjFNE4xPS1{IN88US=> M17VdlExNzJ2L{S4JIg> MUnEUXNQ M{\0R41m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NFG0cFQzPDJ{M{iwNS=>
SRA01/04  Ml:wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jOeFAvOiEQvF2= MVS0PEBp MWfEUXNQ NVLnXlJCe3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC|dYDwdoV{e2mwZzD0bIUhWEl|Sz;Bb5QtKHB|OF3BVGsh[W6mIFXST|EwOiC|aXfuZYxqdmdicHH0bJdigXN? Mn[xNlQyPTd6N{i=
HLEB3 M4LYVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fQNFAvOiEQvF2= MorYOFghcA>? MX3EUXNQ MoO5d5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM> Moj2NlQyPTd6N{i=
SRA01/04  MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Cxc|AvPC9yLkig{txO NX;DW|BxPDhiaB?= NVrQRWdtTE2VTx?= M4C2cIlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MYCyOFE2Pzh5OB?=
HLEB3 NWWyWZpkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnG0NE41NzBwODFOwG0> Ml7qOFghcA>? NY\XfpNCTE2VTx?= MUTpcoR2[2W|IHPlcIwh[XCxcITvd4l{ M33oelI1OTV5OEe4
HCT116 NHjzU4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfEPVAxNjJizszN NGHLVo0yOiCq M1zubYVvcGGwY3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgdoFlcWG2aX;u MXeyOFEzOjJ|MR?=
CA46 M3HxTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITKcpE{NzZxMUKvNlQwPDhibl2= MWe0PEBp NGrzUVBqdmirYnn0d{B1cGViY3XscEBxem:uaX\ldoF1cW:wIHH0JFI1KGGwZDC0PEBvVcLi NEfvUnQzPDB4NEm1NS=>
PMNs M3y1UGZ2dmO2aX;uJGF{e2G7 M4jFUVMxKG6P M3zye|Q5KGh? NGXNc5lqdmirYnn0d{Bm\m[ncn;jfZRwe2m|LDDISGFEKGGldHn2bZR6KGGwZDDSZYMh[WO2aY\peJk> NXjWdGF4OjN7OEi2NVc>
H1299 NVu4do4zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DieFHDqM7:TdMg MkOwNlQwPDhxN{KgbC=> NWr5[4pi\XSqYX7vcC=> MWHpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBi\nSncjCyOIg> M3[xeVI{QTF4NkC5
A549 NXPHe|ZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13JelAvOjVxMD61M|Eh|ryP MorhNlQwPDhxN{KgbC=> MlXZ[ZRp[W6xbB?= MULpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX6gZ49v[2WwdILheIlwdiCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz MW[yN|g3Pzl7MR?=
H1299 NXv2Uld{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zyTVAvOjVxMD61M|Eh|ryP NFvOTVczPC92OD:3NkBp M1;2T4V1cGGwb3y= MWnpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX6gZ49v[2WwdILheIlwdiCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz MlPXNlM5Pjd7OUG=
A549 NVyxe4ppSXCxcITvd4l{KEG|c3H5 MlzUNE42NzFizszN MXi0PEBp MorW[ZRp[W6xbB?= M{L6[Ilv\HWlZYOgZ4VtdCCjcH;weI9{cXN? M3n4SVI{QDZ5OUmx
H1299 NWDmb|hUSXCxcITvd4l{KEG|c3H5 NHfzfnAxNjVxMTFOwG0> M1vnSlQ5KGh? MmXX[ZRp[W6xbB?= MVXpcoR2[2W|IHPlcIwh[XCxcITvd4l{ MYGyN|g3Pzl7MR?=
SUM149PT MoLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\WR|IwPy53L{GwJO69VQ>? NETQXIw1QCCq M4nvc2ROW09? NFX0SINqdmS3Y3XzJINmdGxiZHXheIgh[XRiMjFOwG0hcW6|ZX7zbZRqfmWueR?= NXezUJR3OjN5OUK2N|g>
SUM190PT M4PjO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYC1NE8yODBxMkWwJI5O M3HndlQ5KGh? MUXEUXNQ NE\WN2hqdmS3Y3XzJINmdGxiZHXheIgh[XRiNUCgcm0he2Wwc3n0bZZmdHl? M{jLTVI{Pzl{NkO4
HCT1 NEDy[4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrtfGtyOC5{L{GuNE82NjBizszN NGXLb5IyOi9{ND:zOk81QCCq NH;XZWtqdmS3Y3XzJINmdGxiZHXheIghcW5iY3;uZ4VvfHKjdHnvck0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> MVKyN|c4ODByMB?=
Lovo M3\GPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWCwMlIwOS5yL{WuNEDPxE1? MknmNVIwOjRxM{[vOFghcA>? M{jkdolv\HWlZYOgZ4VtdCCmZXH0bEBqdiClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MW[yN|c4ODByMB?=
AGS MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;HSVAvODF3LUGg{txO NFraV|A4OiCq NXHrV4JzcW6mdXPld{Bk\WyuIHTlZZRpKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NWq0cZZMOjN5NEWwNlQ>
Huh7 NWTBU3lKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4POflEh|ryP NWfMNnJHOjRiaB?= MWLEUXNQ M1:xWZJm\HWlZYOgZYJwfXRiMkClJJZq[WKrbHn0feKh NEj6PWwzOzZ2M{mzNy=>
ECC1 M2HOd2Z2dmO2aX;uJGF{e2G7 MYS1NFAhdk1? M3nZRVUh\A>? MX\EUXNQ MlK2bY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN? MkLzNlM2OzB5Nkm=
HEC1A NYrBRXRyTnWwY4Tpc44hSXO|YYm= NUDvd2lPPTByIH7N Ml;5OUBl MWfEUXNQ NUnyT|ZOcW6mdXPld{BNOUODTdMg[ZhxemW|c3nvckBkdy22cnXheI1mdnRid3n0bEA2NUG8YVO= M1XWZ|I{PTNyN{[5
EN1 NYG4Oot6TnWwY4Tpc44hSXO|YYm= MXW1NFAhdk1? M2Xac|Uh\A>? M16zUGROW09? MUXpcoR2[2W|IFyxR2FOyqCneIDy[ZN{cW:wIHPvMZRz\WG2bXXueEB4cXSqIEWtRZpiSw>? MofmNlM2OzB5Nkm=
MFE296 MWTGeY5kfGmxbjDBd5NigQ>? NYPl[nZvPTByIH7N NGXKcWE2KGR? MnfISG1UVw>? NGmz[IRqdmS3Y3XzJGwyS0GPwrDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?= MXeyN|U{ODd4OR?=
HASMCs MWnGeY5kfGmxbjDBd5NigQ>? NUTGd|FuOC13MECgcm0> NHTteZU3KGR? M1y5ZYRwf25vcnXneYxifGW|IITo[UBmgHC{ZYPzbY9vKG:oIFjBSGMh[W6mIHPlcIwhfmmjYnnsbZR6 NX3GZXY{OjN3MUi0Olc>
U373 NWK3RoVWTnWwY4Tpc44hSXO|YYm= MWOwMlI2NzBwNT:xJO69VQ>? MonjNlQhcA>? NVHtfZA5cW6lcnXhd4V{KFCULVKg[ZhxemW|c3nvckBkdy22cnXheI1mdnRid3n0bEA2SXqjZFO= NIj0NpIzOzR5NEG3NS=>
ARN8  NF7tcGlHfW6ldHnvckBCe3OjeR?= MkfnNE4xPS1{IN88US=> MlXRNlQhcA>? MU\y[YR2[2W|IITo[UBqdmS3Y4Tpc44hd2ZicEWzMYRmeGWwZHXueEBo\W6nczDifUBPfXSuaX6tNy=> MkPqNlM1PzB3NEC=
MCF7 NH3UUVRHfW6ldHnvckBCe3OjeR?= NFXWSWwxNjB3LUKg{txO MkDZNlQhcA>? NUfa[2NvemWmdXPld{B1cGViaX7keYN1cW:wIH;mJJA2Oy2mZYDlcoRmdnRiZ3Xu[ZMh[nliToX0cIlvNTN? MojDNlM1PzB3NEC=
H1299  NIDhV3dHfW6ldHnvckBCe3OjeR?= NGXlWGQxNjN|4pETNeKhyrWP MU[yOE81QCCq M3TieGROW09? NYDGd3NRcW6lcnXhd4V{KEVvY3HkbIVzcW5icILveIVqdiCuZY\lcJMh\G:|ZTDk[ZBmdmSnboTsfS=> NGHTXI8zOzR4MUm3OS=>
H1299 NXLWR3A6TnWwY4Tpc44hSXO|YYm= MnPxNE42KM7:TR?= NGezcm01QCCq MlLQSG1UVw>? NFjxbWxqdmirYnn0d{B1cGVibXnndoF1d3K7IIDveIVvfGmjbDDjc41jcW6nIIfpeIghe2muaXLpcolv NWDDdmRXOjN2NkG5O|U>
H1299 NGDJVVlHfW6ldHnvckBCe3OjeR?= NXy5UJNSOC53IN88US=> NX\LPJU2PDhiaB?= MYXEUXNQ MlK5bY5pcWKrdIOgeIhmKGmwdnHzbZZmdmW|c9MgJINwdWKrbnWge4l1cCC|aXzpZolvcW5? MV[yN|Q3OTl5NR?=
MG-63  M3m4bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;rXVAvOyEQvF2= NHrpTZoyOi17NjDo M2XJNmROW09? MYnpcohq[mm2czD0bIUh[2WubDDndo94fGhiY3:teJJm[XSvZX70JJdqfGhibXX0[o9zdWmw MUGyN|Q2OThzNx?=
LM8 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLP[GQxNjNizszN NH3rUXMyOi17NjDo NFTIXpZFVVOR MUTpcohq[mm2czD0bIUh[2WubDDndo94fGhiY3:teJJm[XSvZX70JJdqfGhibXX0[o9zdWmw M1;5OlI{PDVzOEG3
K562 NY\VNFBrTnWwY4Tpc44hSXO|YYm= NIDOUmMxNjVizszN MoPyNlQhcA>? Mlnk[ZRp[W6xbB?= NYm1dJpT\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5? NX;1cWNGOjN2M{C5OVc>
HEL NW[wdIJ{TnWwY4Tpc44hSXO|YYm= NGG5V3cxNjVizszN MmjLNlQhcA>? MV3leIhidm:u NWizO4Vs\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5? MnrYNlM1OzB7NUe=
HL60 NVXiXVRqSXCxcITvd4l{KEG|c3H5 NXXYTW1XOSEQvF2= M2TvcFI1KGh? MVHpcoR2[2W|wrDj[YxtKGSnYYTo MUWyN|QxODVzOR?=
KG1 Mn7SRZBweHSxc3nzJGF{e2G7 NGLwepYyKM7:TR?= MlPnNlQhcA>? MkfBbY5lfWOnc9MgZ4VtdCCmZXH0bC=> NVzXZnVPOjN2MEC1NVk>
Kazumi MVzBdI9xfG:|aYOgRZN{[Xl? M33pT|Eh|ryP M3LpOFI1KGh? NGTFWlJqdmS3Y3XzxsBk\WyuIHTlZZRp NGLGSW0zOzRyMEWxPS=>
K562 MUTBdI9xfG:|aYOgRZN{[Xl? NEHtfGUyKM7:TR?= MXiyOEBp Mly4bY5lfWOnc9MgZ4VtdCCmZXH0bC=> MnTuNlM1ODB3MUm=
THP1 M1L4ZWFxd3C2b4Ppd{BCe3OjeR?= NGrYbIYyKM7:TR?= NHfHR|YzPCCq NVv0WG1{cW6mdXPld:Kh[2WubDDk[YF1cA>? MnnGNlM1ODB3MUm=
SH-SY5Y MX;GeY5kfGmxbjDBd5NigQ>? MWmyOVAhdk1? MlHiNVYhcA>? M13kOYNp[W6pZYOg[ZhxemW|c3nvckBx[XS2ZYLuJI9nKGenbnXzJIlvfm:udnXkJIlvKGOqb3zld5Rmem:uIIP5cpRp\XOrczygeZB1[WunIHHu[EBm\m[udYi= MkXnNlM{OjZ2MkK=
HEK293 NILt[FJHfW6ldHnvckBCe3OjeR?= M3yyN|EhyrWPwrC= M1X3c|E5KGkEoB?= M37IUolvcGmkaYTzJJRp\SCtYXzpdolvNTdvbXXkbYF1\WRicnXjdpVqfG2nboSgc4Yhe3mwcHjpcIlvNTFiYXfndoVo[XSnczDpcpRwKGGpZ4Lld49u\XN? MXOyN|I5PDh2OB?=
HTK NIT4NIlHfW6ldHnvckBCe3OjeR?= MWe0NFAhdk1? NYLtSWpuPzJiaB?= M3rGWYlvcGmkaYTzJHRITi4QsvMAl2lv\HWlZXSgUZlw\mmkcn;icIF{fCCGaX\m[ZJmdnSrYYTpc44> MnrVNlMzQDRyMEK=
HTK MX7GeY5kfGmxbjDBd5NigQ>? M1fsTFExOC16MEDuUS=> MkLSO|IhcA>? NFzRdWdjdG:la4OgWGdHNc7{4pETTY5lfWOnZDDSU3Mh[W6mIFiyU|LDqEGlY4XteYxifGmxbh?= NXvaRWszOjN{OESwNFI>
Caco-2  NGH3N45HfW6ldHnvckBCe3OjeR?= M3\HO|EhyrWPwrC= NV7BUII5OjRiaB?= MlLV[IVkemWjc3XzJHNGWlRicILveIVqdiCneIDy[ZN{cW:w NVPIcIVsOjNzOUWwO|A>
HeLa NF3WdHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV6xNFAhdk1? MYCyOEBp M3zsVWROW09? MWLJR|UxKG:oIEGwNI5O MmTpNlMyPjV5NEi=
HeLa NYTFR3B[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfSflM1OCCwTR?= NFvvd3A1QCCq MXXEUXNQ MUnJR|UxKG:oIESwcm0> M2PNelI{OTZ3N{S4
HeLa Ml7oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlv4NlAhdk1? Ml[1O|IhcA>? MmHCSG1UVw>? MVTJR|UxKG:oIEKwcm0> M2HLV|I{OTZ3N{S4
HeLa NGfOVYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUH0SFBrOTBxM{CvOVAhdk1? MkjRO|IhcA>? NWjR[I1kTE2VTx?= NX3FXVF{cW6lcnXhd4V{KHSqZTDueY1j\XJib3[gUW1RKCkQlN8ocUkhdG:|czDj[YxteyCmb4PlJIRmeGWwZHXueIx6 NXnrenZpOjNzNkW3OFg>
MDA-MB-231 MnrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYeyfZV5OjVvNECwJI5O MlOzOFghcA>? NX3ZRnV4UUN3MDDv[kAzPjNwMn7NxsA> Mnm3NlMxPTVzOUi=
MCF-7  M4jZeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojTNlUuPDByIH7N NGnaOWQ1QCCq NGTsb3VKSzVyIH;mJFIzOC52bl2= NF\rc24zOzB3NUG5PC=>
ECC-1  NITzcVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkK5NVAxKG6P NFLYVlgzPCCq M1y5dYV1cGGwb3y= MXXpcoNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[gZZBweHSxdHnjJI52[2ynaTD0c{A{PSV? NHLtbo4zOzB{OEiwNy=>
HEC-1A M{HEfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYCxNFAhdk1? M2q5TlI1KGh? M3HCV4V1cGGwb3y= NGLFTG1qdmO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh[XCxcITveIlkKG63Y3zlbUB1dyB|OTW= NYfzZ2dVOjNyMki4NFM>
NHAC-kn NUTYfWtbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWmxNE8yODBxNUCwJI5O MVixNkBp NGrxe|JFVVOR NX;jW|AxUUN3MDDv[kA2ODCwTR?= MVuyN|AyPzh5MR?=
A549 Mlq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\sNVI2OCCwTR?= NW\XfoJGPi15MjDo NWWyZ3pNTE2VTx?= MmDrZ4F2e2W|IHGg[5Jm[XSncjDpcohq[mm2b4L5JIVn\mWldDDjc41jcW6nIIfpeIghXFiWIH;yJIVzdG:2aX7pZi=> M33aS|IzQTl2N{iw
MG-63 M4HXT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXTN|AxKG6P NXzmR2lYOTJiaB?= NHiwd4VFVVOR M2n0fIlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDD0c{A5PiV? NV7DcmE1OjJ5OUmzN|g>
MG-63 NV[1epNyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1mxd|MxOCCwTR?= MYGyOEBp Mlv3SG1UVw>? NEjkZYxqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPjdn Mn72NlI4QTl|M{i=
MG-63 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3uN|AxKG6P MXG0PEBp MXnEUXNQ MmS2bY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIITvJFU3LQ>? MkPBNlI4QTl|M{i=
HL60  MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XXRlE2OC1|NUCgcm0> MnzONlQhcA>? NEm2TmNqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O NXrOTYtHOjJ5NUO3N|k>
U937 MnHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fBOVE2OC1|NUCgcm0> NWHJb5ZXOjRiaB?= NX3pcGhNcW6lcnXhd4V{KGOnbHygZZBweHSxc3nzxsB4cGWwIHPvcoNmdnS{YYTpc45{KGirZ3jldkB1cGGwIEK1NEBvVQ>? MnHUNlI4PTN5M{m=
SCC-6 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HQW|IxOC1|MkCwJI5O NGXNOIMyOi9{ND:0PEBp MWrEUXNQ MV\pcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDv[kBUS0NvNjDj[YxteyCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? Mn3ENlI2PTJ|MkG=
U87  MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlP2NVAxNTNyMDDu[y=> NV\rVoFOOjRiaB?= MWjpcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gO|ImKCCjdDCyNFBv\w>? M4LybVIzOjdyOES5
K562 MVvGeY5kfGmxbjDBd5NigQ>? Mmr3NUDPxE1? NXPaWmFMOTJiaB?= MV7EUXNQ M{nOfIVvcGGwY3XzJJRp\SCneIDy[ZN{cW:wIH;mJHJWVlh|IHnu[JVk\WRiYomgOU1igmFvQ3TS M2XnclIzOTd7MUm4
Reh Mnq5SpVv[3Srb36gRZN{[Xl? NISxUJUxNjNxMTFOwG0> NVT4T2M4OTJiaB?= NVe0WVlSTE2VTx?= M3jxe4VvcGGwY3XzJJRp\SCneIDy[ZN{cW:wIH;mJHJWVlh|IHnu[JVk\WRiYomgOU1igmFvQ3TS NVridoFXOjJzN{mxPVg>

... Click to View More Cell Line Experimental Data

In vivo試験 Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]
臨床試験
特集

プロトコル (参考用のみ)

キナーゼアッセイ:

[1]

In vitro HDAC activity Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.

細胞アッセイ:

[1]

細胞株 MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
濃度 Dissolved in absolute ethanol, final concentrations ~10 μM
反応時間 96 hours
実験の流れ

Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.

動物実験:

[1]

動物モデル Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
製剤 Dissolved in DMSO
投薬量 ~5 mg/kg/day
投与方法 Injection s.c.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDogMonkeyBaboon
Weight (kg)0.020.151.80.40.0810312
Body Surface Area (m2)0.0070.0250.150.050.020.50.240.6
Km factor361285201220
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

参考

化学情報

Download Trichostatin A (TSA) SDF
分子量 302.4
化学式

C17H22N2O3

CAS No. 58880-19-6
保管 2年-20℃
6月-80℃in solvent
別名 N/A
溶解度 (25°C) * In vitro DMSO 23 mg/mL (76.05 mM)
<1 mg/mL (<1 mM)
エタノール <1 mg/mL (<1 mM)
In vivo 1% DMSO+30% polyethylene glycol+1% Tween 80, pH 4 6 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
化学名 (R,2E,4E)-6-(4-(dimethylamino)benzoyl)-N-hydroxy-4-methylhepta-2,4-dienamide

カスタマーフィードバック (4)


Click to enlarge
Rating
Source Epigenetics, 2012, 7(10):1161-72. Trichostatin A (TSA) purchased from Selleck
Method Immunofluorescence staining
Cell Lines H1299 cells
Concentrations 0.5 uM
Incubation Time 48 h
Results To identify whether these G2/M fractions of cells were arrested at G2 phase or undergoing a mitotic arrest, we analyzed 48 h treated cells for the expression of TSA and Saha, p-histone H3 Ser 10 and p-MPM-2Ser /Thr. As indicated by decreased nuclear expression of p-histone H3 Ser 10, quantitative analysis of immunofluorescence (IF) data showed a significant decrease in mitotic index (% of cells in mitotic phase) by combinatorial treatments. This was further corroborated by decreased expression of MPM-2 reactive structured components of the mitotic apparatus that are required for both onset and completion of M-phase.

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Rating
Source Biochem Biophys Res Commun, 2014, 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines HCT116 p53 null cells
Concentrations 1 uM
Incubation Time 24 h
Results TSA causes ER stress and increase the expression of ATF4, ATF3, CHOP.

Click to enlarge
Rating
Source Biochem Biophys Res Commun, 2014, 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines HCT116 p53 null cells
Concentrations 2 uM
Incubation Time 24 h
Results Knockdown of ATF3 suppressed the induction of DR5 protein and knockdown of ATF4 suppressed the induction of ATF3, CHOP and DR5 proteins by these HDACIs(TSA, M344, MS-275).

Click to enlarge
Rating
Source 2010, Dr. Zhang of Tianjin Medical University. Trichostatin A (TSA) purchased from Selleck
Method Western blot
Cell Lines MDA-MB-231 cells
Concentrations 0-20 μM
Incubation Time
Results Reduction of Histone H3 acetylation in MDA-MB-231 cells treated with Trichostatin A was observed.

文献中の引用 (20)

技術サポート&よくある質問(FAQ)

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Tags: Trichostatin A (TSA)を買う | Trichostatin A (TSA)供給者 | Trichostatin A (TSA)を購入する | Trichostatin A (TSA)費用 | Trichostatin A (TSA)生産者 | オーダーTrichostatin A (TSA) | Trichostatin A (TSA)代理店
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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