LY294002

製品コードS1105 別名:SF 1101, NSC 697286

LY294002化学構造

分子量(MW):307.34

LY294002は初めての周知されたPI3Kα/δ/βを抑制する小分子で、無細胞試験でIC50値が0.5 μM/0.57 μM/0.97 μM に分かれることですが、溶液にの状態はWortmanninにの状態よりもっと安定になりって、自食体の形成も遮断できます。

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文献中の引用(70)

カスタマーフィードバック(13)

  • Type I IFNs induce ISG expression in a serine phosphorylation-independent manner. Cells were treated with kinase inhibitors (SB203580, rottlerin, Ly294002, PD98059, and SP600125) before stimulation with IFN-a and IFN-b for 6 hours. Cell lysates were used in western blotting and probed for serine phosphorylation.The relative amounts of pSTAT1 S727 in (up-graph) were quantied and normalized to an untreated control (below-graph).

    Hepatology 2014 59(4), 1262-72. LY294002 purchased from Selleck.

    different signal-inhibitory profile by applying 120 nmol/L BGT226, 20 μmol/L LY294002, or 100 nmol/L rapamycin in FaDu cells.

    Clin Cancer Res 2011 17, 7116-7126. LY294002 purchased from Selleck.

  • Stimulation response of p37d-expressing cells. (c) Western blot of 5 min serum stimulated HEK-293 cells stably expressing p37d, p110d or control, with the presence of inhibitors as indicated at the top.

    Oncogene 2012 31, 3277–3286. LY294002 purchased from Selleck.

    PTEN transfection or inhibition of the PI3K-AKT and IGF-1R signaling pathways increases the trastuzumab sensitivity of NCI-N87/TR cells. After treating the cells with a PI3K inhibitor (LY294002), the expression of AKT and P-AKT proteins was assessed by Western blotting. Tubulin expression indicated equal loading. All gels run under the same experimental conditions and the experiments were repeated 3 times. The representative images were cropped and shown. Control: cells without any treatment.

    Sci Rep 2015 5, 11634. LY294002 purchased from Selleck.

  • SCC61 or Detroit 562 xenografted tumors respond to treatment by PI3K inhibitor, LY294002 alone, or in combination with gefitinib. % tumor growth is shown. By two-way ANOVA, the effect of treatment and time is considered significant;

    Mol Oncol 2013 7, 359-68. LY294002 purchased from Selleck.

    Inhibition of EGFR and PI3K/AKT pathway has additive effects in SCCHN cell lines with constitutively active PIK3CA mutations, while removing negative regulation of PI3K/AKT pathway increases resistance in sensitive SCCHN cell line. A, results from MTT assays on cells treated with PI3K inhibitor, LY294002, and gefitinib.

    Mol Oncol 2013 7, 359-68. LY294002 purchased from Selleck.

  • Immunofluorescence and flow cytometric analyses of the translocation of ABCG2 in MCF-7 FLV1000 cells before and after treatment with the PI3K inhibitor LY294002. Confocal microscopy of MCF-7 FLV1000 cells after a 16-h treatment with 2 uM or 4 uM of LY294002 was performed as described in Figure.

    Br J Pharmacol 2013 70(5), 1137-51. LY294002 purchased from Selleck.

    IGF1 increases apical fibronectin in late stage blastocysts. Representative confocal images of blastocysts stained for fibronectin (Green) and DAPI (Blue). Day 5 blastocysts were cultured in SS medium for 24 (A and B), 48 (C and D) or 72 h (E-H) in the absence (A, C and E) or presence (B, D and F) of 10 ng/ml IGF1 or in the presence of a PI3 Kinase inhibitor (5 uM LY294002 + 10 ng/ml IGF1) (G) or in the presence of 10 ng/ml IGF1, attached to a coverslip (H). Scale bars represent 20 mm. Red line indicates location of coverslip. Arrow indicates location of the inner cell mass (ICM). The following total number of blastocysts were analysed, from at least 3 experimental repeats in each treatment; 24 in the SS group (24 h), 21 in SS plus IGF1 (24 h), 26 in SS (48 h), 30 in SS plus IGF1 (48 h), 40 in SS (72 h), 59 in SS plus IGF1 (72 h), 13 in SS plus IGF1 + LY294002 (48 h) and 15 blastocysts were analysed that were attached to a coverslip (72 h).

    Hum Reprod 2015 30(2), 284-98. LY294002 purchased from Selleck.

  • Inhibition of PI3K, ERK and mTOR prevents the activation of S6K1 and S6 induced by suppression of PKD1 activity. A549 cells were incubated in the absence (-) or presence of either 5 uM Kb or 5 uM Kb and 20 uM LY294002 or 5 uM Kb and 10 uM BKM120 (as indicated) for 1 h prior to stimulation of cells with 50 nM PMA for 30 min and 1 h.

    Int J Biochem Cell Biol 2015 60, 34-42. LY294002 purchased from Selleck.

    We treated all of drugs in T47D which has a PI3KCA H1044R mutation with the concentration shown below for 1 hour and performed western blot analysis using antibodies to phospho-AKT(SERINE 472), and total AKT.

     

     

    2010 Saraswati Sukumar of Johns Hopkins University School of Medicine. LY294002 purchased from Selleck.

  • T47Dcells were pretreated with 100 ng/ml EGF for 20 min and then treated with the indicated concentrations of LY294002 for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. LY294002 purchased from Selleck.

    Comparative effects of inhibitors by immunofluorescence microscopy study. Confluent HC11 cells were grown on poly-L-lysine-coated glass coverslips (immunofluorescence) and on plastic plates (biochemical control) and then treated with inhibitors according to the standard procedure. Upper part: the biochemical action of the inhibitors was tested to validate the immunofluorescence results. Cellular proteins were analyzed by SDS-PAGE and the immunoblots were successively probed with anti-ADRP, anti-β-casein, and anti- β-actin antibodies and their respective HRP-conjugated secondary antibodies. Each experimental condition was performed in duplicate. Lower part: cells were fixed, permeabilized and subjected to immunofluorescence microscopy using antiserum against ADRP and TRITC-conjugated secondary antibody (red).

    Biochim Biophys Acta 2012 1823(5), 987-96. LY294002 purchased from Selleck.

  • Comparative effects of wortmannin and LY294002 on ADRP and β -casein synthesis. Con fluent HC11 cells were treated according to the standard procedure in the absence or presence of 4 ng/ml EGF either with or without 100 nM wortmannin or 50μM LY294002. Cellular proteins were analyzed by SDS-PAGE and immunoblot successively probed with anti-ADRP, anti-β-casein, anti-β -actin antibodies and their respective HRP-conjugated secondary antibodies. ECL signals were quantified by densitometry and normalized to β-actin. Each experimental condition was performed in triplicate. Percentages of inhibition for both inhibitor and hormonal treatments are indicated in the text.

    Biochim Biophys Acta 2012 1823(5), 987-96. LY294002 purchased from Selleck.

製品安全説明書

PI3K阻害剤の選択性比較

生物活性

製品説明 LY294002は初めての周知されたPI3Kα/δ/βを抑制する小分子で、無細胞試験でIC50値が0.5 μM/0.57 μM/0.97 μM に分かれることですが、溶液にの状態はWortmanninにの状態よりもっと安定になりって、自食体の形成も遮断できます。
靶点
p110α [1]
(Cell-free assay)
p110δ [1]
(Cell-free assay)
p110β [1]
(Cell-free assay)
0.5 μM 0.57 μM 0.97 μM
In vitro試験

LY294002 inactivates Akt/PKB, consequently inhibiting cell proliferation and inducing apoptosis. LY294002 demonstrates a remarkable growth-inhibitory and apoptosis-inducing effect in these colon cancer cell lines, with decreased expression of phosphorylated Akt (Ser473). [2]LY294002 induces marked nuclear pyknosis and diminished cytoplasmic volume in the tumor cells. Thus, LY294002 markedly inhibits ovarian cancer cell proliferation in vitro. LY294002 induces specific G1 arrest in cell growth, leading to almost complete inhibition of melanoma cell proliferation and partial inhibition of MG-63 (osteosarcoma cell line) proliferation. The effect of LY294002 on cell cycle progression may provide insights into a possible link between the PI3K activation pathway and cancer cell cycle regulation. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-231 cells NGXtPI9E\WyuIHnueoF{cW:wIHHzd4F6 NWXEN21DOjRiaB?= MX;Jcohq[mm2aX;uJI9nKGWyaYTo[Yxq[WxiZ4Lve5RpKG[jY4Tvdk1qdmS3Y3XkJINmdGxibXnndoF1cW:wIH;mJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNicILlMYlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHPlcIwhcW64YYPpc44h[XO|YYmsJGlEPTB;MD6zPEDPxE1w Mof5NlI5ODRzMEi=
Sf21 cells M{\ydWZ2dmO2aX;uJIF{e2G7 NEXUT|BKdmirYnn0bY9vKG:oIH3veZNmKHerbHSgeJlx\SCSSUPLZYxxcGFiZYjwdoV{e2WmIHnuJHNnOjFiY3XscJMh[29vZYjwdoV{e2mwZzDOMZRmem2rbnHsJGhqey22YXfn[YQhcHWvYX6gdFg2[SC3c3nu[{BNNWGucHjhMZBpd3OyaHH0bYR6dGmwb4PpeI9tKHO3YoP0doF1\SCkeTDUUGMh[mG|ZXSgdIhwe3Cqb4KgbY1i\2mwZzygTWM2OD1yLkWg{txO M1P5SVIzPTJyNkOw
Sf21 cells MoPYSpVv[3Srb36gZZN{[Xl? Mn\lTY5pcWKrdHnvckBw\iCvb4Xz[UBz\WOxbXLpcoFvfCCSSUPLZYxxcGFiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{NWmwZnXjeIVlKFOoMkGgZ4VtdHNuIFnDOVA:OC53IN88UU4> MWWxPVc1QDJ4OR?=
Sf9 cells MXLGcJVwemW|Y3XueEBxd2yjcnn6ZZRqd25iYYPzZZk> MWPJcohq[mm2aX;uJI9nKGi3bXHuJHBKO0ujbIDoZUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHL5JIZtfW:{ZYPj[Y51KHCxbHHybZpifGmxbjDhd5NigSxiSVO1NF0xNjV3IN88UU4> M4TicFIyOTJzNkOx
THP1 cells MWDGeY5kfGmxbjDhd5NigQ>? MoHiTY5pcWKrdHnvckBw\iCVQ1[xMYlv\HWlZXSgVGtDN0GtdDDwbI9{eGixconsZZRqd25iaX6gWGhROSClZXzsd{whUUN3ME2xMlAyKM7:TT6= M1zGXFE3Pzh7N{Sy
human PC3 cells NHTzbnBHfW6ldHnvckBie3OjeR?= NXXEZ2lwOzBibXnu NFjV[oZKdmirYnn0bY9vKG:oIGDJN2shcW5iaIXtZY4hWEN|IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJGFMXCCyaH;zdIhwenmuYYTpc44h[XRic3XybY5mKDR5MzDh[pRmeiB|MDDtbY5{KGK7IFXMTXNCNCCLQ{WwQVEvO87:TT6= MYqyN|QyODByNR?=
HeLa (human carcinoma) cells. NH7ve2FHfW6ldHnvckBie3OjeR?= MYrJckB3cXS{bzDpcohq[mm2aX;uJI9nKESQQT3k[ZBmdmSnboSgdJJwfGWrbjDrbY5ie2VqRF7BMXBMMSCocn;tJGhmVGFiKHj1cYFvKGOjcnPpco9u[SliY3XscJMtKEmFNUC9NU41KM7:TT6= M{\GTlE2PjV6OEew
THP1 cells NHj1dodHfW6ldHnvckBie3OjeR?= MkL3TY5pcWKrdHnvckBw\iCPQ2CxMYlv\HWlZXSgVGtDN0GtdDDwbI9{eGixconsZZRqd25iaX6gWGhROSClZXzsd{whUUN3ME2xMlY2KM7:TT6= M2XIV|E3Pzh7N{Sy
HeLa cells MXrGeY5kfGmxbjDhd5NigQ>? MnOzTY5pcWKrdHnvckBw\iCvVF;SJJBzd3SnaX6gbZNwdGG2ZXSg[pJwdSCKZVzhJINmdGy|LDDJR|UxRTJwNTFOwG0v NXvyO|VLOTV4NUi4O|A>
human MCF7 cells NVzzSYZyS3m2b4TvfIlkyqCjc4PhfS=> MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIEKwJO69VSClaHzvdo9yfWmwZTDifUBUWkJiYYPzZZktKEeLNUC9Nk43OyEQvF2u MmKwNVg3QTF6OUS=
HeLa cells NX3sbmtJTnWwY4Tpc44h[XO|YYm= NUe5OYdbUW6qaXLpeIlwdiCxZjDBXE04PTB|IHLpcoRqdmdidH:gdoVkd22kaX7hcpQhWGytMzDlfJBz\XO|ZXSgbY4hUGWOYTDj[YxteyCkeTDX[ZN1\XKwIHLsc5QtKEmFNU2zJO69VS5? NULvbIE1OTdzM{WyOFg>
human MCF7 cells MkHFR5l1d3SxeHnjxsBie3OjeR?= NUXQNmtkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCrbjDwdoV{\W6lZTDv[kAyOCC3TTDjbIxwem:zdXnu[UBjgSCVUlKgZZN{[XluIFfJOVA:Oy5yODFOwG0v MYixPFY6OTh7NB?=
human MCF7 cells NF3OWmhEgXSxdH;4bYPDqGG|c3H5 NYm5XYVJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCkeTDTVmIh[XO|YYmsJGdKRTNwMU[g{txONg>? NHHDXHAyQDZ7MUi5OC=>
human MDA-MB-231 cells NGrsWppEgXSxdH;4bYPDqGG|c3H5 NGnZblBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIlvKHC{ZYPlcoNmKG:oIEKwJO69VSClaHzvdo9yfWmwZTDifUBUWkJiYYPzZZktKEeLPUOuN|Ih|ryPLh?= Mlu4NVg3QTF6OUS=
human MDA-MB-231 cells NYLxXXFxS3m2b4TvfIlkyqCjc4PhfS=> M3rQVGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiMUCgeW0h[2iub4LvdZVqdmViYomgV3JDKGG|c3H5MEBIUTVyPUSuN|Uh|ryPLh?= NF;5TGIyQDZ7MUi5OC=>
human MDA-MB-468 cells NVfpTJc1S3m2b4TvfIlkyqCjc4PhfS=> MlHPR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUS2PEBk\WyuczDpckBxemW|ZX7j[UBw\iB{MDD1UUBkcGyxcn;xeYlv\SCkeTDTVmIh[XO|YYpvwKwhUUN3ME20Mlc3KM7:TT6= MWixPFY6OTh7NB?=
human HCT116 cells M2fLWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4Ppc|Q5KGh? NYPIPW5zT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUG2JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKFCLM1vhcJBp[SCKMUC0O3IhdXW2YX70JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NT6zJO69VQ>? NIDuSGszOjJzMkeyNS=>
human HCT116 cells MmfjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3ewfVQ5KGh? NXzRd4xYT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PS56IN88UU4> MmTkNlIzOTJ5MkG=
HeLa cells MnXTRolv\GmwZzDh[oZqdmm2eTDhd5NigQ>? MkG4Rolv\GmwZzDh[oZqdmm2eTDmc5IhWEl|LXvpcoF{\SCrc3;sZZRm\CCocn;tJGhmVGFiY3XscJMtKEurPU[g{txO M4PKWVE2PjV6OEew
human HCT116 cells NX3STXo1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoD3OFghcA>? MlLaS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGNVOTF4IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJHBKO0ujbIDoZUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPU[uO{DPxE1w M1:wPVIzOjF{N{Kx
MDA-MB-231 cells NIHaZWhEgXSxdH;4bYPDqGG|c3H5 NHnZSopEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF03NjdzIN88UU4> NH3MdHYyQDZ7MUi5OC=>
Sf9 cells MW\GeY5kfGmxbjDhd5NigQ>? MX\Jcohq[mm2aX;uJI9nKEONMjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{NCCLQ{WwQVYvQSEQvF2u MY[xPFMzOTdzNh?=
human U87MG cells NX7RdVF4TnWwY4Tpc44h[XO|YYm= NHTTSIRKdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTDpckBpfW2jbjDVPFdOTyClZXzsd{BjgSCHTFnTRUwhUUN3ME24MlEh|ryPLh?= NXK3Row4OTh|NEW2NFk>
human MDA-MB-468 cells NX\1ZZJOS3m2b4TvfIlkyqCjc4PhfS=> NGfwd|ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFY5KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIEGwJJVOKGOqbH;yc5F2cW6nIHL5JHNTSiCjc4PhfUwhT0l3ME24MlIh|ryPLh?= NVXXN|lZOTh4OUG4PVQ>
human A375 cells NYfzO2hkWHKxbHnm[ZJifGmxbjDhd5NigQ>? M{LlXFQ3KGh? NVHx[HB3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIFnfGW{IES2JIhzeyxiSVO1NF05NjRizszNMi=> M3O5S|E4ODR7MkS4
human A375 cells MXLQdo9tcW[ncnH0bY9vKGG|c3H5 NF3sbFNKdmirYnn0bY9vKG:oIIPldpVuNWmwZIXj[YQheHKxbHnm[ZJifGmxbjDv[kBpfW2jbjDBN|c2KGOnbHzzMEBKSzVyPUiuOEDPxE1w M4PvSlE4PjBzN{O5
human HL60 cells M4\1ZWN6fG:2b4jpZ:Kh[XO|YYm= NYi0c|A5PzJiaB?= M3faTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME25Mlk1KM7:TT6= NW[0cHZ{OjJ2OEC4OVE>
mouse Raw264 macrophage NFTWZYlHfW6ldHnvckBie3OjeR?= M1\tXmlvcGmkaYTpc44hd2ZiQ{XhMY1m\GmjdHXkJHBMSi:Da4SgdIhwe3Cqb4L5cIF1cW:wIHnuJI1wfXOnIGLhe|I3PCCvYXPyc5Bp[WenLDDJR|UxRTFyIN88UU4> MnjMNVY4QDl5NEK=
Sf9 cells NW\rR3VtTnWwY4Tpc44h[XO|YYm= MUPJcohq[mm2aX;uJI9nKEircz30ZYdo\WRiYn;2bY5mKFCLM1ug[ZhxemW|c3XkJIlvKFOoOTDj[YxteyxiSVO1NF0yOCEQvF2u NUH0W3VvOTB7OUizOVE>
human MDA-MB-468 cells MYnDfZRwfG:6aXRCpIF{e2G7 NX35ToVxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BjgSCVUlKgZZN{[XluIFnDOVA:OTBwNDFOwG0v M2HZPVE5PjlzOEm0
human PC3 cell Mo\zVJJwdGmoZYLheIlwdiCjc4PhfS=> M3vkVFczKGh? NV7FbndiUW6qaXLpeIlwdiCxZjDoeY1idiCSQ{OgZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDzdIVkfHKxcHjveI9u\XS{aXOgZY5idHm|aYOsJGlEPTB;MUKuNUDPxE1w MkL1NlM1OTByMEW=
human HL60 cells MnzFVJJwdGmoZYLheIlwdiCjc4PhfS=> NVnJZopzPzJiaB?= MljhRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYXLzc5Jj[W6lZTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF6LkSzJO69VS5? MVmyOVY6Ozd6Nx?=
human BT474 cell MYfQdo9tcW[ncnH0bY9vKGG|c3H5 NIjnbZI4OiCq MVPJcohq[mm2aX;uJI9nKGi3bXHuJGJVPDd2IHPlcIwheHKxbHnm[ZJifGmxbjDh[pRmeiB5MjDodpMh[nlic4DlZ5Rzd3Cqb4TvcYV1emmlIHHuZYx6e2m|78{MJGlEPTB;MkCuO{DPxE1w NI\sbHIzOzRzMECwOS=>
THP1 cells NGXWNZhHfW6ldHnvckBie3OjeR?= NYqzfIdXUW6qaXLpeIlwdiCxZjDNR3AyNWmwZIXj[YQh[2inbX;0ZZhqeyCxZjDUTHAyKGOnbHzzMEBKSzVyPUO3JO69VS5? NUDUdGpmOTZ5OEm3OFI>
human 184B5 cells NUDuclFiS3m2b4TvfIlkyqCjc4PhfS=> M2HEPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFE5PEJ3IHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD1|OT6zO{DPxE1w MXOxPFY6OTh7NB?=
human KB cells MYfDfZRwfG:6aXRCpIF{e2G7 MVe3NkBp NUnFOmRUS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20OE44PiEQvF2u M364b|IyQTR3MkWw
mouse RAW264.7 cells NV3ye3lVTnWwY4Tpc44h[XO|YYm= MknFNlUh|ryP MX6yNEBp NVu5cGR[UW6qaXLpeIlwdiCxZjDQTVNMN0GNVDDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIHnOU3MheHKxdHXpckBmgHC{ZYPzbY9vKGG2IEK1JO69VSCjZoTldkAzOCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| M{G2ZlI1Ojl7NkG2
human A2780 cells MWfBdI9xfG:|aYOgZZN{[Xl? MmrJNkDPxE1? NVjPeJVKOTJiaB?= NX;ZfJJ6UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBkcXOybHH0bY4uemW|aYP0ZY51KGi3bXHuJGEzPzhyIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJJBpd3OyaH;yfYxifGWmIFHreEBt\X[nbDDheEAzKHWPIHHmeIVzKDF{IHjyd{BjgSCrbX31co9jdG:2IHHuZYx6e2m|Lh?= NYPjUXhnOTd4OESwNVg>
human SW480 cells M2POW2Z2dmO2aX;uJIF{e2G7 NHzWRVQzOCEQvF2= M3O3[|I1KGh? M1qwXmRm[3KnYYPlJIlvKHO3co\peolvKGW6cILld5Nqd25iaX6gbJVu[W5iU2e0PFAh[2WubIOgZZQhOjBidV2gZYZ1\XJiMkSgbJJ{KGK7IHntcZVvd2Kub4SgZY5idHm|aYO= NV;pZWhvOTd2N{K5OlI>
mouse mast cells NGnWZ4lHfW6ldHnvckBie3OjeR?= NUn1SXZuOjBizszN MkHGTY5pcWKrdHnvckBw\iCVQ1[tbY5lfWOnZDDQT2IwSWu2IIDoc5NxcG:{eXzheIlwdiCrbjDtc5V{\SCvYYP0JINmdGy|IHH0JFIxKM7:TR?= NUO1TmlHOTZ5OEm3OFI>
human BJ cells MVvGeY5kfGmxbjDhd5NigQ>? NVewOFFoOjVizszN MkDXNkBp MUPJcohq[mm2aX;uJI9nKFCLM1ugd4lodmGuaX7nJIlvKFCGR1[td5RqdXWuYYTl[EB{\XK3bTDzeIFzfmWmIHj1cYFvKEKMIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIgheGyjc33p[EBGT0[SL2DIJIRwdWGrbjDv[kBCc3RiYYPz[ZN{\WRiYYOgd5VxeHKnc4Ppc44hd2ZiUFitSWdHWCC{ZXTpd5RzcWK3dHnvckB1dyCybHHzcYEhdWWvYoLhcoUh[XRiMkWgeW0hfHKnYYTl[EAzKGi{czDi[YZwemViUFTHSkBkcGGubHXu[4Uh[nliY3;u[o9k[WxibXnjdo9{[2:yeR?= M4LJOVE3PzZ5MEi1
human IGROV1 cells MVHGeY5kfGmxbjDhd5NigQ>? NGHiXVczPC92ODDo NYe5bJZ7UW6qaXLpeIlwdiCxZjDQTVNMN0GtdDDpckBpfW2jbjDJS3JQXjFiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gdIhwe3Cqb4L5cIF1\WRiQVvUJIxmfmWuIHH0JFEhfG9iNTD0bY1meyCLQ{WwJIFnfGW{IEK0JJRwKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= M{TEVVIyOjF4MUWx
human IGROV1 cells M13HR2Z2dmO2aX;uJIF{e2G7 MVSyOE81QCCq Mn;KTY5pcWKrdHnvckBw\iCSSUPLM2FsfCCrbjDoeY1idiCLR2LPWlEh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY4njcIlvKERzIHzleoVtKGG2IEGgeI8hPSC2aX3ld{BKSzVyIHHmeIVzKDJ2IITvJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MoLxNlEzOTZzNUG=
rat RBL2H3 cells M4C2eGZ2dmO2aX;uJIF{e2G7 NUC1XGZHUW6qaXLpeIlwdiCxZjDBNlMyQDdvaX7keYNm\CCmZXfyZY52dGG2aX;uJIlvKHKjdDDSRmwzUDNiY3XscJM> NF3ZSHkyQDN{OEexOi=>
human BJ cells NWW4OoEyTnWwY4Tpc44h[XO|YYm= MmDSNlUh|ryP M4qyNlIhcA>? NFi0dZlC[3SrdnH0bY9vKG:oIFHreEBxcG:|cHjvdplt[XSrb36gZZQhXGi{M{C4JIlvKFCGR1[td5RqdXWuYYTl[EB{\XK3bTDzeIFzfmWmIHj1cYFvKEKMIHPlcIx{KGG2IEK1JJVOKHS{ZXH0[YQhOiCqcoOgZoVnd3KnIGDES2Yh[2ijbHzlcodmKGK7IHntcZVvd2Kub4T0bY5o MYixOlc3PzB6NR?=
human HCT116 cells NYPxU2huTnWwY4Tpc44h[XO|YYm= M17WfFExKM7:TR?= M1jES|ExKG2rbh?= MYnJcohq[mm2aX;uJI9nKFCLM1vhcJBp[SCrbjDoeY1idiCKQ2SxNVYh[2WubIOgeZNqdmdiW{OyVH1CXFBiYYSgNVAhfU1iYX\0[ZIhOTBibXnudy=> NGPPXpozOjJzMkeyNS=>
human HCT116 cells M{DqdWZ2dmO2aX;uJIF{e2G7 M4P5PVExKM7:TR?= MmnvNVAhdWmw MVTJcohq[mm2aX;uJI9nKFCLM1vhcJBp[SCKMUC0O3IhdXW2YX70JIlvKGi3bXHuJGhEXDFzNjDj[YxteyC3c3nu[{BcOzKSXVHUVEBifCBzMDD1UUBi\nSncjCxNEBucW6| NYLsS|BQOjJ{MUK3NlE>
human HCT116 cells NGfGO4dHfW6ldHnvckBie3OjeR?= NILpTm4yOCEQvF2= MnrUTY5pcWKrdHnvckBw\iCSSUPLZYxxcGFiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gRYt1KFOnckS3N{BxcG:|cHjvdplt[XSrb36gZZQhOTBidV2gZpkhcW2vdX7vZoxwfCCjbnHsfZNqew>? MV[yNlIyOjd{MR?=
human HCT116 cells M1Hh[2Z2dmO2aX;uJIF{e2G7 NFfLcZQyOCEQvF2= NVzldlhNUW6qaXLpeIlwdiCxZjDQTVNM[WyyaHGgTFExPDeUIH31eIFvfCCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDBb5QhW2W{NEezJJBpd3OyaH;yfYxifGmxbjDheEAyOCC3TTDifUBqdW23bn;icI91KGGwYXz5d4l{ M3m2VFIzOjF{N{Kx
human PC3 cells NIn5fWdHfW6ldHnvckBie3OjeR?= M1nPUlUxKM7:TR?= NVzBOJB1QCCq MnrMTY5lfWO2aX;uJI9nKHB{MTDwdo91\WmwIHX4dJJme3Orb36gbY4hcHWvYX6gVGM{KGOnbHzzJIF1KDVyIIXNJIlv[3WkYYTl[EBnd3JiODDodpMh[nliV3XzeIVzdiCkbH;0eIlv\yC{ZXzheIl3\SC2bzD1cpRz\WG2ZXSgZ49vfHKxbB?= NGXBR5kzOjl7OES3Ni=>
human LNCAP cells MWrGeY5kfGmxbjDhd5NigQ>? NVP2N2Q{OC5zLUOwJO69VQ>? MlW0N|AhdWmw MnrSTY5pcWKrdHnvckBw\iCDa4SgdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIFzOR2FRKGOnbHzzJIF1KDBwMTD0c{A{OCC3TTDh[pRmeiB|MDDtbY5{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MUmyNlg{OjNzNh?=
human LNCAP cells NIKwNIJHfW6ldHnvckBie3OjeR?= NFXWflAxNjFvM{Cg{txO M{HHR|I1KGh? MUnEc5dvemWpdXzheIlwdiCxZjDzeZJ3cX[rbjDlfJBz\XO|aX;uJIlvKGi3bXHuJGxPS0GSIHPlcIx{KGG2IECuNUB1dyB|MDD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ NWK5[Y5iOjJ6M{KzNVY>
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human H1299 cells M1i3OWZ2dmO2aX;uJIF{e2G7 NXXLOnM6OC5zLUOwJO69VQ>? MnX5NlQhcA>? NUPIOVltTG:5bjDy[Yd2dGG2aX;uJI9nKGOLQWCyJIV5eHKnc4Ppc44hcW5iaIXtZY4hUDF{OUmgZ4VtdHNiYYSgNE4yKHSxIEOwJJVOKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NFX0[WwzOjZ{MkC2PS=>
human H1299 cells NWC5[oo5TnWwY4Tpc44h[XO|YYm= M4S0XFAvOS1|MDFOwG0> MVqyOEBp NUC0dWI1TG:5bjDy[Yd2dGG2aX;uJI9nKGOLQWCyJIV5eHKnc4Ppc44hcW5iaIXtZY4hUDF{OUmgZ4VtdHNiYYSgNE4yKHSxIEOwJJVOKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXNiaX6gdJJme2WwY3Wgc4YhOTByIIXnM41tKFSUQVnMJIFv\CCSSUPLJIlvcGmkaYTvdkBNYTJ7NECwNi=> NEmw[YYzOjZ{MkC2PS=>
human H1299 cells MmXzSpVv[3Srb36gZZN{[Xl? M1LBRVAvOS1|MDFOwG0> NVfaNmI6OjRiaB?= NXXEcIozUW6mdXP0bY9vKG:oIFTSOUBmgHC{ZYPzbY9vKGmwIHj1cYFvKEhzMkm5JINmdGy|IHH0JFAvOSC2bzCzNEB2VSCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MYWyNlYzOjB4OR?=
human H1299 cells MUHGeY5kfGmxbjDhd5NigQ>? M4HyeFAvOS1|MDFOwG0> MUiyOEBp M1jLWmlv\HWldHnvckBw\iCMTlutcYVlcWG2ZXSgR2hQWCCneIDy[ZN{cW:wIHnuJIh2dWGwIFixNlk6KGOnbHzzJIF1KDBwMTD0c{A{OCC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MmLuNlI3OjJyNkm=
HEK293 cells M2DUcmZ2dmO2aX;uJIF{e2G7 MmOxNU42KGh? MU\EbZNxdGGlZX3lcpQhd2ZiW{PIYWxUTCCocn;tJIh2dWGwIEXIWFYhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bi\nSncjCxMlUhcHK|IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70bY5o MlzVNlI2OzdzNUO=
human U251HRE cells NXy1VXVXTnWwY4Tpc44h[XO|YYm= M2jub2lvcGmkaYTpc44hd2ZiaInwc5hq[S2rbnT1Z4VlKEiLRkGgZYN1cX[jdHnvckBqdiCqdX3hckBWOjVzSGLFJINmdGy|IHL5JINmdGxiYnHz[YQhemWyb4L0[ZIh\2WwZTDhd5NigQ>? M4H4TlE5PTBzNkCx
human HCT116 cells MnjLSpVv[3Srb36gZZN{[Xl? MmXwNVAh|ryP MoL3TY5pcWKrdHnvckBw\iCDa4Sg[ZhxemW|c3nvckBqdiCqdX3hckBJS1RzMU[gZ4VtdHNiYYSgNVAhfU1iYomgbY1ufW6xYnzveEBidmGueYPpdy=> NULjVXNIOjJ{MUK3NlE>
Sf9 cells MWjmcJVwemW|Y3XueEBxd2yjcnn6ZZRqd25iYYPzZZk> MVfJcohq[mm2aX;uJI9nKGi3bXHuJHBKO0upYX3tZUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHL5JIZtfW:{ZYPj[Y51KHCxbHHybZpifGmxbjDhd5NigQ>? MWGyNVEzOTZ|MR?=
human HCT116 cells NE\HZ5RRem:uaX\ldoF1cW:wIHHzd4F6 NWXQSYZJPzJiaB?= M{LrfGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZYJ{d3KkYX7j[UBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUWxMlgzKM7:TT6= Mm\INlU3QTN5OEe=
human HCT116 cells NWmxUmE{S3m2b4TvfIlkyqCjc4PhfS=> NXjtOHdRPzJiaB?= MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME21Ok4xOSEQvF2u NH7XRokzOTl2NUK1NC=>
human PC3 cells NEm3[olEgXSxdH;4bYPDqGG|c3H5 NIqzUoY4OiCq M4L3dWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPU[xMlM2KM7:TT6= M1TWV|IzPDhyOEWx
human HuH7 cells MlLEVJJwdGmoZYLheIlwdiCjc4PhfS=> MXW3NkBp MUnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjYoPvdoJidmOnIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PjdwMUig{txONg>? MWqyOVY6Ozd6Nx?=
human A549 cells MoLkVJJwdGmoZYLheIlwdiCjc4PhfS=> NHX0N2Q4OiCq M2fmW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHid49z[mGwY3WgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD16Mj6zNkDPxE1w M1nBUVI2Pjl|N{i3
human A549 cells MnK1R5l1d3SxeHnjxsBie3OjeR?= M3jpR|czKGh? NYHO[Yp3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6 M1e4dlIzPDhyOEWx
human PC3 cells MkL6VJJwdGmoZYLheIlwdiCjc4PhfS=> M3TITlI2KM7:TR?= M4DjbFEzOCCq MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjdDCyOUB2VSCjZoTldkAyOjBiaILzJIJ6KE2WVDDhd5NigSC{ZXzheIl3\SC2bzDEUXNQ M2LkcVE3PjhyMUW5
human H460 cells M4q4Z2Z2dmO2aX;uJIF{e2G7 MXKzNEDPxE1? NF\oUGVKdmirYnn0bY9vKG:oIGDJN2shcW5iaIXtZY4hUDR4MDDj[YxteyCjc4Pld5Nm\CCjczDBb5QheGixc4Doc5J6dGG2aX;uJIF1KFOncjC0O|MhfXBidH:gN|AhfU1? NVTVbXZbOTh3MEG2NFE>
human IGROV1 cells Ml;NSpVv[3Srb36gZZN{[Xl? MY[yOEBp NFLobmpE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBKT1KRVkGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFexJJBp[XOnIHH0JFEh\m:uZDDJR|UxKGGodHXyJFI1KGi{czDifUBndG:5IHP5eI9u\XS{eR?= NVfOR2g3OjF{MU[xOVE>
human IGROV1 cells MoD4SpVv[3Srb36gZZN{[Xl? Ml\QNlQhcA>? MkThR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gTWdTV1ZzIHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMTDwbIF{\SCjdDC1JIZwdGRiSVO1NEBi\nSncjCyOEBpenNiYomg[oxwfyCleYTvcYV1enl? MWKyNVIyPjF3MR?=
human H4 cells M{TQfGZ2dmO2aX;uJIF{e2G7 M{S3OlIhcA>? M2e0NWRm[3KnYYPlJIlvKE[\VlWtVmZRMyC4ZYPpZ4xmKGmwdHXud4l1gSCyZYKgZ4VtdCCrbjDoeY1idiCKNDDj[YxteyCjZoTldkAzKGi{czDy[YxifGm4ZTD0c{Bkd262cn;s MnjRNVgxOjR3OES=
human MGC803 cells NFuxdWZHfW6ldHnvckBie3OjeR?= NW\jUJA3OjVizszN NVnlfHlHPDhiaB?= NETid3RE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBOT0N6MEOgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFeyM20heGijc3WgZZQhOjVidV2gZYZ1\XJiNEigbJJ{KHW|aX7nJJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnegZYZ1\XJiNEigbJJ{KGK7IH\sc5ch[3m2b33leIVzKHKnbHH0bZZmKHSxIHPvcpRzd2x? M1POdFI1OTF7OE[5

... Click to View More Cell Line Experimental Data

In vivo試験 LY294002 also results in suppression of tumor growth and induction of apoptosis, especially in the LoVo tumors, and therefore shows remarkable effectiveness in the mouse peritonitis carcinomatosa model. [2] LY294002 significantly inhibits growth and ascites formation of ovarian carcinoma. [3]

プロトコル(参考用のみ)

キナーゼアッセイ:[4]
+ 展開

kinase assays:

PI3K inhibition by LY294002 is determined in a radiometric assay using purified, recombinant enzymes with 1 μM ATP. The kinase reaction is carried out for 1 hour at room temperature (24oC) and is terminated by addition of PBS. IC50 values are subsequently determined using a sigmoidal dose–response curve fit (variable slope). CK2 and GSK3β (glycogen synthase kinase 3β) inhibition is established by kinase selectivity screening. LY294002 is tested against the Upstate panel of kinases in 10 μM ATP.
細胞アッセイ: [2]
+ 展開
  • 細胞株: Colon cancer cell lines DLD-1, LoVo, HCT15, and Colo205
  • 濃度: 0–50 μM
  • 反応時間: 0–48 hours
  • 実験の流れ: 1.0×105 cells (100 μL volume/well) are inoculated into 96-well microtiter plates. LY294002 is added to triplicate wells and cultured at 37oC for 0–48 hours. After treatment, 10 μL of Premix WST-1 are added to each microculture well, and the plates are incubated for 60 minutes at 37oC, after which absorbance at 450 nm is measured with a microplate reader.
    (参考用のみ)
動物実験:[3]
+ 展開
  • 動物モデル: Two groups of athymic nude mice (5–7 weeks) are inoculated i.p. with OVCAR-3 cells
  • 製剤: Dissolved in DMSO plus 0.25 ml of PBS
  • 投薬量: 0–100 mg/kg
  • 投与方法: Administered via i.p.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 36 mg/mL (117.13 mM)
Ethanol 21 mg/mL (68.32 mM)
Water <1 mg/mL
体内 4% DMSO+30% PEG 300+5% Tween 80+ddH2O 5mg/mL

* <1 mg/mlは製品が微弱に溶解する或いは溶解しないことを示します。
* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 307.34
化学式

C19H17NO3

CAS No. 154447-36-6
保管
in solvent
別名 SF 1101, NSC 697286

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    I want to buy a inhibitor that can inactivates Akt/PI3K. I notice the protocol of LY294002 (catalog no. s1105) says it will inactivates Akt/PKB. Is LY294002 available for Akt/PI3K inhibition?

  • 回答:

    LY294002 is the first synthetic molecule known to inhibit PI3Kα/δ/β with IC50 of 0.5 μM/0.57 μM/0.97 μM, respectively. It can inhibit PI3K/AKT. PKB is the alternative name of AKT.

  • 問題2:

    What is the suggested formulation of this compound for mouse injection(i.p.)?

  • 回答:

    It can be dissolved in 4% DMSO/30% PEG 300/5% Tween 80/ddH2O at 5 mg/ml clearly, and the concentration of DMSO is safe for in vivo experiments.

PI3K信号経路図

PI3K Inhibitors with Unique Features

相関PI3K製品

Tags: LY294002を買う | LY294002 ic50 | LY294002供給者 | LY294002を購入する | LY294002費用 | LY294002生産者 | オーダーLY294002 | LY294002化学構造 | LY294002分子量 | LY294002代理店
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID