Doxorubicin (Adriamycin) 化学構造
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製品説明

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  • 研究分野
  • Combination Therapy
    併用療法

製品の説明

生物活性

製品説明 Doxorubicin (Adriamycin) は、DNAトポイソメラーゼIIを妨げて、DNAの損傷とアポトーシスを誘発する抗生エージェントです。
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IC50
In vitro試験 Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
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RPMI-6666 M1rGXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfkeXJKSzVyPU[1Mlk6KG6P NVr2V21mW0GQR1XS
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CAL-33 M13uOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HoVWlEPTB;NkeuPFchdk1? MVvTRW5ITVJ?
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Daudi NIWxeoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTZ6Lkm2JI5O MnrBV2FPT0WU
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TUR NELUcXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1e5RmlEPTB;N{[uN|Ihdk1? MnLnV2FPT0WU
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NCI-H1650 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHlVGRKSzVyPUe4MlYzKG6P NEjCb4NUSU6JRWK=
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SW1783 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\lOpNKSzVyPUm3Mlk6KG6P NYr0[4dFW0GQR1XS
CAL-39 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13TfmlEPTB;OUiuNlQhdk1? NIC3XI1USU6JRWK=
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MES-SA MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTl7LkWzJI5O M3jlb3NCVkeHUh?=
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AU565 NH;WZ3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTFyND61NUBvVQ>? NWm2cGduW0GQR1XS
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MMAC-SF NXnLZZBvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MorNTWM2OD1zME[uOFghdk1? NF7J[4tUSU6JRWK=
ChaGo-K-1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTo[VBWUUN3ME2xNFcvQTVibl2= NVzqeJU5W0GQR1XS
NCI-H596 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TkUWlEPTB;MUC4MlAyKG6P MoD0V2FPT0WU
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NCI-H2170 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\sfWlEPTB;MUC5MlkzKG6P NVLsNJI4W0GQR1XS
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SJSA-1 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTFzMT6wOUBvVQ>? MUnTRW5ITVJ?
SW626 NFvSSndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rofmlEPTB;MUGxMlcyKG6P M2rES3NCVkeHUh?=
EHEB M3P1Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3qTWM2OD1zMUKuOVkhdk1? MkDJV2FPT0WU
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MZ7-mel MljCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HTb2lEPTB;MUGyMlg1KG6P NHXScY9USU6JRWK=
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TK10 Mn3CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTFzND62JI5O NIPpN|dUSU6JRWK=
SW13 NXPaV49iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlX1TWM2OD1zMUWuNlYhdk1? M2CxWHNCVkeHUh?=
HCC1937 MnSyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLnTWM2OD1zMUWuPFQhdk1? MW\TRW5ITVJ?
SK-NEP-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTFzNj6yOkBvVQ>? MkPwV2FPT0WU
OVCAR-5 NVzLZYZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGjlSppKSzVyPUGxOk45OSCwTR?= MVvTRW5ITVJ?
SW837 Mom5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTMRYZKSzVyPUGxO{46PSCwTR?= NVi5XFk5W0GQR1XS
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SW872 NI\iOoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlOxTWM2OD1zMkCuPFEhdk1? M4DTW3NCVkeHUh?=
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LB647-SCLC MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDHTWM2OD1zMkOuN|Uhdk1? NGrXNHVUSU6JRWK=
NCI-H1703 NVXIR5NPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTF{Nj60NkBvVQ>? NGjaWZlUSU6JRWK=
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SK-CO-1 M4e3cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PpdGlEPTB;MUK5MlIyKG6P NG\XWHJUSU6JRWK=
RPMI-7951 NFLHcXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjOTWM2OD1zM{CuNVQhdk1? MVvTRW5ITVJ?
NCI-H1648 NWjifYpkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3j3XmlEPTB;MUOxMlk1KG6P MmP1V2FPT0WU
NCI-H64 NH\kNpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTF|Mj62OUBvVQ>? MVHTRW5ITVJ?
HT-3 M1PWNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXrO2IxUUN3ME2xN|MvODRibl2= MUPTRW5ITVJ?
NCI-H1770 NXzJZ2lHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XQXWlEPTB;MUOzMlIyKG6P NHzJ[VZUSU6JRWK=
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NCI-H747 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTF|NT6wNkBvVQ>? M33VcHNCVkeHUh?=
ML-2 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nhVWlEPTB;MUO1MlE3KG6P M4TSXXNCVkeHUh?=
CA46 NG[1T21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LnUGlEPTB;MUO2Mlg5KG6P MU\TRW5ITVJ?
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JiyoyeP-2003 M2jremdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEfqcINKSzVyPUGzO{45KG6P MlPnV2FPT0WU
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NCI-H748 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\LV2lEPTB;MUS3MlMyKG6P NUL4d2k5W0GQR1XS
SK-MEL-3 NH31[INIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPaXHBKSzVyPUG0O{43OSCwTR?= M2XmUnNCVkeHUh?=
SNB75 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTF2OD62OUBvVQ>? MnjyV2FPT0WU
BB65-RCC NWTIO|JrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\KZ4JCUUN3ME2xOFkvOzFibl2= NEnnd2RUSU6JRWK=
NCI-H1355 NGXRWFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE[5ZmtKSzVyPUG1Nk4zPCCwTR?= MXHTRW5ITVJ?
SCC-25 M{G2NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrKTWM2OD1zNUOuO|Mhdk1? MV;TRW5ITVJ?
OAW-42 NF;ZUY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjFTWM2OD1zNUOuPFQhdk1? Mn[wV2FPT0WU
MONO-MAC-6 NHvFN4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTF3ND6zOkBvVQ>? MmPUV2FPT0WU
WSU-NHL MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTF3ND61PEBvVQ>? Mlu3V2FPT0WU
SNU-423 Moj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzCTWM2OD1zNUWuN|Qhdk1? Mo\oV2FPT0WU
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OVCAR-4 MnTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWe4dFZXUUN3ME2xOVYvPzhibl2= MkPqV2FPT0WU
NCI-H2347 NGriSHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTF3OD6zOEBvVQ>? M{TqcnNCVkeHUh?=
CAMA-1 NFu4UoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn2zTWM2OD1zNUmuN|Mhdk1? M{DKZ3NCVkeHUh?=
GDM-1 NHvOOXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jsT2lEPTB;MUW5Mlchdk1? M1S4ZnNCVkeHUh?=
KNS-81-FD NF7iO4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFX4VoNKSzVyPUG2NE4{OiCwTR?= MlfnV2FPT0WU
no-10 Ml\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTF4MD6zPEBvVQ>? NEPmOFBUSU6JRWK=
NCI-H2052 NUHJ[pBDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnRV3pKSzVyPUG2NE41PyCwTR?= MonxV2FPT0WU
DB NXTh[llmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;SS|VKSzVyPUG2NU44OSCwTR?= Mkm1V2FPT0WU
A4-Fuk NF;NS|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7XTWM2OD1zNkKuOFghdk1? NVXifm4{W0GQR1XS
HCE-T M{[yfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjOTWM2OD1zNkSuPFchdk1? MWrTRW5ITVJ?
HuP-T3 M1;VPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjzfYxqUUN3ME2xOlUvPTVibl2= NXzkd2hYW0GQR1XS
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A704 MnHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LyWGlEPTB;Mj6yPVE{PSEQvF2= MUHTRW5ITVJ?
EVSA-T NYTzfW1wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXod4RKSzVyPUKuN|AxQDlizszN NH:3VXlUSU6JRWK=
HCT-15 NV\3[nc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXBT5RKSzVyPUKuN|U1PjZizszN NXTsOFFCW0GQR1XS
HuO-3N1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHfLd4dKSzVyPUKuN|Y2QDNizszN NUHpfZUxW0GQR1XS
GMS-10 NIjSeWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PwXmlEPTB;Mj6zPVc{QCEQvF2= MnLXV2FPT0WU
TGBC24TKB NF;a[mdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrRTJhKSzVyPUKuOFE1OjlizszN MXXTRW5ITVJ?
C2BBe1 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DScGlEPTB;Mj61NVMzQCEQvF2= M4LwRnNCVkeHUh?=
SK-N-DZ M3:zOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTJwNU[1PVIh|ryP NYfDR4luW0GQR1XS
SK-N-AS MkLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;CTWM2OD1{LkezNFE2KM7:TR?= NUDqNmpbW0GQR1XS
T98G Mmm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTJwOEC0NFEh|ryP MVfTRW5ITVJ?
DMS-79 M4XtT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWm4XpJjUUN3ME2yMlkxQTh6IN88US=> MlK4V2FPT0WU
SNU-475 M4jQN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vIdGlEPTB;Mj65NlU4OiEQvF2= MYLTRW5ITVJ?
COLO-792 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHvTWM2OD1|LkCyPFch|ryP Mlf0V2FPT0WU
YAPC M2LxSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTNwM{K4N|Ih|ryP MVPTRW5ITVJ?
UACC-812 NF7qVVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTqTWM2OD1|LkOzO|M5KM7:TR?= NIG4bJhUSU6JRWK=
Mewo MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3QZXpLUUN3ME2zMlQ3ODh4IN88US=> M4LjZXNCVkeHUh?=
COLO-824 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTNwNUezO|gh|ryP M3z5RXNCVkeHUh?=
SCC-9 M2fBOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTNwOUGzNFkh|ryP NHjEe2pUSU6JRWK=
CP50-MEL-B MkniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTNwOUi3NFMh|ryP M{nxXnNCVkeHUh?=
NCI-H345 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHlTWM2OD12LkCxNVc1KM7:TR?= M4HDVHNCVkeHUh?=
COR-L88 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTRwM{m1PEDPxE1? MnPoV2FPT0WU
RCM-1 MnvhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTRwNEmyO|Ih|ryP MnvDV2FPT0WU
JVM-3 NITGbItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XiOWlEPTB;ND62NFYyQCEQvF2= M{fq[nNCVkeHUh?=
ECC4 NH3SbGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTRwN{[1OFEh|ryP NEHlclNUSU6JRWK=
MDA-MB-453 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYTaeGlWUUN3ME20Mlg2OTN2IN88US=> M1XNenNCVkeHUh?=
SK-N-FI Mly2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTVwMkK4O|ch|ryP NWHEV2tIW0GQR1XS
D-502MG M1zBOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDxOodKSzVyPUWuNlQzPjJizszN MmqwV2FPT0WU
NCI-H2227 NEDhfWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfFTWM2OD13LkO2NlMyKM7:TR?= MYDTRW5ITVJ?
DMS-53 NIXZXXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13XNWlEPTB;Nj6xO|ExPiEQvF2= MXzTRW5ITVJ?
LB771-HNC NHjld3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXDTWM2OD14LkS2PFA3KM7:TR?= MXHTRW5ITVJ?
BB49-HNC M{fkUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPlWZZMUUN3ME23MlQyPzlzIN88US=> NGTlbG5USU6JRWK=
MDA-MB-415 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HN[WlEPTB;Nz61O|MxOyEQvF2= M37WeHNCVkeHUh?=
TALL-1 NVS1bnFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTdwOU[4PEDPxE1? MkH2V2FPT0WU
HCC1395 MkKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPTTWM2OD16LkG1NVIzKM7:TR?= MlXNV2FPT0WU
EFE-184 MlO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnpTWM2OD16LkO3NlE3KM7:TR?= M{HuOXNCVkeHUh?=
EC-GI-10 NHPldG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTQTWM2OD16Lk[4Olg3KM7:TR?= M3zHW3NCVkeHUh?=
TT M2q4Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;4[WlqUUN3ME25MlA3QTZ4IN88US=> Mof1V2FPT0WU
U-2-OS NFP2PFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoThTWM2OD17LkixN|k{KM7:TR?= MU\TRW5ITVJ?
SHP-77 NVTrfFZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUL2UnpiUUN3ME25MlkxOjlizszN NVXqdXZZW0GQR1XS
HCC1143 NH7sdWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2izd2lEPTB;MUCuOFc6PyEQvF2= MUnTRW5ITVJ?
A498 NGTLXXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlO1TWM2OD1zOD6yPVc3KM7:TR?= MoK4V2FPT0WU
LS-513 M1m2Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoqyTWM2OD1zOD62NFk{KM7:TR?= MXnTRW5ITVJ?
K052 NFPZXI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{L0OWlEPTB;MkGuOFQyQCEQvF2= NG\PZmVUSU6JRWK=
SiHa MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmS0TWM2OD1{Mz6zN|cyKM7:TR?= Mn\sV2FPT0WU
RCC10RGB M2C2Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrUTWM2OD1|MD6wOlEyKM7:TR?= M1PF[nNCVkeHUh?=
NCI-SNU-16 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTNzLkG4N|Yh|ryP NYTESY9XW0GQR1XS
SNU-C1 MnnsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\JN5FKSzVyPUOyMlQyPzVizszN NXSxdoJ6W0GQR1XS
C32 M3PLTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\qTWM2OD1|Mj64O|Ih|ryP MVTTRW5ITVJ?
SW684 MlzDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\6d4dHUUN3ME2zOE4yPTZzIN88US=> NXvFTHR7W0GQR1XS
SK-MEL-28 NGHwPHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfZfIFRUUN3ME2zOU42Ozd5IN88US=> M2fOfnNCVkeHUh?=
SK-MEL-1 M{fidGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXOdmpOUUN3ME2zOk44PTR5IN88US=> NVfaNFJ7W0GQR1XS
NCI-H720 NE\icXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTRzLkm3NlYh|ryP MnjNV2FPT0WU
P31-FUJ Ml[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPocIZKSzVyPUS0MlMzQDRizszN NGPjcndUSU6JRWK=
NCI-H524 NF;ScllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrKV3dKSzVyPUS4MlgzPDhizszN M2XBSHNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo試験 In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]
臨床試験 A Phase III study of Doxorubicin alone vs. Doxorubicin in combination with TH-302 in subjects with locally advanced unresectable or metastatic soft tissue sarcoma is currently ongoing.
特集

プロトコル (参考用のみ)

動物実験: [1]

動物モデル Female athymic nude mice injected s.c. with MB231 cells
製剤 Dissolved in DMSO, and diluted in saline
投薬量 3 mg/kg/day
投与方法 Delivered intratumorly

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDogMonkeyBaboon
Weight (kg)0.020.151.80.40.0810312
Body Surface Area (m2)0.0070.0250.150.050.020.50.240.6
Km factor361285201220
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

参考

化学情報

Download Doxorubicin (Adriamycin) SDF
分子量 579.98
化学式

C27H29NO11.HCl

CAS No. 25316-40-9
保管 2年-20℃
6月-80℃in solvent
別名 NSC 123127
溶解度 (25°C) * In vitro DMSO 100 mg/mL warming (172.41 mM)
20 mg/mL (34.48 mM)
エタノール <1 mg/mL (<1 mM)
In vivo
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
化学名 (8S,10S)-10-((2R,4S,5S,6S)-4-amino-5-hydroxy-6-methyl-tetrahydro-2H-pyran-2-yloxy)-6,8,11-trihydroxy-8-(2-hydroxyacetyl)-1-methoxy-7,8,9,10-tetrahydrotetracene-5,12-dione hydrochloride

カスタマーフィードバック (6)


Click to enlarge
Rating
Source , , Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) purchased from Selleck
Method Apoptotic Response Assay
Cell Lines MDA-MB-231 cells
Concentrations 10 mg/kg
Incubation Time 24 h
Results The apoptotic response inMDA-MB-231 (human triple-negative breast) tumors, as tested by the device, was significantly elevated to 17.5% from 7.4%by the addition of lapatinib to doxorubicin in reservoirs.

Click to enlarge
Rating
Source Cancer Res, 2014, 74, 298-308. Doxorubicin (Adriamycin) purchased from Selleck
Method MTT assay
Cell Lines H1299, A549, Hep3B, HT1080, HCT116, MCF7
Concentrations
Incubation Time 48 h
Results Hypoxic (1% O2 ) conditions for 48 hours. The IC50 value for cisplatin or doxorubicin significantly increased under hypoxia in H1299, A549, Hep3B, and HT1080, but did not in HCT116 and MCF7

Click to enlarge
Rating
Source J Biomed Mater Res Part A, 2012, 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) purchased from Selleck
Method Release experiment
Cell Lines N.A.
Concentrations 0.25mg/mL
Incubation Time 2-10 h
Results Doxorubicin was loaded by equilibrium partitioning in the following manner: a stock solution of doxorubicin was prepared in 2 wt % DMSO in 1 PBS (pH 7.4) at a concentration of 0.25 mg/mL. A 5 mg/mL concentration of crushed particles (75–150 um) of P(MAA-g-EG) or P(MAA-g-EG) containing PMMA nanoparticles to doxorubicin stock solution was allowed to stir slowly for 2 h.

Click to enlarge
Rating
Source PLoS One, 2014, 9, e94309. Doxorubicin (Adriamycin) purchased from Selleck
Method Flow Cytometry Analysis
Cell Lines K-562, DL cells
Concentrations 5.85 uM
Incubation Time 24 h
Results It was extended our observation of DOX-uptake at microscopic level for visualizing the presence of significantly higher DOX concentration intracellularly in K-562 and DL. As observed in I and J, the intracellular level of DOX is significantly higher in cells treated with DOX-PCL63-b-PNVP90 compared to free DOX suggesting high drug delivery efficiency of the compound.

Click to enlarge
Rating
Source PLoS One, 2013, 8(1), e54595. Doxorubicin (Adriamycin) purchased from Selleck
Method MTT assay
Cell Lines Huh7, THLE2, Hep5B cells
Concentrations 0.016-52 uM
Incubation Time 74 h
Results Cytotoxicity by doxorubicin was measured by MTT assay atvarying concentrations on the HCC lines Huh7, THLE2, Hep3B.

Click to enlarge
Rating
Source 2014, Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) purchased from Selleck
Method Transwell membranes assembled
Cell Lines COR-L23, RH460 cells
Concentrations 50 nM, 1 uM
Incubation Time 24 h
Results Evaluation of DOX effect on cancer cell invasion in combination with inhibitors of CXCR4 receptor (WZ811) and focal adhesion kinase (FAK; PF-573228). Two DOX resistant non-small cell lung carcinoma (NSCLC) cell lines were employed: COR-L23 that shows intrinsic resistance to DOX and RH460 with classical acquired multidrug resistant phenotype. The results show that DOX, classical cytotoxic drug, potentates known anti-migratory effects of CXCR4 receptor inhibitor (WZ811) and FAK inhibitor (PF-573228) in both types of resistant NSCLC cell lines. WZ811 had more pronounced and significant effect with DOX in RH460 cells while PF-573228 was more effective with DOX oin COR-L23 cell line.

文献中の引用 (28)

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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